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T Hopf

Publications and source records attributed to T Hopf.

At least 19 recordsLinked to original sources
PubMed1999

Resulting tensile forces in the human bone-patellar tendon-bone graft: direct force measurement in vitro.

The objective of this study was to measure the resultant force in the human bone-patellar tendon-bone graft after reconstruction of the anterior cruciate ligament under various conditions in vitro. Seven fresh-frozen cadaver lower extremities were used. Force measurement was made with a quartz force transducer mounted in a specially designed load cell. The effect of passive extension movement, quadriceps pull, varus torque, and valgus torque on the resultant force in the ligament were investigated. Passive extension of the joint generated a rapid increase of force in the graft between 30 degrees and 0 degrees of flexion, reaching its maximum (128+/-25 N) at full extension. When quadriceps pull was applied to extend the joint, resultant force increased at 50 degrees of flexion and reached its maximum (219+/-25 N) at full extension. Additional resistance applied to the level of the ankle joint generated an additional load of the graft. Increase of forces in the ligament resulted from both varus and valgus applied moments.

Aged↗
PubMed1998

Ankle orthoses effect on single-limb standing balance in athletes with functional ankle instability.

OBJECTIVE: To test whether a rigid or a flexible ankle orthosis affects postural sway in single-limb stance as quantified by stabilometry. DESIGN: Crossover trial. SETTING: University laboratory. PARTICIPANTS: Twenty-two athletes with functional ankle instability (consecutive sample of patients with recurrent ankle sprains but without mechanical instability) and 22 healthy athletes (control group of volunteers matched to age, height, weight, physical activity). INTERVENTIONS: Stabilometry in single-limb stance on a force platform. Participants were tested on each leg with and without a rigid or a flexible ankle orthosis. The order of test conditions was randomized. MAIN OUTCOME MEASURES: Sway velocities, sway pattern, and sway area as calculated from center of pressure movements. The two groups were compared by Mann-Whitney test, and the different orthoses within each group were compared by Wilcoxon test, paired samples (type I error 5%, Bonferroni adjustment). RESULTS: In athletes with functional ankle instability, both a rigid and a flexible ankle orthosis significantly reduced mediolateral sway velocity. A flexible ankle orthosis also changed sway pattern significantly, by reducing the percentage of linear movements of less than 5 degrees per .01 sec. CONCLUSIONS: In athletes with functional ankle instability, ankle orthoses reduce mediolateral sway velocity, possibly because of improved mediolateral proprioception.

Adult↗
PubMed1998

Color Doppler imaging assessment of blood flow in vascularized pedicle grafts for avascular necrosis of the femoral head: angiographic correlation.

The aim of this study was to examine whether color Doppler imaging can be used to assess vascular patency after use of pedicle pelvic bone grafts in the operative treatment of avascular necrosis of the femoral head. We performed color Doppler imaging and selective angiography in 10 consecutive patients (12 hip joints) treated for avascular necrosis of the femoral head. In comparison to angiographic results, nine patent and two occluded grafts were demonstrated correctly by color Doppler imaging. In one case, a graft confirmed as patent by angiography was not demonstrable by color Doppler imaging. Accuracy, sensitivity, and specificity of color Doppler imaging were high when compared to angiography as a standard. Color Doppler imaging is suited to evaluate vascular patency after use of vascularized pedicle bone grafts in the treatment of avascular necrosis of the femoral head.

Angiography↗
PubMed1998

Invasion of human cartilage by cultured multicellular spheroids of rheumatoid synovial cells--a novel in vitro model system for rheumatoid arthritis.

