Biomedical subjects

T Irino

Publications and source records attributed to T Irino.

At least 19 recordsLinked to original sources

PubMed2001

Multiscale computing.

Multiscale computing (MSC) involves the computation, manipulation, and analysis of information at different resolution levels. Widespread use of MSC algorithms and the discovery of important relationships between different approaches to implementation were catalyzed, in part, by the recent interest in wavelets. We present two examples that demonstrate how MSC can help scientists understand complex data. The first is from acoustical signal processing and the second is from computer graphics.

Computer Graphics↗

PubMed2001

A compressive gammachirp auditory filter for both physiological and psychophysical data.

A gammachirp auditory filter was developed by Irino and Patterson [J. Acoust. Soc. Am. 101, 412-419 (1997)] to provide a level-dependent version of the linear, gammatone auditory filter, with which to explain the level-dependent changes in cochlear filtering observed in psychophysical masking experiments. In this 'analytical' gammachirp filter, the chirp varied with level and there was no explicit representation of the change in filter gain or compression with level. Subsequently, Carney et al. [J. Acoust. Soc. Am. 105, 2384-2391 (1999)] reviewed Carney and Yin's [J. Neurophysiol. 60, 1653-1677 (1988)] reverse-correlation (revcor) data and showed that the frequency glide of the chirp does not vary with level in their data. In this article, the architecture of the analytical gammachirp is reviewed with respect to cochlear physiology and a new form of gammachirp filter is described in which the magnitude response, the gain, and the compression vary with level but the chirp does not. This new 'compressive' gammachirp filter is used to fit the level-dependent revcor data reported by Carney et al. (1999) and the level-dependent masking data reported by Rosen and Baker [Hear. Res. 73, 231-243 (1994)].

Auditory Perception↗

PubMed2000

Specific gel electrophoresis method detects two isoforms of human intestinal alkaline phosphatase.

We have demonstrated that the 6.0% polyacrylamide disc gel electrophoresis (PAGE) method in the presence of 1% Triton X-100 clearly separated both normal molecular mass intestinal alkaline phosphatase (NIAP) and bone alkaline phosphatase (BAP) in serum regardless of the ABO blood group and the secretor status of the subjects. From the results under the usual 7.5% PAGE condition, overlapping mobilities of NIAP and BAP were found in particular in nonsecretor subjects after a high-fat meal. Under the above conditions, the apparent BAP percentage three hours after a meal was higher in nonsecretors than in subjects under fasting conditions, because NIAP activity in serum rose sharply following a high-fat meal. In contrast, under our 6.0% PAGE method, the NIAP and BAP were clearly separated from each other regardless of whether the subjects were fasting or had ingested a high-fat meal. In addition, an elevated level of the circulating NIAP can be another marker for patients with liver cirrhosis. Considering all these factors, the 6.0% PAGE method proposed by us is not only a useful method for the separation of intestinal alkaline phosphatase (IAP) isoforms, but can also be useful for the analysis of other usual AP isozymes.

Adolescent↗

PubMed1999

Reduction of reaction differences between human mercaptalbumin and human nonmercaptalbumin measured by the bromcresol purple method.

Although the bromcresol purple (BCP) method provides high specificity in measurements of serum albumin concentrations, we discovered a reaction difference between the values for human mercaptalbumin (HMA) and human nonmercaptalbumin (HNA) measured by the BCP. We found that the color intensity of HMA with BCP present in the reduced form in the albumin of fresh serum, was lower than for HNA. While maintaining specificity for albumin, we reduced the reaction difference between HMA and HNA, with the addition of sodium dodecylsulfate and 5,5'-dithiobis(2-nitrobenzoic acid) to the BCP reagent. The mean albumin concentration by the BCP procedure for 63 fresh sera and the 63 sera stored for 2 days at room temperature were 35.6 g/L and 38.1 g/L, respectively. Those by the modified BCP procedure were 39.8 g/L and 39.9 g/L, respectively. The difference in measured values between the fresh and stored sera which is believed to be caused by the conversion of HMA to HNA during the storage of sera was not observed in the case of the modified BCP procedure. Our modified BCP method is effective in eliminating uncertainty of the albumin concentration assigned to assay calibrators for the conventional BCP method.

