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Biomedical subjects

T Hildebrandt

Publications and source records attributed to T Hildebrandt.

11 recordsLinked to original sources
PubMed1999

Novel endotheliotropic herpesviruses fatal for Asian and African elephants.

A highly fatal hemorrhagic disease has been identified in 10 young Asian and African elephants at North American zoos. In the affected animals there was ultrastructural evidence for herpesvirus-like particles in endothelial cells of the heart, liver, and tongue. Consensus primer polymerase chain reaction combined with sequencing yielded molecular evidence that confirmed the presence of two novel but related herpesviruses associated with the disease, one in Asian elephants and another in African elephants. Otherwise healthy African elephants with external herpetic lesions yielded herpesvirus sequences identical to that found in Asian elephants with endothelial disease. This finding suggests that the Asian elephant deaths were caused by cross-species infection with a herpesvirus that is naturally latent in, but normally not lethal to, African elephants. A reciprocal relationship may exist for the African elephant disease.

Africa↗
PubMed1998

Analysis of parotid and mixed saliva in Roe deer (Capreolus capreolus L.).

In ruminants, different functions have been ascribed to the different salivary glands according to the feeding type. In this context, possible adaptations of salivary functions were investigated regarding the secretion of various proteins by different types of salivary glands. To yield uncontaminated parotid saliva in large quantities, a non-surgical method has been developed. Parotid gland secretions were collected via endoscopic placement of guide wires into each parotid duct, which were subsequently used for placement of collection catheters. Salivary flow was stimulated by intra-glandular administration of the parasympathomimetic compound pilocarpine-hydrochloride into the parotid gland. Mixed saliva (excluding parotid saliva) was collected into sterile tubes by normal outflow during the sampling of parotid saliva. The total flow volume, flow rate and the content of proteins as well as of several ions (Na+, K+, Ca2+, inorganic phosphate) of both types of saliva were measured in sheep, fallow deer and roe deer. Roe deer secreted the highest amount of total salivary proteins relative to body mass [mg/kg body mass] and the highest relative volume [ml/10 min/kg body mass], both in parotid and mixed saliva, of all ruminant species examined. Additionally, the protein profile and the tannin-binding properties of parotid and mixed saliva in roe deer were investigated. Parotid saliva bound almost twice as much tannin as mixed saliva, underlining the importance of yielding uncontaminated parotid saliva for tannin-binding studies.

Adaptation, Physiological↗
PubMed1997

Transrectal ultrasonographic examination of the female urogenital tract in nonpregnant and pregnant captive bears (Ursidae).

The development of reliable methods for monitoring the reproductive cycle of bears is needed to optimise breeding management in captivity. The aim of the present study was to develop non-invasive procedures for obtaining basic data on the reproductive status of nonpregnant and pregnant bears. Eight female captive bears (five Ursus arctos arctos, two Tremarctos ornatus and one Melursus ursinus) were examined using transrectal adaptor ultrasonographic imaging. Plasma concentrations of progesterone and gestagens in faeces were determined simultaneously. The ultrasonographic appearance of the female urogenital tract was assessed during the nonbreeding season and verified in four adult bears post mortem. Pregnant bears were examined twice, as the reproductive physiology of bears is characterized by delayed implantation and pseudopregnancy. During embryonic diapause there was no difference between pseudopregnant and pregnant bears both by ultrasonographic imaging and endocrinological data. After implantation (early December) the pregnancies were visualised by ultrasonographic imaging, whereas plasma concentrations of progesterone and gestagens in faeces had not yet increased. In conclusion, both transrectal ultrasonographic imaging and gestagen monitoring in faeces are efficient methods for obtaining important data on reproduction in bears.

Animals↗
PubMed1996

[Mucociliary clearance of radically operated maxillary sinuses and effect of endoscopic ethmoid bone revision on the mucociliary system].

Twenty-six maxillary sinuses (of 20 patients) were studied following Caldwell-Luc procedures. Surgery had been performed between 1 to 27 years previously. Follow-up studies included nasal endoscopy, coronal computed tomography and camera-sequence scintigraphy. Findings demonstrated that normalization of disturbed mucosal function was possible after surgery. Indications for revision endoscopic sinus surgery are discussed, as are the limitations of surgery.

Ciliary Motility Disorders↗
PubMed1990

[Fractures of the head of the tibia. Evaluation of an East German multicenter study 1981-1985].

The successful therapy of fractures of caput tibiae has always been the "haut ecol" of every treatment of fractures. A study made by 52 surgical clinics of the GDR which examined 1,954 patients who were under medical therapy between 1981 to 1985 summarizes the results. The patients have been examined for a period of 4.5 years in average. The study comparing the results of conservative and surgical medical therapy shows that there is no difference between the results of both methods of medical therapy regardless whether all kinds of fractures or whether only the same kinds of fractures were compared. Even if we consider the impression-fractures we come to the same result although we were of the opinion up to now, that impression-fractures should be treated in a surgical way only. The results of the study make clear the necessity of a strong indication for a surgical treatment and also that an early functional conservative therapy has the same results.

Adult↗
PubMedDate not supplied

Identification of URIM, a novel gene up-regulated in metastasis.

URIM (Up-Regulated In Metastasis) was identified as a new gene by Differential Display Technique during investigation of the transcriptional pattern of a metastasizing (NMCL-1) and a non-metastasizing (530) human melanoma cell line. The protein is encoded by 206 amino acids with an isoelectric point of 10.4. In addition, URIM displays a putative nuclear localization signal and a putative leucine zipper suggesting that URIM may function as a nuclear protein. Expression of URIM in several normal tissues and tumor cell lines was studied by Northern blotting. Surprisingly, 17 fold increased steady-state mRNA levels for URIM were detected in three cell lines derived from bone marrow micrometastasis of mammary carcinoma and one mammary carcinoma cell line derived from ascites fluid compared to normal epithelial cells from mammary gland and two cell lines derived from primary mammary carcinoma. These findings indicate that expression of URIM might be deregulated in metastases of different types of tumors.

Amino Acid Sequence↗
PubMedDate not supplied

Transcriptional profiling of human mammary carcinoma cell lines reveals PKW, a new tumor-specific gene.

In order to identify genes associated with distinct stages of mammary carcinoma we have investigated the transcriptional profile of normal mammary gland epithelial cells, cell lines derived from primary tumors, from bone marrow micrometastases and from ascites fluid. mRNA's for ribosomal protein L41 and URIM (Up-Regulated In Metastasis) were consistently increased in all cells derived from metastatic lesions. mRNA for human secreted frizzled-related protein (hsFRP) was found to be dramatically down-regulated in all mammary tumor cells compared to non-transformed mammary gland epithelial cells. mRNA for Human Hypoxia Related Factor--2 (HRF-2) and a transcript including the human mitochondrial control region were significantly overexpressed in the cell lines derived from primary tumors and ascites fluid. A new gene, referred to as PKW, was only expressed in one of the primary tumor cell lines, the one derived from a medullary carcinoma. The small and the large transcript which are derived by differential splicing encode potential proteins comprising 95 aa and 130 aa, sharing 88 aa at the N-terminus. The IEP's suggest a nuclear localization of the proteins. Surprisingly mRNA for the new gene was detected only in the salivary gland, but not in other adult human tissues and a restricted panel of embryonic tissues. The same holds true for a panel of human tumor cell lines and cell lines derived from ductal mammary carcinoma. RT-PCR revealed expression of PKW in 4 out of 11 breast carcinomas.

Amino Acid Sequence↗