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Biomedical subjects

T I Sergeeva

Publications and source records attributed to T I Sergeeva.

At least 19 recordsLinked to original sources
PubMed1992

[Effect of regional (wound) revaccination with tetanus anatoxin on the functional activity of immunocompetent cells in the region].

The study made with the use of complex methods established that the local (wound) application of tetanus toxoid rapidly made the manifestation of the lysosomal apparatus more pronounced, increased the oxidizing activity (determined in the nitro blue tetrazolium test) and phagocytic activity of the mononuclear phagocytizing system in the wound and in the regional lymph nodes. The wound application of tetanus toxoid significantly increased blast transformation of T lymphocytes in guinea pigs simultaneously with tetanus wound infection. The study confirmed the pathogenetic expediency of the proposed method for the stimulation of anti-tetanus immunity by the application of tetanus toxoid on the wound which specifically inhibited the primary stage of the infectious process.

Administration, Topical↗
PubMed1992

[Eremomycin in the treatment of antibiotic-associated colitis in golden hamsters].

The efficacy of eremomycin, a new glycopeptide antibiotic, was studied on a model of antibiotic-associated colitis in golden hamsters. The colitis was induced by intraperitoneal or intragastric administration of lincomycin. In a dose of 100 mg/kg administered orally once a day for 5 days eremomycin protected the animals from the lincomycin-induced colitis: some animals survived, the others died in later periods. When the animals were infected with a pathogenetic strain of Clostridium difficile followed by exposure to lincomycin the use of eremomycin produced the similar effect.

Administration, Oral↗
PubMed1991

[In vitro activity of a new glycopeptide antibiotic eremomycin in relation to obligate anaerobic Gram-positive bacteria].

Antibacterial activity of eremomycin, a novel glycopeptide antibiotic, against obligate anaerobic Gram-positive++ bacteria was studied. Eremomycin was shown to inhibit the growth of obligate anaerobic Gram-positive++ cocci and bacteria belonging to Clostridium in rather low concentrations and within narrow ranges of the MIC which was indicative of the antibiotic undoubted advantages. The antibacterial activity of eremomycin was 2 times as high as that of vancomycin and 8 times as high as that of ristomycin with respect to Gram-positive++ anaerobic cocci. Pathogenic strains of Clostridium spp. were 2 to 4 times more sensitive to eremomycin than to vancomycin. A significant property of the novel glycopeptide antibiotic was shown to be its capacity for inhibiting the growth of Gram-positive++ aerobic and obligate anaerobic cocci within the same concentration ranges which might be of importance in monotherapy of mixed aerobic and anaerobic infections.

Anti-Bacterial Agents↗
PubMed1990

[Sensitivity of the main causative agents of gas gangrene to currently available antibacterial drugs].

Sensitivity of the main microbial species causing gas gangrene to 21 antibacterial drugs was studied. The pathogens included 28 strains of Clostridium perfringens, 21 strains of C. oedematiens, 31 strains of C. septicum and 19 strains of C. histolyticum. Antibiotics having a bactericidal effect on the Clostridia were determined. The findings of the study were indicative of a variety of the antibiotic sensitivity spectra of the Clostridium spp., which accentuated the importance of antibiotic sensitivity assay in every particular case of gas gangrene.

Anti-Bacterial Agents↗
PubMed1990

[The isolation of the ribosomal fraction of Clostridium perfringens type A and an evaluation of its protective activity].

A method for isolation of the ribosomal fraction (RF) from the cytoplasm of type-A C. perfringens strain BP6K was developed and its chemical and antigenic properties characterized. RF has been found to possess protective properties: two subcutaneous immunizations of mice with RF preparations adsorbed on Al(OH)3 in doses of 250 and 500 micrograms (dry weight) has ensured, on the average, the protection of 41.9% of the immunized animals from 1 DCL of type-A C. perfringens strain BP6K culture.

Animals↗
PubMed1989

[Immunomorphologic aspects of local (wound) revaccination with tetanus anatoxin].

The immunomorphological reaction of regional lymphoid organs, the pathomorphology of wound tissues, humoral antitoxic response and a protective effect after local (wound) booster immunization with tetanus toxoid have been studied in observations on 100 guinea pigs with experimental wound infection. The study has shown that the local application of tetanus toxoid, besides stimulating humoral response, induces a more rapid effect aimed at the primary elements of the infectious process (the germination of spores, the adhesion, colonization and toxin formation of the causative agent), thus facilitating the localization of the focus of infection, the development of reparative processes in the wound and the arresting of the infection.

Animals↗
PubMed1989

[Preparation of diagnostic antitoxic serum to Clostridium difficile].

