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Biomedical subjects

T J Albert

Publications and source records attributed to T J Albert.

At least 19 recordsLinked to original sources
PubMed1998

Use of cervicothoracic junction pedicle screws for reconstruction of complex cervical spine pathology.

STUDY DESIGN: A retrospective review of 21 patients in which cervical pedicle screw fixation was used at C7 with or without upper thoracic pedicle screw fixation. OBJECTIVE: To evaluate the use of pedicle screw placement in the lower cervical spine. SUMMARY OF BACKGROUND DATA: The use of posterior cervical spine fixation, including lateral mass fixation, has become increasingly popular in recent years. However, lateral mass fixation at C7 is often hindered by lack of substantial high quality bone. The end level of long cervical spine constructs is frequently C7 or T1. Dissatisfaction with lateral mass fixation at C7 and T1 led the authors to use lower cervical pedicle screw fixation for several cervical spine disorders. METHODS: Twenty-one patients who had undergone cervical pedicle screw fixation at C7 were reviewed retrospectively. There were 12 males and 9 females, with an average age of 52 years. All pedicle screws were placed, after direct palpation of the pedicle, with a right angle nerve hook after laminoforaminotomy at C7. RESULTS: There were no neurologic complications related to pedicle screw placement, and no patient was symptomatically worse after the operation. Six patients with root pathology improved. Of 14 patients with cervical myelopathy, 12 improved at least one Nurick grade, and 2 had no improvement. There were no failures of fixation or complications related to pedicle fixation at a minimum of 1 year follow-up. CONCLUSION: Pedicle screws in C7 placed with laminoforaminotomy and palpation technique appears to be safe and efficacious. Excellent fixation can be achieved.

Bone Screws↗
PubMed1998

Variation in surgical opinion regarding management of selected cervical spine injuries. A preliminary study.

STUDY DESIGN: The opinions of orthopedic surgeons and neurosurgeons were compared regarding appropriate management of selected cervical injuries and the timing of stabilization. OBJECTIVE: To determine whether there is consistency of opinion regarding the management of cervical trauma. SUMMARY OF BACKGROUND DATA: Numerous forms of management for cervical trauma exist, but there are few consistent recommendations. No previous study has been done to determine uniformity of preferences of the surgeons who manage these injuries. METHODS: Thirty-one orthopedic surgeons and neurosurgeons were given a brief clinical situation and pertinent radiographic studies of five selected cervical injuries. Management options included halo and nonhalo orthoses, traction, and various forms of anterior and/or posterior procedures. The surgeons rated, in whole numbers from 1 to 10, their opinions on the appropriateness of each technique. Each surgeon was given a case of a "generic" cervical injury, in which stabilization was required and for which preoperative alignment was adequate in traction. They gave opinions on the timing of stabilization, with a choice of four time frames. Four neurologic situations were rated, ranging from intact to complete cord injury. RESULTS: Of 46 possible responses to the five test cases regarding appropriateness, 18 ranged from 1 to 10, the largest possible variation. Only 2 had a range of 5 or less, implying better consensus among tested surgeons. Mean values ranged from 1.9 to 9.5. Agreementamong respondents regarding appropriateness was slight with a range of kappa statistics from 0.09 to 0.14. Of 16 possible responses regarding timing, 14 were within a range of 8 or higher. Within 24 to 72 hours was the generally preferred time frame, with all possible responses showing a range of 3 or 4. Results of a multiple analysis of variance showed no significant differences among respondents. CONCLUSION: There is a large variety of opinion regarding appropriateness of specific operative and nonoperative management procedures and surgical timing among the surgeons polled who manage cervical trauma. This implies that there is no widely accepted standard management procedure for many of these injuries.

Adolescent↗
PubMed1998

Long-term evaluation of vertebral artery injuries following cervical spine trauma using magnetic resonance angiography.

STUDY DESIGN: A prospective study to determine the long-term outcome of traumatically induced vertebral artery injuries. Magnetic resonance angiography was performed at the time of cervical injury and at a follow-up office visit. OBJECTIVE: To determine the long-term outcome in terms of arterial flow competency of traumatically induced vertebral artery injuries. SUMMARY OF BACKGROUND DATA: Vertebral artery injury associated with cervical spine trauma has been well documented; however its healing or nonhealing potential has not been elucidated. METHODS: During the 7-month period from July 1993 to January 1994, all patients admitted to the authors' institution with cervical spine injuries underwent magnetic resonance imaging and magnetic resonance angiography of the cervical spine to determine the patency of their vertebral arteries. Magnetic resonance angiography was performed at the time of injury and at a follow-up office visit. Twelve of 61 patients were found to have a lack of signal flow within one of their vertebral vessels during this study period. RESULTS: Eighty-three percent of the patients (five of six) who were available for follow-up observation in this study did not manifest flow reconstitution of their vertebral arteries after an average 25.8-month follow-up period. CONCLUSIONS: According to these data, most patients with vertebral artery injuries after nonpenetrating cervical spine trauma do not reconstitute flow in the injured vertebral arteries. This lack of flow must be considered if future surgery in this region of the cervical spine is contemplated.

