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T Humphrey

Publications and source records attributed to T Humphrey.

35 records · Page 2Linked to original sources
PubMed1987

Cleavage and polyadenylation of messenger RNA precursors in vitro occurs within large and specific 3' processing complexes.

We have investigated the assembly of complexes associated with in vitro cleavage and polyadenylation of synthetic pre-mRNAs by native gel electrophoresis. Incubation of SP6-generated pre-mRNA containing the adenovirus L3 polyadenylation site in HeLa cell nuclear extract results in the rapid assembly of specific complexes. Formation of these complexes precedes the appearance of cleaved intermediates and polyadenylated products and is dependent on an intact polyadenylation signal within the pre-mRNA. The specific complexes do not form on RNAs with point mutations in the AAUAAA sequence upstream of the L3 polyadenylation site. Furthermore, such mutant RNAs cannot compete for factors involved in the assembly of specific complexes on wild-type pre-mRNA. Upon complex formation a 67-nucleotide region of the L3 pre-mRNA is protected from RNase T1 digestion. This region contains both the upstream AAUAAA signal and the GU-rich downstream sequences. Cleavage and polyadenylation occur within the specific complexes and the processed RNA is subsequently released. We propose that the assembly of specific complexes represents an essential step during pre-mRNA 3' end formation in vitro.

Adenoviruses, Human↗
PubMed1983

Development and implementation of a music/activity therapy intake assessment for psychiatric patients. Part I: initial standardization procedures on data from university students.

The stimulus for the Music/Activity Therapy Intake Assessment was a recent publication by the Joint Commission on Accreditation of Hospitals requiring for activity services, "assessment of the patient's needs, interests, life experiences, capacities, and deficiencies" (1981, p. 126). The Assessment was written in the spring of 1982 and contains four sections: Activity Preference, Organizational involvement, Attitude Survey, and Post-Interview Observations. The purpose of the present study was to investigate the psychometric properties of the Attitude Survey. The Attitude Survey was administered to 214 university students enrolled in three areas of study: human science, performance, and liberal arts. Results show that each of the three scales in the Survey is internally consistent (.63 to .74), that each scale measures a single large dimension, and that two of the three scales significantly discriminate between groups of students (p less than .05). The authors conclude that the Attitude Survey possesses adequate psychometric properties to justify its continued use as part of the Music/Activity Intake Assessment.

Attitude to Health↗
PubMed1980

The effect of music ear training upon the auditory discrimination abilities of trainable mentally retarded adolescents.

The purpose of this study was to investigate the effect of music ear training upon the auditory discrimination abilities of 30 institutionalized trainable mentally retarded (TMR) adolescents. Subjects were 15 TMRs enrolled in choir and 15 TMRs who were not choir members. The hypothesis was that music ear training incorporated in choir rehearsals would enhance auditory discrimination abilities, as measured by the Goldman-Fristoe-Woodcock Test of Auditory Discrimination. An analysis of variance revealed significantly more errors in auditory discrimination by the TMR adolescents not enrolled in choir. Results of t tests comparing the scores of TMR choir members to Goldman-Fristoe-Woodcock test norms for the same chronological age revealed significantly more errors by the choir members. However, t tests comparing the performance of TMR choir members and Goldman-Fristoe-Woodcock test norms for normal subjects of the same mental age revealed significantly more errors by normal subjects.

Adolescent↗