Absorption↗

PubMed1998

Changes in intestinal alkaline phosphatase isoforms in healthy subjects bearing the blood group secretor and non-secretor.

We found the high molecular mass intestinal alkaline phosphatase (HIAP) and normal molecular mass intestinal alkaline phosphatase (NIAP) in serum at fasting and after fatty meal by use of 6.0% polyacrylamide gel electrophoresis (PAGE) in the presence of 1% Triton X-100. HIAP only appeared in serum of Lewis blood group secretors ¿Le(a-b+)¿, and HIAP levels were dependent on ABO blood groups. Among the secretors, the highest activities of HIAP in fasting serum were observed in subjects with blood groups O and B (8.6+/-1.4 U/1; mean+/-SD) and the lowest activities were associated with blood group A (0.7+/-0.2 U/1; mean+/-SD), and the HIAP activities did not change after fatty meal. In contrast, NIAP was present in the serum of both secretors and non-secretors regardless of ABO blood group. Trace amounts of NIAP remained in fasting serum; however serum NIAP activities rose sharply after fatty meal. The remaining ratios of NIAP activity at fasting and 9 h after fatty meal of secretors were approximately the same as those of non-secretors. The electrophoretic mobility on PAGE or the apparent molecular mass estimated by gel filtration of serum NIAP in secretors was slightly different from that in non-secretors. In addition, HIAP can be normalized to NIAP on PAGE in the absence of Triton X-100, and the electrophoretic mobility of normalized-NIAP was identical to that of original NIAP in secretors. Accordingly, it can be concluded that the structure of serum NIAP in the secretor was different from that in the non-secretor, because HIAP is only formed by serum NIAP in the secretor. These results suggest that differences in serum NIAP in the secretor and the non-secretor may be closely related to the appearance of IAP in the circulation.

ABO Blood-Group System↗

PubMed1998

Pharmacokinetics and pharmacodynamics of FK143, a nonsteroidal inhibitor of steroid 5 alpha-reductase, in healthy volunteers.

The pharmacokinetics and pharmacodynamics of FK143, a new nonsteroidal inhibitor of steroid 5 alpha-reductase, were investigated in healthy volunteers, with use of plasma FK143 concentrations and serum dihydrotestosterone levels as an index for pharmacologic effects. The area under the plasma concentration-time curve from zero to infinity [AUC(0-infinity)] and maximum plasma concentration [Cmax] were increased dose proportionally after oral administration (100 to 500 mg) while subjects were in the fed state. The AUC(0-infinity) and Cmax after 500 mg oral administration during fed conditions were significantly larger than those during the fasted state, suggesting an increase of the absorption of FK143. Dihydrotestosterone concentrations after a single administration of FK143 (100 to 500 mg) during fed conditions decreased to about 65% of predose values and thereafter slowly recovered to the same levels as predose values at 168 hours. A combined pharmacokinetic-pharmacodynamic model was constructed with use of changes in dihydrotestosterone concentrations. The pharmacokinetic-pharmacodynamic profiles of FK143 after repeated administration were predictable with use of the pharmacokinetic-pharmacodynamic parameters obtained after a single administration of FK143.

5-alpha Reductase Inhibitors↗

PubMed1998

Modeling temporal asymmetry in the auditory system.