OBJECTIVE: A new 3-dimensional cell culture system was established to examine the invasion of rheumatoid synovial cells into cartilage. METHODS: Synovial cells from 20 patients with rheumatoid arthritis (RA) and 15 patients with osteoarthritis (OA) were co-cultured as multicellular spheroids with cartilage fragments. RESULTS: After 4 weeks the rheumatoid spheroids eroded the cartilage. The destruction of cartilage was supported by a high expression level of cathepsin D and matrix metalloproteinases. In contrast, the OA tissue showed attachment to the cartilage only. CONCLUSION: Our results indicate that spheroid/cartilage co-cultures seem to be a suitable in vitro model for the study of destructive cellular mechanisms that occur in RA.

Arthritis, Rheumatoid↗
PubMed1997

[Fractures after total knee joint endoprosthesis. Results of treatment with various stabilizing methods].

In 20 patients with a fracture of femur or tibia 5.2 years after arthroplasty of knee the results of the operative treatment are presented. The results show that especially bone damaging diseases as rheumatoid arthritis, osteoporosis and the loosening of the endoprosthesis are favorable for the fracture during the follow-up. The conclusion of the investigation shows that in younger patients the external fixation by plates and screws is the preferential treatment, in elderly people or comminuted fracture an internal fixation, also in combination with an additional osteosynthesis, allows a fast mobilization. The number of observed complications is higher than in primary knee arthroplasty, the full weight bearing 1st delayed. The rate of further operations and unsatisfactory results is also higher being affected by the high mean age of the operated patients (73.4 years). The possible use of a total femur implant must be discussed critically because only an individual production can avoid further damage of the parts of the joint that were not concerned by the fracture.

Aged↗
PubMed1996

Chondropathia patellae and knee muscle control. An electromyographic study.

The activity of knee-related muscles was registered via exercising on a bicycle ergometer by 17 patients with clinically diagnosed chondropathia patellae. M. quadriceps activity was shorter and the hamstring activity longer in the chondropathy group compared with a matched healthy control group. The changes in m. quadriceps occurred to an almost equal extent in lateral and medial sections. In five patients with unilateral complaints, the electromyographic changes were nevertheless noted on both sides. The study shows that chondropathia patellae involves a change in muscle control affecting not only the knee extensors but also the hamstrings. Through the changed innervation pattern the coactivation phase, i.e. the phase of simultaneous activation of knee flexors and extensors at the end of the extension phase, takes place at a higher angle of flexion. Physiotherapy should involve all knee-related muscles and should include not only isometric but also dynamic exercises.

Adult↗
PubMed1996

Evidence for a selected humoral immune response encoded by VH4 family genes in the synovial membrane of a patient with rheumatoid arthritis (RA).

The analysis of rearranged antibody-encoding genes from B cell foci in rheumatoid synovial tissue has characterized these cells as highly mutated memory B cells with a high proportion of members of the VH4 family. In order to characterize further the VH4 response in one patient, B cell-rich areas from different sections of synovial membrane (SM) were identified by CD20 staining, isolated by microdissection and pooled, in order to analyse highly enriched B cells without selection by in vitro culture procedures. From DNA of about 5 x 10(3) B cells rearranged VH genes were amplified by polymerase chain reaction (PCR) and cloned. Sequencing of 11 clones containing rearranged VH4 gene products revealed that seven were potentially functional, and all were mutated with 84-96% homology to known germ-line (gl) genes and VH4 gl genes amplified from the patient's genomic DNA. Analysis of the complementarity determining region (CDR) 3 revealed that two products represented members of one B cell clone which differed by five nucleotide changes. Three of the five mutations encoded amino acid replacements in CDRs indicating antigen-driven expansion of one specific clone. Additional analyses of 25 members of three B cell clones from isolated aggregates showing intraclonal diversity in one of three clones provided further evidence that antigen selection takes place in the SM. Overall, the pattern of mutations and the replacement to silent (R:S) ratios were diverse, with six products indicating antigen selection by their high R:S ratios in CDRs. Although DNA analysis does not allow a characterization of antibody specificities, we can conclude from our analysis does not allow a characterization of antibody specificities, we can conclude from our analysis of antibody-encoding genes that selection by antigen and expansion of specific clones occur in the SM against the background of polyclonal activation.

