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T Hermanowska-Szpakowicz

Publications and source records attributed to T Hermanowska-Szpakowicz.

At least 37 records · Page 2Linked to original sources
PubMed2000

Analysis of some peripheral blood lymphocyte subsets in relation to Borrelia burgdorferi antibodies in patients with Lyme disease.

The aim of our study was to evaluate the changes in T (CD3), B(CD19) lymphocytes, CD4 and CD8 subsets, activated CD3+ HLA-DR+ lymphocytes, lymphocytes with receptor for IL-2 (CD3+CD25), NK cells as well as the rate of CD4/CD8 in 30 patients with recognized Lyme disease, before and after antibiotic therapy. Patients were divided into the following groups: Group I n = 9--without detected specific antibodies against B. burgdorferi and with clinical recognition of EM(erythema migrans). Group II n = 10--with increased IgM production, with a clinical form of Lyme arthritis and neuroborreliosis. Group III n = 11--with increased IgG anti B. burgdorferi production, with clinical recognition of Lyme arthritis. The results were compared with the results obtained in the control group consisting of 90 healthy people. The measurements were performed in the flow cytometer COULTER EPI XL with Becton Dickinson antibodies. The antibodies against B. burgdorferi were detected by means of ELISA method using Dako, Biomedica and Biocom kits. The statistic analysis was performed with AnStat Program. The changes in lymphocyte subsets were characterised by the decrease in the percentage of CD4, CD8, NK and CD3+HLA-DR+ lymphocytes in peripheral blood before treatment with later tendency to increase. The results show that the lag phase of antibodies production coincides with high activity of lymphocytes (group I). The significant level of antibodies in IgM class induced by the collaboration of T and B cells was found in the group II. In the group III, in which antibodies in IgG class predominated, the changes in lymphocyte subsets were less intensive. The results of our investigations indicate that the immune response in Lyme disease develops mainly with the participation of both cellular and humoral response which is involved in both the defense against and the pathogenesis of the disease.

Adolescent↗
PubMed1999

[Selected lymphocyte subsets in Lime borreliosis: a preliminary study].

Observation of percentages changes of lymphocytes (CD19), T(CD3), subsets CD4, CD8, NK cells, activated lymphocytes with fenotype CD3+HLA-DR, CD4/CD8 rate in Lyme borreliosis was carried out before (I examination) and after antibioticotherapy (II examination). 30 patients in aged 17-60 (x = 41) with recognition of erythema migrans(EM), Lyme arthritis(LA) and neuroborreliosis(NB) were examined. Epidemiological, clinical diagnosis was confirm by the presence of antibodies in ELISA test. Lymphocytes and their subsets, NK cells were signed and measured by flow cytometric immunophenotyping in Coulter EPIX XL with Becton-Dickinson antibodies twice: before and after treatment. Ampicillin, ceftriaxon or cefotaxim was applied during 4 weeks. AnStat program was used in statistic analysis. Value CD3 (x = 72.54) in I test was higher than control (x = 69.3), but CD19 (x = 13.2) was lower than control (x = 12.9). In II examination we stated CD19 (x = 9.48) progressive significant decreased in comparison to I examination. Percentage CD4 in II examination (x = 42) was lower than control (x = 45.8). Subset of CD8 had lower value in I examination (x = 28.03), as in II (x = 30.72) in comparison with control (x = 34.2). We showed lower CD4/CD8 (x = 1.40) rate after treatment than control (x = 2.67). We showed lower percentage NK cells after treatment (x = 13.5) than control. We also found lower percentage activated subsets T cells with phenotype CD3+HLA-DR before (x = 4.78) and after treatment (x = 4.03) compared with control (x = 7.27). No statistical changes in subsets with receptor for IL-2 before and after treatment was shown. In the course of active infection B.burgdorferi essential changes in lymphocytes subsets are observed. Decreased percentages of CD4, CD8, NK and CD3+HLA-DR+ in whole blood indicate their important role in immunopathogenesis of Lyme borreliosis. Lack of normalization of investigated parameters after treatment can be caused by inertion of elements immunologic system, as well as too short antibioticotherapies.

Adolescent↗
PubMed1999

[Herpes simplex encephalitis].

Herpes simplex virus infection of the central nervous system is still a significant cause of morbidity and often mortality. Changes of central nervous system are results of primary infection or activation of latent HSV-1, HSV-2. Neurological deficits often follow encephalitis herpetica. The application of PCR is prompt and specific diagnosis of herpes simplex virus infections of the brain. Advances in treatment herpes simplex encephalitis with acyclovir have improved outcome.

Acyclovir↗
PubMed1999

[Lyme disease vaccine: hopes and concerns].

The vaccine against Lyme disease for last decade became an important issue for many researchers. Recently two groups of scientists published data of their trials carried out on humans. The vaccine which was given to volunteers consisted of recombinant outer surface protein (Osp)A. These people were observed for two years when approximately 79% of them developed resistance to Borrelia burgdorferi infection. These data were evaluated by FDA experts who recommended vaccine for approval. Main concerns of experts are: short duration of observation of vaccinated people, no possibility to vaccinate children, problematic vaccination people with undiagnosed Lyme disease. However the moment when effective and safe vaccine against Lyme disease will be available seems to approach.