Adult↗
PubMed1998

Magnetic resonance imaging analysis of lumbar disc changes below scoliosis fusions. A prospective study.

STUDY DESIGN: The authors of this prospective study examined the preoperative and 3-year postoperative magnetic resonance images of 14 patients undergoing anterior and posterior fusion and/or posterior fusion only for scoliosis. All magnetic resonance images were ready by two independent neuroradiologists, who were blinded to the purposes of the study, for the presence of disc narrowing, signal decrease on T2, or herniated nucleus pulposus before and after surgery. Particular attention was paid to the disc changes at the level directly below the end vertebral level of the fusion and two levels below the fusion in the lumbosacral spine existing before surgical intervention. OBJECTIVES: To evaluate the potential for disc degeneration distal to long scoliosis fusions with end fusion levels in the mid to lower lumbar spine. SUMMARY OF BACKGROUND DATA: The determination of end levels of fusion for contructs presently used to manage adult scoliotic deformity has been evaluated in terms of correction of curvature and late decompensation in coronal and sagittal plane balance after fusion. However, the natural history of the caudal, free-motion segments in terms of degeneration and/or correlation with pain has not yet been addressed. METHODS: Fourteen patients undergoing scoliosis fusion underwent magnetic resonance imaging before surgery and approximately 3 years after surgery. The scans were reviewed by two independent neuroradiologists who looked at three degenerative indices at the disc below the area of scoliosis fusion. The authors analyzed rates of change of the three degenerative indices in the pre- and postoperative magnetic resonance images and created associations between the observed changes on the magnetic resonance images and the clinical outcomes of pain, the presence or absence of solid fusion, and the need for repeat surgery. RESULTS: Estimates of the rates of change of the three degenerative indices one or two levels below the fusion were as follow: the chance of disc narrowing, .2-34%; the chance of a decreasing signal on T2, 5-54%, with a 23% incidence among this group; and the chance of herniated nucleus pulposus, 0-34%. There was a significant correlation between the presence of back and/or leg pain and the signal decrease one level below the fusion (P = .04). CONCLUSIONS: If these results are corroborated in a larger sample size, surgeons who manage deformity may have to consider altering fusion levels at the time of fusion based on magnetic resonance imaging predictors. The present data may help to inform patients about the risk of developing junctional degenerative changes and potential symptoms from these changes below scoliosis fusions.

Adolescent↗
PubMed1998

The benefits of early decompression in cervical spinal cord injury.

Decompression and stabilization have been shown to improve neurologic outcome in cases of cervical spine trauma with proven compression of the spinal cord. This paper reviews experimental and clinical research to clarify the benefits of early surgery for cervical spinal cord injury. The direct clinical benefit of early surgery is a theoretic improvement in neurologic recovery over that of delayed surgery. Additional benefits of early surgery include the clinical advantages of a decreased length of hospitalization and its associated complications and a decreased time to rehabilitation and mobilization. Proper, timely surgical intervention can better the physiologic environment so as to allow for maximum neurologic improvement.

Animals↗
PubMed1998

Neurologic deterioration after cervical spinal cord injury.

Neurologic deterioration after cervical spinal cord injury (SCI) at a regional spinal cord center was examined. This study examined the incidence of neurologic deterioration as well as associated risk factors in our patient population. Up to 5.8% of cervical SCI patients have been noted to deteriorate neurologically after admission. Risk factors have been early surgery, halo application, traction, and Stryker frame rotation. All cervical SCI patients admitted between 1978 and 1993 who had neurologic deterioration were studied for characteristics of their event, operative status, risk factors, mortality, and neurologic return at 1 year postinjury. Patients were divided into minor and major groups based on the degree of neurologic loss. Nineteen of 1,031 patients were identified as neurologically deteriorated (1.84%). There were 8 major and 11 minor group patients. The average time from injury to deterioration was 3.95 days. Of 10 patients undergoing surgery at < or =5 days, 8 deteriorated postoperatively. Potential risk factors were ankylosing spondylitis (three patients), sepsis (four patients), and intubation (four patients). Neurologic recovery at 1 year showed that 11 of 12 patients were improved. Neurologic deterioration occurred in 1.84% of our patients. Deteriorations were associated with surgery at <5 days after injury, ankylosing spondylitis, sepsis, and intubation.

Adult↗
PubMed1998

Accuracy of SPECT scanning in diagnosing pseudoarthrosis: a prospective study.