Aged↗
PubMed1996

Constitutive expression of c-fos and c-jun, overexpression of ets-2, and reduced expression of metastasis suppressor gene nm23-H1 in rheumatoid arthritis.

OBJECTIVES: To identify genes that are involved in the development and progression of rheumatoid arthritis (RA). METHODS: We used a multiple gene analysis system and a set of available genes participating in processes such as proliferation, differentiation, tumour progression, and metastasis, to identify their RA related expression. Synovial tissues from 22 patients with RA were evaluated in comparison with those from six patients with osteoarthritis and two patients with non-inflamed joints as controls, using northern blot and reverse transcriptase polymerase chain reaction experiments. RESULTS: Our data confirm the role of c-fos and c-jun as constitutive signal transmitters in solid RA tissues, thus demonstrating the potential of the approach. Activation of both genes persisted through multiple passages of the cells in tissue cultures derived from the synovial lining of RA tissues. There was an increased expression of ets-2 in 30% of RA samples and an up to 30-fold decreased expression of the potential metastasis suppressor gene nm23-H1 in 90% of RA tissues, compared with control tissues. CONCLUSIONS: The data presented show for the first time a significant decrease of nm23-H1 expression in RA, which is possibly involved in local invasiveness, and a strong activation of the ets-2 nuclear oncogene in about one third of RA tissues, which may also be part of a pathway leading to advanced disease stages. The constitutive expression of c-fos and c-jun in RA tissue most probably results from a continuing inflammatory stimulus. These findings with cell cultures suggest an intrinsic activation mechanism of these early response genes in RA.

Arthritis, Rheumatoid↗
PubMed1996

[Treatment concepts of femoral fractures after total endoprosthetic replacement of the hip or knee joint: intra- or extramedullary stabilization?].

A comparison between intramedullary (n = 13) and extramedullary (n = 37) stabilization was performed in 50 patients (mean age 67.4 years) after fractures of the femoral shaft following knee or hip arthroplasty. Fracture of the femoral shaft (mean 5.5 years after the implantation) is observed especially often in patients with osteoporosis, loosening of the prosthesis, and rheumatoid arthritis. The ratio between cemented and uncemented endoprosthesis was 4:1. The investigation shows that extramedullary stabilization by means of plates and screws is the preferred treatment for younger patients and in the case of distal fractures, while in older people intramedullary fixation in combination with an additional osteosynthesis allows early mobilization. The number of complications observed is higher than with primary arthroplasty. The frequency of further operations, postoperative fractures and unsatisfactory results is significantly higher than in primary hip and knee surgery or uncomplicated fracture of the femur.

Adult↗
PubMed1995

The B lymphocyte in rheumatoid arthritis: analysis of rearranged V kappa genes from B cells infiltrating the synovial membrane.

The participation of the humoral immune system in rheumatoid arthritis (RA) is characterized by the production of rheumatoid factors (RF). RF are autoantibodies against the Fc part of IgG which are encoded by diverse germ-line genes. Most of the RF-encoding genes are unmutated, but in RA, a substantial quantity is encoded by somatically mutated genes. In addition, the synovial membranes (SM) of the diseased joints of RA patients are infiltrated by B lymphocytes which form germinal center-like aggregates. To analyze the local immune response, B cell foci from two RA SM were isolated by micromanipulation. From DNA of these foci, the rearranged kappa light chain variable region (V kappa) genes were amplified by polymerase chain reaction (PCR), cloned and sequenced. The amplification of different V kappa-J kappa combinations of different foci suggested oligoclonal expansion of B lymphocytes, which was confirmed by sequence analysis: each PCR product contained members of a single B cell clone. The sequence analysis of 29 different clones revealed rearrangements of diverse V kappa genes. Both frequent representatives of the V kappa 3 and the V kappa 1 family, as well as rarely used genes such as the L10 and B2 genes of the V kappa 2 and V kappa 5 families were found. Of the eleven potentially functional gene rearrangements, eight were significantly mutated, indicating their derivation from antigen-selected B cells. Intraclonal diversity in one of these clones may suggest ongoing mutation in the diseased synovial membrane of patients with RA.

