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Biomedical subjects

T Ikuta

Publications and source records attributed to T Ikuta.

At least 91 records · Page 5Linked to original sources
PubMed1983

Purification of C-reactive protein.

A concise method was designed for preparation of C-reactive protein (CRP) from pleural effusion. By addition of L-alpha-lecithin to the pleural effusion in the presence of calcium ions, a flocculence of the CRP-lecithin complex formed. Subsequent treatment of the CRP-lecithin complex with chloroform and sodium citrate buffer enabled extraction of the CRP in the buffer layer. This extracted CRP was further purified by sequential treatment on column chromatography of DEAE cellulose (DE52) and gel filtration using Sephacryl S-300. The isolated protein was proved to be native CRP with a high degree of purity, as determined by electrophoretical and immunological analysis. The yield was 41.8% recovery from the starting material. E1%(280) of the CRP preparation was estimated to be 18.75.

Animals↗
PubMed1982

Sex differences in the human group mean SEP.

The following results were obtained with SEPs recorded from strictly age-matched normal human subjects, 100 males and 100 females, 22.29 +/- 1.90 years. (1) The sex factor on the wave form of SEP was confirmed highly significant by MANOVA. (2) It was confirmed that the group mean SEP of each sex each converges to a given wave form which significantly differed from each other. (3) The differences in the wave form of the group mean SEP between sexes were established. The group mean SEP for females consisted of the 12 components, N1, P1, N2, P2, N3, P3, N4, P4, N5, P5, N6 and P6, and was approximately tetraphasic within 500 msec in latency, while that for males consisted of the same components except for N4 and P4. The baseline amplitude of the wave form of the group mean SEP, smaller in males than in females, differed significantly between sexes, corresponding to the most remarkable difference in its wave form, in the descending slope from the highest positive peak P2 to the deep negative trough N6, where there was a high convex arch with P4 (80 msec) in females, a low hollow slope without the peak in males. The latencies of the components tended to be longer in males than in females. (4) With scaled SEPs, which were converted from SEPs by amplitude scaling, results were similar to those with SEPs, and the sex difference in the scaled group mean SEP was also verified to be significant. These results indicate the further possibility of data reduction by amplitude scaling.

Adult↗
PubMed1981

Purification and immunological determination of alpha 2-macroglobulin in serum from injured rats.

Rat alpha 2-macroglobulin was isolated and purified from the pooled sera of turpentine-injected rats by sequential use of dextran sulphate, DEAE-cellulose and gel filtration chromatography. The final protein product obtained by this procedure proved to be alpha 2-macroglobulin of a high degree of purity based on electrophoretic, immunologic and centrifugal analysis. The alpha 2-macroglobulin preparation also binds stoichiometrically to trypsin preventing subsequent inhibition by protein trypsin inhibitors. SDS-polyacrylamide gel electrophoresis of rat alpha 2-macroglobulin after incubation with trypsin suggested that there are at least two susceptible peptide bonds in the 170,000-dalton alpha 2-macroglobulin subunit. The concentration of alpha 2-macroglobulin in the sera of rats was measured by electroimmuno assay a monospecific antiserum against alpha 2-macroglobulin. Purified alpha 2-macroglobulin was used as a standard. Sera from normal male rats contained 32 +/- 4 micrograms of alpha 2-macroglobulin per ml. To determine the time course of response of alpha 2-macroglobulin to inflammation, rats were subjected to either laparotomy or subcutaneous injection of turpentine. After either type of injury, the concentration of alpha 2-macroglobulin increased rapidly, reaching a maximum value of 110-140 times that of the control value by 24 h. Little difference was noted in responsiveness between the two sexes.

Animals↗
PubMed1981

The waveforms of the group mean SEP of each sex.

With SEPs recorded from age-matched normal human subjects, 100 males and 100 females 22.34 + 1.69 years, it was confirmed that the Group Mean SEP of each sex each converges to a given waveform which significantly differed from each other. The waveform of the Group Mean SEP for females consisted of the 12 components, and was approximately tetraphasic within 500 msec in latency, while that for males consisted of the same components except for N4 and P4. There was a high convex arch with P4 (82 msec) between P2 and N6 for females, but a low hollow slope without the peak for males. With Scaled SEPs, which were converted from SEPs by Amplitude Scaling, the results similar to those with SEPs were obtained.

