Biological activities of endotoxin from Bacteroides melaninogenicus.
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Biomedical subjects
Publications and source records attributed to T Hofstad.
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Lipopolysaccharide (LPS) extracted with phenol-water from four oral strains of Bacteroides melaninogenicus was found to be serologically active in precipitation and complement fixation tests and sensitized sheep erythrocytes to agglutination. Except for the capacity to inhibit indirect hemagglutination, the serological activity was destroyed by oxidation with periodate. The isolated LPS was antigenic in rabbits, giving rise to low- and high-molecular-weight antibodies. Cross-reactivity experiments revealed the presence in LPS of both type-specific and group-reactive antigenic determinants.
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The atypical Bacteroides fragilis lipopolysaccharide is chemotactic for polymorphonuclear leukocytes. The chemotactic activity is mediated by complement activated through the alternative pathway. This mediator has been isolated. It is a polypeptide with a molecular weight of 16,000 and is most likely the complement component C5a.
This review deals mainly with the taxonomy of the genera and species in the family Bacteroidaceae. It has been proposed that the genus Bacteroides should be restricted to include the "Bacteroides fragilis group" and that the asaccharolytic black-pigmented Bacteroides species be transferred to a new genus, Porphyromonas. New Bacteroides, Fusobacterium, and Selenomonas species have been described. A high degree of heterogeneity is apparently present among Peptostreptococcus species. Mobiluncus is a novel genus including gram-variable curved rods isolated from the human vagina. Brachyspira has been proposed as the generic name for spirochetes isolated from patients with intestinal spirochetosis.