Adolescent↗
PubMed1999

[Epidemiology of Lyme borreliosis].

In this paper basic issues concerning epidemiology of Lyme disease were presented. The role of ticks and small mammals was emphasised. An increasing prevalence of Lyme disease especially among people working in forests was pointed out.

Humans↗
PubMed1999

[Prevention of Lyme disease].

Over the last decade many researchers have turned much attention to the vaccine against Lyme disease as the only effective preventive method. Recently two groups of researchers have published data on their trials carried out on humans. The vaccine given to volunteers consisted of recombinant outer surface protein (Osp)A. A two-year observation of the persons studies revealed that 79% of them had developed resistance to Borrelia burgdorferi infection. These data were evaluated by the FDA experts who recommended the vaccine for general use with the following reservations that have to be elucidated: too short observation of vaccinated persons, no possibility to vaccinate children and the problem of vaccinating people with undiagnosed Lyme disease.

Adolescent↗
PubMed1999

[Levels of proinflammatory cytokines: IL-1, IL-6, IL-8, TNF-alpha and receptor IL-6R in Lyme borreliosis].

We estimated serum concentrations of cytokines IL-1, IL-6, IL-8, TNF-alfa and IL-6R of patients with diagnosed Lyme disease treated with beta-lactam antibiotics. Detection of proinflammatory cytokines was performed in ELISA tests. The examination was performed before and after treatment. Comparison with control group stated statistically significant higher concentration of IL-1 and IL-6 before and after treatment. There were no differences in concentration of TNF-alfa, IL-8 and IL-6R. Comparing concentrations of cytokines before and after treatment there was no differences either. Lack of changes in concentration of proinflammatory cytokines during beta-lactam therapy could be explained by too short period of therapy or immunologic background of inflammatory process in Lyme disease which was only initiated by spirochete Borrelia burgdorferi.

Adult↗
PubMed1999

[The time of maintainance of positive serologic results among patients operated on echinococcal liver cysts].

The purpose of this work was to analyse results of serologie examinations and complaints reported by patients who were operated on echinococcal liver cysts. The analysis was conducted on patients who were qualified to surgical removal of echionococcal liver cysts. Persons who were analysed answered the questionaires and 11 of them send sera for serologie examination. The questionaires consisted of questions about time since operation, complaints before and after operation abdomen pains, distensions, vomits, exanthema, weakness, headaches). Results showed that patient were from 1 to 6 years after operation. Among 11 who send sera 18% results were negative (2 and 6 years after operation) and 82% was positive. Analysis of 15 questionnaires showed that majority of patients still had the complaints that were present before operation.

Adult↗
PubMed1998

[Concentrations of tumor necrosis factor alpha and interleukin-1 beta in cerebrospinal fluid in the course of tick-borne encephalitis].

CSF concentrations of TNF-alpha and Il-1 beta were detected in patients with TBE. The cytokines were detected by immunometric assay by MEDGENIX kit. CSF Concentrations of TNF-alpha and IL-1 beta in patients with TBE were significantly higher than in control group before as well as after treatment and normalization of CSF parameters. These concentrations were lower comparing to one obtained in group of bacterial meningitis. There was no correlation between concentration of cytokines and other CSF parameters (cytosis, protein, glucose concentration). Concentrations of analysed cytokines did not change significantly before and after treatment. Detection of CSF concentrations of TNF-alpha and Il-1 beta in patients with tick-borne encephalitis can be used to evaluate efficacy of treatment and retreat of infection.

Adolescent↗
PubMed1998

[Detection of antibodies to Borrelia burgdorferi among forestry workers in North-Eastern Poland].

The purpose of the study was to assess the incidence of Borrelia burgdorferi antibodies and clinical forms of Lyme borreliosis among forestry workers in north-eastern Poland. The group studied consisted of 1,466 persons (297 women and 1,169 men), aged 20-70 years. In 439 (23.81%) persons the presence of B. burgdorferi antibodies was detected in serum, in 271 (18.49%) persons active Lyme borreliosis was diagnosed and 78 (5.32%) persons were carriers of B. burgdorferi antibodies. Arthritis (43.84%) and neuroborreliosis (32.95%) were found to be the most common forms of borreliosis.

Adult↗
PubMed1998

[Otoneurologic state estimation 2 years after tick-borne encephalitis].

In this paper an attempt is described to estimate the incidence and frequency of neurologic and otologic symptoms among patients two years after TBE. We examined 43 persons of both sexes aged 17-58. The most frequent complaints were: headache--34.9%, equilibrium problems--37.2%, buzzing in the ears--27.9%, hearing problems--23.3%, memory problems--25.6%. Decrease of throat reflexes was stated in 3 (7%) and pseudobulbaris symptoms in 2 (4.6%), weakness of muscles in 4 (9.3%). In audiometric examination decrease of hearing was stated in 8 persons (18.5%). We registered nystagmus: spontaneous--4.6-7%, gaze--13.9-18.6%, positional-T, mainly Nylen I and III type-18.6-25.6%. Pathologic recording in the eye-tracking pattern test was shown in 7 (16.3%) persons. Asymmetry of optokinetic nystagmus was stated in 6 (13.9%) examined persons. Asymmetry in caloric test was proved in 25.5% of examined persons.