Sound sources in the environment produce waves that are almost invariably asymmetric in time, and human listeners are highly sensitive to temporal asymmetry. The spectral analysis and neural transduction processes in the cochlea enhance temporal asymmetry, as do time-domain models of cochlear processes, but it appears that the resulting asymmetry is not sufficient to explain the observed perceptual asymmetry. In the auditory image model (AIM) of hearing, the temporal asymmetry in the neural activity produced by the cochlea is further enhanced by the "strobed" temporal integration that converts the neural activity pattern into an auditory image, and the temporal asymmetry in the auditory image is sufficient to explain the perceptual asymmetry. Modern versions of the "duplex model" of pitch have time-domain cochlea simulations that produce neural activity with temporal asymmetry similar to that produced by AIM. In the final stage, however, they apply autocorrelation to the neural pattern and autocorrelation is a symmetric process in time. In this paper the effect of autocorrelation on temporal asymmetry is examined in a range of auditory models with varying forms of auditory filterbank, compression, and neural transduction. It is concluded that autocorrelation does not enhance temporal asymmetry and often reduces it, and that autocorrelogram models cannot explain the magnitude of the perceptual asymmetry in their current form. Then, the original version of strobed-temporal-integration is reviewed with regard to temporal asymmetry, and the delta-gamma theory of temporal asymmetry [Irino and Patterson, J. Acoust. Soc. Am. 99, 2316-2331 (1996)] is used to develop a new version of strobed-temporal-integration that is more robust and physiologically more plausible.

Auditory Perception↗

PubMed1998

Properties of high-molecular-mass placental alkaline phosphatases in normal pregnancy sera.

We examined the appearance of high-molecular-mass placental alkaline phosphatases (HPLAPs) in the serum of normal pregnant women by means of polyacrylamide gel electrophoresis (PAGE) in the presence of Triton X-100. The HPLAPs were undetectable or only slightly detectable by PAGE in the absence of Triton X-100. The HPLAPs were detected in all sera sampled during the last trimester of pregnancy. The catalytic activities of total placental alkaline phosphatase (TPLAP) and HPLAPs were correlated (r = 0.96) and the ratio of HPLAPs/TPLAP catalytic activity was 0.20 (0.06) (mean and SD) in 40 serum samples from pregnant women. The HPLAPs appear to be formed from a common dimeric placental alkaline phosphatase (PLAP) (common-PLAP), as judged by the fact that they were formed again after removal of HPLAPs from serum by gel filtration. The formation of HPLAPs was more prominently observed with the faster fractions of gel filtration. The apparent molecular mass of the HPLAPs in pregnancy serum was 720 KDa by gel filtration. HPLAPs were not converted to common-PLAP by phosphatidylinositol-specific phospholipase (PIPL) C and PIPL-D treatments. The HPLAPs were selectively incorporated into liposomes consisting of phosphatidylcholine/cholesterol, and most of the PIPL-D-treated PLAP could from HPLAPs, while a small amount of PLAP could not form HPLAPs. On the other hand, HPLAPs in pregnant women's sera and HPLAPs prepared from partially purified PLAP in vitro could be converted to common-PLAP by brief treatment with subtilisin. However, the highly purified PLAP could not form HPLAPs in the presence of Triton X-100. These results suggest that PIPL-D-resistant and PLAP-associated serum protein may regulate the conversion of PLAP to HPLAP in the presence of Triton X-100.

Alkaline Phosphatase↗

PubMed1996

Temporal asymmetry in the auditory system.

When a damped exponential with a half-life of 4-8 ms is repeated every 25-50 ms and used to modulate a sinusoid or a wideband noise, it suppresses the sound quality typically associated with the carrier. When the envelopes of these "damped" sounds are reversed in time, producing "ramped" sounds, a continuous component with the sound quality of the carrier is restored to the perception. This paper presents an experiment that measures the temporal asymmetry revealed by this perceptual contrast. A ramped sinusoid or noise with a given half-life was presented with a damped sinusoid or noise having the same or greater half-life, to determine the damped half-life required to produce a continuous component with the equivalent relative strength in the two sounds. The results with sinusoidal carriers show that the half-life of the damped sound has to be, on average, about five times the half-life of the ramped sound if the tonal component of the two perceptions is to have the same relative strength. The asymmetry for the noise carrier is about half that of the sinusoidal carrier and, again, the damped sound has the greater matching half-life. Several multichannel auditory models based on a gammatone filterbank are used to try to explain the data in terms of traditional leaky integration, but they produce neither sufficient asymmetry nor the correct pattern of asymmetry. A "delta-gamma" theory is then developed to provide a framework for understanding temporal asymmetry in the auditory system. The theory is used to compare the temporal asymmetry produced by several auditory models and to explain when and how they can accommodate the perceptual asymmetry observed in the experiments.