Adult↗
PubMed1981

Differences in human group Mean SEP between sexes: with reference to statures.

With 200 SEPs of normal human subjects, the differences in the Group Mean SEP between sexes were defined, removing those attributable to the peripheral factor, the statures. The differences in baseline amplitude in the sections roughly 1) between 29-110 msec, where there were the most remarkable differences in the waveform of the Group Mean SEP, between P2 and N6, with a high convex arch peaking at P4 (80 msec) for females but not for males, 2) around 320 msec, and 3) behind 400 msec in latency, were verified, not attributable to the different statures, and might be the central origin. With Scaled SEPs, which were converted from SEPs by the Amplitude Scaling, results similar to those with the unscaled SEPs were obtained.

Body Weight↗
PubMed1981

Differences in human group mean SEP between sexes: with reference to the Rohrer's index.

With 200 SEPs of normal subjects, the differences in the Group Mean SEP between sexes were defined eliminating the differences attributable to another peripheral factor relating to the nutritional condition represented by the Rohrer's index. The differences in the baseline amplitude of the Group Mean SEP between males vs. females in the section between roughly 23-111 msec, around 330 msec, and behind 389 msec in latency were verified, subtracting the differences between the groups with a high vs. low value of the Rohrer's index, not attributable to the different nutritional condition, and might be the central origin. Applying the Amplitude Scaling, the differences in the section 23-104 msec in latency were verified similarly, being more a significant indication of the sex difference per se.

Adolescent↗
PubMed1981

Sex differences in the human group mean SEP.

With 200 SEPs of normal human subjects, the sex differences in the waveform of the Group Mean SEP were established eliminating those attributable to the peripheral factors, the statures and the nutritional conditions represented by Rohrer's index. The differences in baseline amplitude of the Group Mean SEP between sexes, in the sections between 25--110 msec and behind 400 msec in latency, were verified removing those between the tallers and the shorters and those between with high and with low values of Rohrer's index, not attributable to these peripheral factors and might be the central origin. The differences in the Scaled Group Mean SEP were also verified similarly, being more a significant indication of the sex difference itself than those in the Group Mean SEP.

Body Height↗
PubMed1980

The waveform of the group mean SEP of normal human subjects.

The following conclusions were deduced from 200 SEPs recorded from 100 male and 100 female controls, of average age 22.34 +/- 1.70 years. (1) It was confirmed that the waveforms of a group mean SEP and of a group SD converge to a waveform which is not a flat horizontal line. (2) The waveform of the group mean SEP for the 200 normal subjects consisted of 12 components and was tetraphasic within 500 msec latency. The waveform of the group SD for the 200 normal subjects was also shown. (3) Some coincidences were established in early components within 50 msec latency, between the group mean SEP obtained in the present study and the group schematic SEPs reported by others, but large discrepancies were noted in later components. (4) With scaled SEPs, which were converted from unscaled SEPs by amplitude scaling in order to eliminate individual variation in absolute amplitude, similar results were obtained to those with unscaled SEPs. These results indicate the possibility of data reduction by amplitude scaling.

Adult↗
PubMed1980

The group mean SEP of normal human subjects.

The following conclusions were deduced from the SEPs of 100 normal human subjects, whose mean age was 21.80+/-1.62. It was confirmed that the waveform of a Group Mean SEP and of a Group SD each converges to a waveform different from a flat horizontal line. The waveform of the Group Mean SEP consisted of 12 components, and was roughly tetraphasic within 500 msec in latency. Large discrepancies were found in the later components between the Group Mean SEP and the Group Schematic SEPs reported by others, although some coincidences were shown in the early components within 100 msec in latency. The results derived from the Scaled SEPs, which were converted from the (unscaled) SEPs, indicated the possibility of a reduction of the data by the Amplitude Scaling.

Adult↗