Biomedical subjects

T Inoué

Publications and source records attributed to T Inoué.

3 recordsLinked to original sources

PubMed1992

The interface between epithelium and lamina propria in the rat urinary bladder.

We investigated by transmission and scanning election microscopy the interface between the epithelium and lamina propria in the rat urinary bladder. A digestion technique that dissolves the basal laminae and collagen fibrils was effective in cleaving the mucosa at this level; the specimens were then prepared for scanning electron microscopy, thus visualizing the basal epithelial surface and the uppermost surface of the lamina propria. The underside of the epithelium is scored by very numerous grooves which in the intact organ are occupied by a dense network of blood capillaries. These vascular grooves allow a large number of capillaries (epithelial capillaries) to run at a distance of a few tenths of a micron from the epithelium. On the side of the lamina propria, after collagen and other extracellular materials had been removed, the capillary network itself is visible in the uppermost region. The network is complementary to that of vascular grooves. Other smaller grooves on the basal surface of the epithelium correspond to nerve fibres which run within a few tenths of a micron from the epithelium.

Animals↗

PubMed1992

Complementary scanning electron microscopy: technical notes and applications.

This report introduces practical techniques and applications of complementary scanning electron microscopy (SEM). To identify the complementary structures at high magnification, we first made a montage pair of low magnification micrographs as a guide map. Consulting the map, we took complementary micrographs at high magnification. When taking a picture at high magnification, we drew the outline of the most prominent structures on a transparent plastic plate attached to the cathode-ray tube of a SEM. Then another picture of the complementary structures was taken after adjusting the complementary structures to the reverse image of the plastic plate. We performed three applications of the complementary SEM: 1) complementary observation of the epithelial underside and lamina propria of the rat urinary bladder; 2) complementary observation of the fractured Golgi apparatus; and 3) evaluation of specimen drying methods. After proper digestion of the rat urinary bladder using strong alkali, the epithelium was detached from the underlying lamina propria. On the basal side of the epithelium, the meshwork of grooves were visible. The observation of the corresponding lamina propria confirmed that the grooves were occupied by blood capillaries located in the uppermost part of the lamina propria. The complementary observation of the Golgi apparatus was useful for understanding its three-dimensional architecture. The observation on both complementary fractured surfaces of the Golgi apparatus from the mouse lacrimal gland demonstrated the continuity of the Golgi stacks. It was also effective for observing both the cis and trans side of the same Golgi stack. Complementary SEM was also useful for evaluating the specimen drying method.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

PubMed1991

Scanning electron microscopic observations on muscle cells of experimental mitochondrial myopathy produced by 2, 4-dinitrophenol.

The morphological changes of the skeletal muscle cells of the rat experimental myopathy induced by 2, 4-dinitrophenol were examined by scanning electron microscopy in comparison with the ultrastructure of normal muscle cells. Specimens were prepared by the Aldehyde-Osmium-DMSO-Osmium method which permits the three-dimensional demonstration of intracellular structures under SEM. In the specimen prepared by the method, myofibrils having been completely dissolved, intracellular membranous structures such as the sarcoplasmic reticulum, T-tubules and mitochondria were clearly demonstrated in three dimensions. In the experimental mitochondrial myopathy, large accumulations of mitochondria were observed at the subsarcolemmal region. Mitochondria in the perinuclear and intermyofibrillar region showed swelling and occasionally accompanied abnormal concentric cristae. The sarcoplasmic reticulum which showed regular network in normal muscle cells entirely disappeared in the mitochondrial myopathy. Although the mitochondrial changes obtained in this study were almost identical to those previously reported by transmission electron microscopy, the changes in the sarcoplasmic reticulum have not been described in previous works.

2,4-Dinitrophenol↗