Adolescent↗
PubMed1997

[Albumin and immunoglobulin levels in cerebrospinal fluid and serum in tick borne meningoencephalitis].

The levels of albumin and immunoglobulins G, A, M were determined by using nephelometry technique in the cerebrospinal fluid and serum in 14 adults with tick borne meningoencephalitis at the beginning and after four weeks of disease. Intra blood-brain synthesis of IgG, IgA, IgM was evaluated by indexes of synthesis (Tibbling formula). The blood-brain integrity was estimated by CSF/serum albumin ratio. Increased albumin and immunoglobulins G, A, M levels were demonstrated in first examination and only elevated levels of albumin and immunoglobulin M lasted after four weeks. Albumin level in cerebrospinal fluid and albumin ratio were elevated in both examinations. The serum levels of albumin and immunoglobulins were similar in first and second examination. On the basis these results we conclude that in tested patients blood-brain integrity was disturbed still after four weeks of disease.

Adolescent↗
PubMed1996

[Detection of antibodies of Borrelia burgdorferi among inhabitants of north-eastern Poland].

The aim of this work was to estimate the detection of antibodies against Borrelia burgdorferi among inhabitants in North Eastern Poland 1765 persons from Białystok, Lomza and Suwałki voivodships were examined. Among them 1101 persons were from high-risk of exposition to ticks group (forest workers, people living close to forests). 418 (23.68%) persons from group of 1765 had antibodies against B.b. There was no difference of incidence of antibodies against B.b. in high-risk group and the others. The results show that North Eastern Poland is the endemic region of occurrence of B.b.

Adolescent↗
PubMed1996

[Tuberculous meningoencephalitis from personal material].

Clinical characteristics of 6 patients with tuberculous meningoencephalitis is presented. The increased morbidity of tuberculosis in recent years makes it necessary to consider this etiology in the diagnosis of CNS infections especially with severe atypic and long lasting course. In the case when tuberculous etiology is suspected the anti tuberculous treatment should be applied. Using new and modern diagnostic methods of CSF will allow to diagnose tuberculous meningoencephalitis earlier and will improve prognosis in this disease.

Adult↗
PubMed1996

[Analysis of selected parameters of damage to the liver during the course of tick-borne encephalitis].

In 1993, 80 patients (43 female and 37 male) with tick-borne encephalitis were hospitalized in Clinic of Neuroinfections and Parasitic Diseases of Medical School in Białystok. 6 (7.5%) patients were jaundiced, 16 (20%) had hepatomegaly. We noticed increased enzymatic activity of aspartate aminotransferase (x = 74.5 U/l) in 9 (11.25%) cases, alanine aminotransferase (x = 89.5 U/l) in 11 (13.75%) cases, increased level of bilirubin in blood (from 1.4 to 3.5 mg/dl) in 7 (8.75%) cases. Protein level in blood was average x = 5.95 g/dl, percentage of albumin was average x = 48.61%, globulin--x = 14.62%. Prothrombin index and ammonia blood level were in normal range. Observed pathological changes were transient and referred mainly to patients with severe clinical course of disease.

Adolescent↗
PubMed1995

[A comparison of serologic ELISA tests from BIOMEDICA Ges. M.B.H. and VIRO-Immun Labor Diagnostica GMBH in the diagnosis of Lyme disease (introduction)].

The purpose of this work was to evaluate detection of antibodies IgM and IgG against B. burgdorferi. We examined presence of IgM in 96 serum samples from patients suspected of Lyme disease. We looked for IgG in serum samples of 48 forest workers who submitted frequent tick bites in last two years. Among 96 patients whose sera were examined we had positive results in both tests in 5 cases and in 25 cases positive results were achieved only in test II. In 48 serum samples which we examined for presence of IgG in 2 cases positive results were in both tests, in 1 case positive in test II and negative in test I in 3 cases positive result in test I and negative in test II. According to these results we drew following initial conclusions: 1) obtained results testify better sensitivity and specificity of test II which consists of recombinant antigens of Borrelia burgdorferi, 2) no essential difference was shown in detection IgG among two used tests.

Enzyme-Linked Immunosorbent Assay↗
PubMed1994

[Epidemiological analysis of cases of TBE in 1993 in Bialystok district].

111 cases of TBE was 20 times increased morbidity in comparison to 1992 in Bialystok district in 1993. Among hospitalized dominated male patients (52.35%). First cases was reported in may and last in November with a peak in July. The age of patients was from 6 to 78 years old, 63.06%, in age group from 31 to 59 years old. We established that 78.87% patients were citizens of Bialystok, spending there recreation time in forest. Despite occurrence increased cases of TBE in 1993 we conclude that among people from risk group vaccination is indicated.

Adolescent↗