Auditory Perception↗

PubMed1996

[Non-specific neurological complaints and silent cerebral infarction].

To clarify the relationship between non-specific neurological complaints and silent cerebral infarction (SCI), we studied 82 patients (56 +/- 8 years old; mean +/- SD) who presented with at least one complaint (headache, dizziness, forgetfulness) and 76 normal volunteers with no complaints (55 +/- 7 years old). All subjects were evaluated with a questionnaire for complaints and with 0.5 T magnetic resonance imaging of the brain for the presence of SCI. Several risk factors for stroke were also studied. SCI was significantly more common in the patients (18%) than in the normal subjects (7%, p < 0.05). SCI was more common in subjects with dizziness (40%) and in those with headache (18%) than in those with no complaints (p < 0.01, p < 0.05, respectively). Dizziness and forgetfulness were closely associated with SCI (p < 0.05). Two risk factors for stroke, age and hypertension, correlated with SCI. These results clearly show that non-specific neurological complaints are closely related to SCI.

Aged↗

PubMed1994

Phosphorylcholine as a unique substrate for human intestinal alkaline phosphatase.

1. The enzymatic nature of human liver, bone, placental and intestinal alkaline phosphatases (ALPs) were investigated with phosphorylcholine (PC), phosphorylethanolamine, pyridoxal-5'-phosphate and p-nitrophenylphosphate at a weakly alkaline pH. 2. The apparent Km value of the intestinal ALP with PC was the highest of all ALPs tested. Intestinal ALP hydrolyzes PC the most and has higher affinity for choline as a transphosphorylating acceptor than the other ALPs. In addition, the intestinal ALP activity with PC was most susceptible to Na2HPO4, in the tested ALPs. 3. The present results suggest that PC is a unique substrate for human intestinal ALP, which may be related to the metabolism of PC or choline as part of phosphatidylcholine.

Alkaline Phosphatase↗

PubMed1993

Determination of proteins by a reverse biuret method combined with the copper-bathocuproine chelate reaction.

A method of protein determination has been developed which combines the biuret reaction and the copper(I)-bathocuproine chelate reaction. Protein in the specimen forms a Cu(2+)-protein chelate complex (biuret reaction) during the first step. Excess Cu2+ is reduced to Cu+ by ascrobic acid, allowing the Cu+ to form a Cu(+)-bathocuproine chelate complex during the second step. The amount of Cu(+)-bathocuproine chelate complex formed is inversely proportional to the protein concentration. The sensitivity (epsilon = 1.4 x 10(6) 1.mol-1.cm-1 against human albumin) of this method was higher than that of the original Lowry (9.8 x 10(5)), pyrogallol red (1.0 x 10(6)) and commercially available Coomassie Brilliant Blue G.250 methods (6.7 x 10(5)). The color intensities of human gamma-globulin, human globulin (fractions IV-1 and IV-4), bovine albumin, egg albumin and horse gamma-globulin against human albumin (100%) ranged from 92 to 101%. The results obtained with the present method (y) correlated well with those determined by the biuret method (r = 0.998, y = 0.98 chi - 0.002, x = 1.31, y = 1.29 g/l) in 30 diluted sera. These results confirm that this assay is similar in sensitivity to the original Lowry method, is rapid and has similar reactivity to each of the various proteins in biological fluids.

Ascorbic Acid↗

PubMed1992

[Spontaneous cleft palate in CF#1/Ohu mice].

We found a spontaneous cleft palate in a mouse of CF#1/Ohu (Ohu University, Japan). Further, the frequency of the spontaneous cleft palate in strains of CF#1/Jms (Institute of Medical Science, University of Tokyo, Japan) and CF#1/Jah (National Institute of Animal Health, Japan) were about 3%, respectively. The frequency and the types of spontaneous cleft palate in CF#1 were clearly different from those in A/J and CL/Fr strains which were used as model animals of lip and cleft palate. We think that CF#1 is the new animal models to analyze a cleft palate genetically and biochemically.

Animals↗

PubMed1991