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Biomedical subjects

T Iu Salina

Publications and source records attributed to T Iu Salina.

7 recordsLinked to original sources
PubMed2004

[Production of gamma-interferon by blood mononuclear cells in patients with various types of a tuberculous process].

To elucidate the diagnostic value of gamma-interferon (gamma-IFN) in the human body, the gamma-IFN production that was spontaneous and induced by BCG Mycobacterium bovis in the supernatants of short-term cultures of peripheral mononuclear cell from 31 patients with active tuberculosis was studied. The sandwich method of enzyme immunoassay was used, by employing two monoclonal antibodies in the avidin-biotin system. The studies have established the low spontaneous production of gamma-IFN in patients with the severe progressive course of a tuberculous process (22.5 +/- 2.5 pg/ml in Group 1 versus 41.5 +/- 8.6 pg/ml in patients with the favorable course of a tuberculous process (Group 2) and 34.0 +/- 3.5 pg/ml in healthy individuals (Group 3); p(1-2) < 0.05 and p(1-3) < 0.05)). Moreover, there was a reduction in the induced production of gamma-IFH in response to BGC (37.7 +/- 33.4 pg/ml in Group 1 versus 214.9 +/- 81.9 pg/ml in Group 2 and 206.0 +/- 31.1 pg/ml in healthy individuals; p < 0.05 and p(1-3) < 0.001), which is of diagnostic and prognostic values.

Adult↗
PubMed2004

[Interferon-gamma and IgG antibodies to M. tuberculosis in the serum of patients with active pulmonary tuberculosis].

The above parameters in the sera from patients with active tuberculosis (n = 20) and healthy individuals (n = 16) were studied in parallel to examine the relationship and mutual effects of interferon-gamma (IFN-gamma) and IgG antibodies to Mycobacterium tuberculosis, as well as their relationship with some clinical and immunological features of tuberculous infection. The relative and absolute counts of CD4 and CD16 cells were additionally measured in these patients' peripheral blood. Patients with active tuberculosis were found to have significantly elevated serum levels of IFN-gamma, IgG antibodies and CD16 cells with the decreased count of CD4 cells as compared with the similar parameters in healthy individuals. There was no relationship of the serum level of IFN-gamma to the clinical features of tuberculosis. The serum level of IFN-gamma is likely to determine the interferonogenic activity of the causative agent itself.

Adult↗
PubMed2003

[Enzyme immunoassay and immunochromatographic assay in the differential diagnosis of tuberculosis and cancer of the respiratory organs].

The informative value of immunodiagnostic assays aimed at identifying specific antibodies in the peripheral blood was studied. Indirect solid-phase enzyme immunoassay (Antitub test system, Moscow) and rapid immunochromatographic assay (TB-CHECK, France) were used. Fifty-six diagnostic patients and 18 healthy individuals were examined. The diagnosis of active tuberculosis was verified in 29 patients (Group 1) and that of lung cancer was in 27 patients (Group 2). The detection rate of and the level of antituberculosis antibodies in patients with active tuberculosis greatly differed from those in patients with lung cancer (p < 0.01). In the diagnostic patients, the sensitivity of enzyme immunoassay was 79.3%, its specificity was 88.9; the sensitivity and specificity of the rapid assay were 72.4 and 96.3%, respectively. The high specificity of the immunochromatographic assay in patients with lung cancer may reduce the number of false-positive results of the enzyme immunoassay. The concurrent use of both methods is an essential adjunct to the clinical examination of diagnostic patients, it makes it possible to reduce the time of pre-examination of cancer patients and to restrict a group of patients who need for invasive studies to specify a diagnosis.

Adult↗
PubMed2003

[Some clinical and immunological aspects of female pulmonary tuberculosis].

Expression of activation markers, such as CD25, HLA-DR), Fas-AG, CD16), on the lymphocytes was studied in female patients with tuberculosis (n = 40) and healthy donors. The level of antituberculous antibodies was measured in parallel in the sera of these patients. The above parameters were examined in relation to the nature of the microflora detected in these patients. Nonspecific co-infection in patients with active tuberculosis is an additional factor of activation of the immune system and simultaneously a factor that enhances the involvement of cells in apoptosis.

Adolescent↗
PubMed2000

[Comparative study of various diagnostic techniques efficiency for tuberculosis].

Thirty-one patients with active pulmonary tuberculosis were examined. The efficiencies of modified luminescence microscopy (with superfine iron added), enzyme immunoassay (using two test systems made in Moscow and Saint Petersburg (Russia) and the MycoDot rapid diagnosis (USA) were compared. The highest sensitivity was found in the modified luminescence microscopy (45.2%) and enzyme immunoassay [Moscow test system (48.4%)]. The enzyme immunoassay (Saint Petersburg test system) and Mycodot were noted for its less sensitivity, but its high specificity.

Adolescent↗
PubMed1998

[Immunotherapy of pulmonary tuberculosis].

The efficiency of treating patients with destructive tuberculosis by using different immune correctors was comparatively analyzed. Five hundred and fifty three patients were treated with thymalin, thymogen, human leukocytic interferon, and a combination of thymogen and interferon. The benefit from immunocorrector treatment was obtained early in the complex therapy and the best result was achieved by the use of thymogen plus interferon. However, the action of the two immunocorrectors increased when fibrous deformity of the lung was healed, followed by the development of pronounced residual changes.

Adjuvants, Immunologic↗