The results of the studies on the preparation of diagnostic antitoxic sera to C. difficile, intended for use in biological assays with the aim of the laboratory diagnosis of clostridial enteric infections, are presented. The conditions for the detoxification of C. difficile native toxin have been established, the optimum schedules for the immunization of rabbits have been selected and specific antitoxic sera to C. difficile have been obtained. The neutralizing activity of these sera has been evaluated in the lethality test and in the cytotoxic test on human embryo dermo-muscular fibroblast cells M-19.

Animals↗
PubMed1987

[Excretion of 5-hydroxyindole-3-acetic and 5-methoxyindole-3-acetic acids in cancer patients].

Daily urinary excretion of 5-hydroxy-3-indoleacetic acid (5-HIAA) and 5-methoxyindole-3-acetic acid (5-MIAA) was studied by chromatography-mass-spectrometry in patients with cancer and healthy subjects. A considerable increase in urinary 5-MIAA-excretion was shown in patients with cancer of the stomach, rectum and lung. In some cases, a correlation was found between high urine levels of carcinogenic 5-MIAA and similarly high levels of 5-HIAA excretion with urine.

Humans↗
PubMed1986

[Antigenic interrelations within the species Clostridium novyi, C. histolyticum and C. septicum as a basis for developing an immunofluorescence method].

The cellular and antigenic interrelations within each Clostridium species have been studied with a view to the selection of strains for the preparation of specific rabbit antisera possessing a wide serological spectrum of action within the species. The indirect immunofluorescence test making it possible to determine the species of clostridia in native pathological material from wounds or in cultures, subjected to short-term incubation, within 1-1.5 hours has been developed. This test can be used in laboratory practice for the diagnosis of gas gangrene in humans, as well as for the identification of pathogenic clostridia.

Animals↗
PubMed1986

[Methodological approaches to the rapid determination of the sensitivity of the causative agent of gas gangrene to antibiotics].

The results of the studies on development of a rapid assay of antibiotic sensitivity of gas gangrene causative agents with their high nutrient requirements under obligatory anaerobiosis are summarized. The data on development of a uniform standard nutrient medium with the use of dry components made in the USSR are presented for the first time. The medium is intended for rapid assay of antibiotic sensitivity of the main causative agents of gas gangrene, i.e. C. perfringens, C. oedematiens, C. histolyticum and C. septicum.

Anti-Bacterial Agents↗
PubMed1985

[Blastomogenic activity of biogenic methoxyindoles].

A considerable blastogenic activity of biogenic methoxyindoles--melatonin, 5-methoxytryptamine (5-MOT) and their common metabolite--5-methoxyindolyl-3-acetic acid (5-MIAA) was established by prolonged s.c. injection in C57BL/6 mice. However, the blastogenic effect of 5-MOT decreased by 26% when its further metabolism to 5-MIAA was blocked for some time and the synthesis of its carcinogenic metabolite was inhibited. These results showed that the blastogenic effect of 5-MOT is not direct; and it is mediated by its transformation to 5-MIAA in the body.

5-Methoxytryptamine↗
PubMed1985

[Carcinogenic action of 5-methoxytryptamine related to its transformation into 5-methoxyindolyl-3-acetic acid].

A considerable blastomogenic effect of metabolite serotonin 5-methoxytryptamine (5-MOT) subcutaneously administered for a long time to C57BL/6 mice was established. This effect was decreased noticeably if the further metabolism of 5-MOT into 5-methoxyindolyl-3-acetic acid (5-MIAA) was blocked by pyrazidol. These results explain the fact that the blastomogenic effect of 5-MOT is not direct but is caused by the transformation of 5-MOT into its final carcinogenic metabolite 5-MIAA.

5-Methoxytryptamine↗
PubMed1984

[Morphological and histochemical study of the action of a native Clostridium perfringens type A enterotoxin on a model of the ligated ileal loop in the rabbit].

Morphological studies carried out in the igated loop of the rabbit small intestine, used as a model, have revealed that the action of the enterotoxins of C. perfringens type A reference strain and C. perfringens type A strains isolated in the USSR on the intestinal mucosa is absolutely identical. This action is manifested by the destruction of epithelial cells on the tops of the villi, inflammatory infiltration and considerable exudation of fluid into the lumen of the intestinal loop. Early lesions of the brush border of enterocytes, accompanied by the loss of alkaline phosphatase activity and the disappearance of the glycocalix, have-been histochemically established. C. perfrinegens antienterotoxic serum has proved to be capable of completely neutralizing the histopathological activity of C. perfringens enterotoxin.

Alkaline Phosphatase↗