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T I Tikhonenko

Publications and source records attributed to T I Tikhonenko.

At least 127 records · Page 7Linked to original sources
PubMedDate not supplied

[Fragmentation of the DNA of simian adenovirus type 38 by using restriction endonucleases].

The effect of restricting endonucleases Eco R I, BgI II and Sal I on simian adenovirus type 38 (SV-38) DNA was studied. Bgl II restrictase cleaves the virus DNA into 4 fragments, A, B, G, and D, with molecular weights 9.3 x 10(6), 3.3 x 10(6), and 2.9 x 10(6) daltons, respectively. After restriction with Eco R I and Sal I SV-38 DNA cleaves into 5 and 6 fragments, respectively. The molecular weights of Eco R I fragments are A--8.2 x 10(6), B--6.5 x 10(6), C--4.0 x 10(6), D--1.27 x 10(6), and of Sal I fragments: A--6.5 x 10(6), B--5.4 x 10(6), C--4.2 x 10(6), D--2.8 x 10(6), E--2.5 x 10(6), and F--0.25 x 10(6). By restriction of DNA-protein compex by means of partial DNA hydrolysis, combined Bgl II and Eco R I hydrolysis and secondary restriction of a fragment eluted from the agar gel, the alternation of Eco R I and Bgl II fragments in SV-38 genome was determined.

Adenoviridae↗
PubMedDate not supplied

[Simian adenovirus type 7 (SA7) DNA fragmentation by specific R.Bgl II and R.Hind III endonucleases].

The effect of restricting R. BgIII and R. HindIII endonucleases on the genome of simian adenovirus type 7 (SA7) was studied. The R. BgIII endonuclease has 5 restriction sites on SA7 DNA and produces 6 fragments with molecular weights of 10.0 X 10(6), 5,3 X 10(6), 3.6 X 10(6), 1.8 X 10(6), 0.6 X 10(6), and 0.5 X 10(6) daltons. R. HindIII, having 3 restriction sites, hydrolyses SA7 DNA into 4 fragments with molecular weights of 7.6 X 10(6), 5.9 X 10(6), 5,2 X 10(6), 3,1 X 10(6) daltons. The R. BgIII and R. HindIII fragments are arranged in the SA7 genome in the following order: B-A-C-E-D-F and C-D-B-A.

Adenoviridae↗