Adult↗
PubMed1995

Changes in muscular activity after knee arthrotomy and arthroscopy.

The object of this study was to find out whether an arthrotomy of the knee would damage joint proprioception. Twenty patients who underwent arthrotomy, and 20 patients after arthroscopy, were tested on a modified ergometer and the electromyographic action of their quadriceps and hamstrings recorded. The quadriceps action started later and had a shorter duration (20 ms) after arthrotomy. The differences are statistically significant in intra-individual comparison both for vastus lateralis and medialis, in comparison with the normal group for only the vastus medialis. There were no significant differences in muscle control after arthroscopy. These results are in favor of preferring arthroscopy to arthrotomy whenever possible.

Adult↗
PubMed1995

Proliferation enhancement by spontaneous multiplication of chromosome 7 in rheumatic synovial cells in vitro.

Mosaic trisomy of chromosome 7 is known to occur in a variety of non-neoplastic hyperproliferative disorders. In long-term cell cultures established from rheumatic synovium with mosaic trisomy 7, we observed a continuous increase in the proportion of cells with trisomy 7 to over 50% by the 10th in vitro passage. Simultaneous in situ hybridization with a repetitive chromosome-7-specific DNA probe and fluorescent Ki-67 labelling showed a strong correlation between trisomy 7 and an elevated proliferation index in cultured rheumatic synovial cells. Moreover, we observed a fraction of rapidly proliferating cells with up to eight copies of chromosome 7 as the sole cytogenetic change. Frequent somatic pairing of centromeres of two chromosomes 7 in interphase nuclei suggests either atypical non-disjunction with a persisting centromere or selective endoreduplication of chromosome 7.

Arthritis, Rheumatoid↗
PubMed1995

Shoulder problems in high level swimmers--impingement, anterior instability, muscular imbalance?

The objective was to study prevalence and underlying pathology of "swimmer's shoulder". Twenty-two competitive swimmers of national "D-Kader" (elite development swimmers) were evaluated by means of questionnaire, clinical examination and isokinetic testing of external rotation and internal rotation. At the examination current interfering pain necessitating a cessation or reduction of practice was found in 5 (23%) athletes. At isokinetic testing 8 (36%) athletes complained of shoulder pain. Any history of pain was seen in 14 (64%) swimmers. A positive impingement sign was noted in 11 (50%) athletes. Apprehension sign which is indicative of anterior instability was found in 11 (50%) swimmers. Clinical equivalents of dysfunction of scapulothoracic muscles such as scapular winging (5 athletes) and shoulder protraction (12 athletes) were noted. For comparison of results of isokinetic testing a control group of non-swimmers was selected matching the group of swimmers exactly in terms of age, sex and dominant side. External rotation/internal rotation ratio of peak torque and total work at 60 deg/sec and 180 deg/sec was significantly lower in swimmers than in controls. The ratio was independent of sex, dominant side, history of pain and pain at examination. During internal rotation competitive swimmers produced significantly higher peak torques and total work than controls. There was no significant difference in external rotation. In conclusion there are several different abnormalities of function contributing to the pathology of "swimmer's shoulder":--Laxity of anterior-inferior capsuloligamentous structures with atruamatic anterior instability due to repetitive overload.--Impingement with rotator cuff tendinitis.--Muscular imbalance of the rotator cuff muscles and scapulothoracic dysfunction.

Adolescent↗
PubMed1995

[Clinical relevance of tunnel position and interference screw location after replacement-plasty of the anterior cruciate ligament with a patellar ligament transplant].

