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Biomedical subjects

T Iwadare

Publications and source records attributed to T Iwadare.

4 recordsLinked to original sources
PubMed1995

Staining etched epoxy resin sections for light microscopy.

Staining of etched sections for light microscopy is described. Azan staining was successful after treatment with potassium dichromate and the use of concentrated dye solutions. To remove osmium for hematoxylin-eosin staining, removal by reduction with ferrocene was used instead of oxidation. Highly selective differentiation after hematoxylin staining was achieved using p-toluenesulfonic acid-DMSO. To enhance eosin staining, a 2-bromoethylamine link between eosin and the tissue was used. Ferrocene also facilitated counterstaining of nuclei with hematoxylin after the PAS reaction. Periodic acid-methenamine silver staining was carried out without modification.

Epoxy Resins↗
PubMed1990

A solution for removal of resin from epoxy sections.

Development of a resin-dissolving solution for use at low alkali concentrations is described. Crown ether dissolved in dimethyl-sulfoxide produces a superbasic alkoxide anion. A five minute treatment resulted in complete resin removal from kidney biopsy specimens embedded in Epon 812. Specimens were well stained by Loeffler's methylene blue. Periodic acid-methenamine silver and Giemsa stains yielded good results. Application of PAS reaction and subsequent hematoxylin counterstaining was practicable for diagnosis.

Alkalies↗
PubMed1984

Dimensional changes of tissues in the course of processing.

Dimensional changes of bovine kidney and liver tissues in the course of processing under four different schedules were measured. Shrinkage of tissues at dehydration, substitution with intermedium and paraffin infiltration stages was 1-2%, 2-5% and 5-10% respectively. There was no significant difference in final dimension and morphology among the specimens processed under the different schedules. Any one of the investigated schedules can be employed for practical tissue processing.

Animals↗