PubMed Health⌕ Search

Biomedical subjects

T Huizinga

Publications and source records attributed to T Huizinga.

At least 19 recordsLinked to original sources
PubMed1999

Cytokine gene polymorphism in human disease: on-line databases.

The pathologies of many infectious, autoimmune and malignant diseases are influenced by the profiles of cytokine production in pro-inflammatory (TH1) and anti-inflammatory (TH2) T cells. Interindividual differences in cytokine profiles appear to be due, at least in part, to allelic polymorphism within regulatory regions of cytokine gene. Many studies have examined the relationship between cytokine gene polymorphism, cytokine gene expression in vitro, and the susceptibility to and clinical severity of diseases. A review of the findings of these studies is presented. An on-line version featuring appropriate updates is accessible from the World Wide Web site, http://www.pam.bris.ac.uk/services/GAI/cytokine4.htm.

Cytokines↗
PubMed1999

Microsatellite alleles and single nucleotide polymorphisms (SNP) combine to form four major haplotype families at the human interleukin-10 (IL-10) locus.

Interleukin-10 (IL-10) is a pivotal immunoregulatory cytokine, influencing many aspects of the immune response. The IL-10 gene is located on chromosome 1 at 1q31-32 and is highly polymorphic. One microsatellite and three single nucleotide polymorphisms (SNPs) have been recorded within the 1.2 kb immediately upstream of the gene, with an additional microsatellite present at 4 kb upstream. The relationship between these two classes of polymorphism is poorly defined in the IL-10 gene. Haplotypes have been presented comprising alleles from the two microsatellite loci, and independently from the three SNPs, but these have not yet been brought together to define unified halpotypes. In the present report we describe the 29 IL-10 haplotypes found in 56 Dutch European families and show that they fall into four major haplotype groups, each of which spans the 4 kb upstream of the IL-10 gene and has a different distribution of IL10.G alleles. In addition, we describe three novel single nucleotide polymorphisms in the human IL-10 gene and suggest how they relate to these four haplotype families.

Alleles↗
PubMed1979

Solution rate of crystals at fluid-fluid interface.

A model system was developed in which the dissolution behavior of a single crystal of potassium ferricyanide was studied at a liquid paraffin-water interface. Since the equilibrium position of a crystal at the interface is independent of its size, the lifetime of a crystal dissolving at the interface is determined entirely by its initial size and its dissolution rate in the water phase. The dimensions of every crystal were measured microscopically before dissolution. A continuous-flow recording dissolution apparatus was used to measure spectrophotometrically the mass flow of dissolved potassium ferricyanide. The dissolution cell in this system was mounted in an optical bench, making it possible to follow dissolving crystals visually by projecting them on a screen. The results show that the lifetime of a crystal is proportional to the shortest length of the crystal face in contact with the liquid paraffin and is rather independent of its form. Furthermore, crystal shape changes during dissolution, which is explained partly by the nonisometric dissolution of potassium ferricyanide crystal faces and partly by the nonconstancy of film thickness.

Chemistry, Pharmaceutical↗