Fifty-eight patients had a prospective follow-up examination 1 year after arthroscopic ACL replacement. Stability measurement was done using a KT-1000 arthrometer. The side-to-side difference was less than 2 mm in 40 patients, 2-4 mm in 11 patients and more than 4 mm in 7 patients. The position of the tibial and femoral tunnels and interference screw was measured from the postoperative X-ray. The positions of screws and tunnels were highly variable. However, there was no statistical correlation between the position of the femoral interference screw and stability measurement or between the position of the femoral interference screw and flexion contracture or between the position of the tibial interference screw and the extension contracture. However, out of six patients with the femoral tunnel in an extremely anterior position (> 20 mm anterior of the over the top position), only one patient had a stable joint (< 2 mm difference in KT 1000 with 89 N anterior shear load) at follow-up. A screw divergence of more than 10 degrees was found in 18 patients. A tibial interference screw with more than 30 degrees of divergence was seen in seven patients. The primary fixation strength of the bone block could be diminished in these cases. This may have an impact on the early rehabilitation protocol.

Adolescent↗
PubMed1994

[Biomechanical principles of after-care in replacement of the anterior cruciate ligament].

The patellar tendon graft replacing the anterior cruciate ligament undergoes histomorphological and biomechanical changes. From literature we can estimate a force of 200 N to be a critical limit of graft load after ACL-plasty in humans. The comparison with the graft loads under various conditions provided the following conclusions: Unlimited passive motion of the knee joint can be done without risk. Early weight bearing is possible. Active extension exercise against gravity could damage the graft in the range of 0 to 20 degrees of flexion in some patients. A rehabilitation protocol has to regard these biomechanical principles. In a prospective study 74 patients underwent rehabilitation according to the protocol described. An extension contracture of more than 10 degrees was seen in 9 patients. In all cases it was due to a notch-impingement or a cyclops. One year after operation 57 patients had a follow up. The side to side-difference in anterior drawer measured with a KT-1000-arthrometer was less than 2 mm in 45 patients. Six patients had difference between 2 and 4 mm. In six patients the difference was more than 4 mm.

Adolescent↗
PubMed1994

[Arthrofibrosis after replacement-plasty of the anterior cruciate ligament--incidence, causes, therapy].

The objective of the study was to assess prospectively the percentage of patients with restricted range of motion (arthrofibrosis) after ACL-plasty and to assess the clinical benefit of arthroscopic surgery. We had arthrofibrosis in 9 out of 74 cases (12%). In 8 cases there was a combined lag of flexion and extension and in one case there was only a lag of extension. In all patients range of motion could be improved by arthroscopic surgery. There was a significant raise in extension (13.3 degrees) and in flexion (28.7 degrees). In the arthrofibrosis-group more patients had undergone surgery for acute ACL-rupture (66%) than in the group without arthrofibrosis (32%).

Adolescent↗
PubMed1994

[Intramedullary pressure in the femur during boring and nailing with modern compression interlocking nails--risk of fat embolism?].

Fat embolism syndrome occurs in only 0.9-4% of patients with long bone fractures and especially with intramedullary nailing. Earlier publications have shown that intramedullary manipulation, e.g. reaming and nailing, can produce high pressures of up to 1 bar. The design of the new non-slotted interlocking compressions nails seems to increase the pressure in the femoral cavity. We measured the intramedullary pressure during reaming of the marrow cavity and insertion of compression nails (OSTEO), using cadaver femora and a piezo pressure transducer. We simulated a proximal fracture and performed 30 drilling and 20 nailing procedures. On average we detected a maximum pressure of 0.26 bar during drilling and 0.63 bar during nailing. During reaming the pressure increased when the reamer had passed the narrow diaphysis and reached the metaphysis. When the tried to enlarge the femoral canal by pushing and pulling the reamer repeatedly we measured high pressure peaks. During nailing we detected short impulses lasting a few milliseconds. The results show that the new compression nail do not produce higher intramedullary pressure than slotted nails. It is possible to avoid a dangerous pressure level by using a careful operative technique.

Biomechanical Phenomena↗