The present study attempted to analyze the efficacy of single photon emission computed tomography (SPECT) in diagnosing pseudoarthrosis after fusion using surgical exploration as the gold standard. This study examined the SPECT scans of 38 patients before they underwent surgical exploration of their fusion mass for suspected pseudoarthrosis or in conjunction with instrumentation removal. Surgical findings were compared with the radiologists' findings to determine the efficacy of SPECT in diagnosing pseudoarthrosis. Radiographic determination of pseudoarthrosis has been difficult after attempted fusion of the spine. Multiple radiographic modalities have been touted as accurate depicters of the failure of spinal fusion. However, no method has been found to be highly accurate in the clinical setting. Thirty-eight patients (mean age = 42.8, 21 males/17 females, 35 of 38 with instrumentation) underwent SPECT scans before surgical exploration of their fusion mass for suspected pseudoarthrosis or in conjunction with instrumentation removal as part of this prospective study. The average interval from their fusion procedure until their SPECT scan was 23.9 months (range, 9-120 months). All surgical findings were recorded with regard to solidity of the fusion and the level of the possible pseudoarthrosis. All SPECT scans were read at a time after surgery by an independent nuclear radiologist who had not read their SPECT scans before surgery and who did not know the results of exploration. Results of the radiologist's reading were then compared with surgical exploration findings, and sensitivity and specificity was calculated. There were 24 solid fusions and 14 pseudoarthroses. SPECT scans correctly identified 7 of the 14 pseudoarthroses and 14 of the 24 solid fusions. This represents a sensitivity of 0.50 and a specificity of 0.58. SPECT scanning correctly diagnosed the one solid fusion and two pseudoarthrosis patients in the three patients who had no instrumentation. This study demonstrates that SPECT scanning alone is inaccurate in diagnosing pseudoarthrosis when using surgical exploration as the gold standard. Given recent pressures for cost containment, we cannot recommend SPECT scanning as a routine modality for use in the diagnosis of pseudoarthrosis. We cannot define the accuracy of SPECT scanning used together with computed tomography scans, plain films, or other radiographic modalities in the diagnosis of pseudoarthrosis.

Adult↗
PubMed1997

Vertebral artery occlusion associated with cervical spine trauma. A prospective analysis.

STUDY DESIGN: Radiographs and charts of 61 patients sustaining cervical spine trauma were studied prospectively to determine the incidence of vertebral artery injuries and possible correlative factors. Statistical analysis was conducted using chi-square testing of a two-way classification system. OBJECTIVES: To elucidate the incidence of vertebral artery injuries associated with cervical spine trauma, and to determine the value of various factors in predicting the existence of a vertebral artery injury. SUMMARY OF BACKGROUND DATA: During a 7-month period, 61 patients (41 male patients, 20 female; average age, 40.3 years) with cervical spine trauma were studied. METHODS: All patients admitted to the authors' hospital with cervical spine injuries underwent magnetic resonance imaging and magnetic resonance angiography of their cervical spine. All magnetic resonance angiographies were examined for vertebral artery injury. Data on demographics and the injury were recorded. RESULTS: Complete disruption of blood flow through the vertebral artery was demonstrated by magnetic resonance angiography in 12 of the 61 patients (19.7%). Ten of the 12 patients (83%) had either flexion distraction or flexion compression injuries. Age, sex, mechanism of injury, neurologic impairment, and associated injuries were not statistically significant in predicting the presence of a vertebral vessel occlusion. CONCLUSION: The findings in this study may support the need for vertebral vessel evaluation in selective patients, particularly those with flexion injuries and with neurologic symptoms consistent with vertebral artery insufficiency syndrome that do not correlate with the presenting bone and soft-tissue injuries.

Adolescent↗
PubMed1997

Steroids and gunshot wounds to the spine.

OBJECTIVE: The second National Acute Spinal Cord Injury Study demonstrated that there were neurological benefits from "spinal cord injury" doses of methylprednisolone for blunt spinal cord injuries. In this review, we examined the relative risk/benefit ratio of intravenously treating spinal gunshot wound victims with steroids. METHODS: A retrospective review was conducted of 254 consecutive patients who were treated between 1979 and 1994 for gunshot wounds to the spine (C1-L1) and a spinal cord injury. Three subgroups were established based on the administration of the steroids methylprednisolone (National Acute Spinal Cord Injury Study 2 protocol), dexamethasone (initial dose, 10-100 mg), and no steroids. All patients who received steroids were initially treated at another hospital and then transferred. No patients received steroids at our institution. The data analyzed included neurological outcome and infectious and noninfectious complications. RESULTS: No statistically significant neurological benefits were demonstrable from the use of steroids (methylprednisolone, dexamethasone). Infectious complications were increased in both groups receiving steroids (not statistically significant). Gastrointestinal complications were significantly increased in the dexamethasone group (P = 0.021), and pancreatitis was significantly increased in the methylprednisolone group (P = 0.040). The mean duration of follow-up was 56.3 months. CONCLUSION: In this retrospective, nonrandomized review, no neurological benefits were detectable from intravenously administered steroids after a gunshot wound to the spine. Both infectious and noninfectious complication rates were higher in the groups receiving steroids. Patients who sustain a spinal cord injury secondary to a gunshot wound to the spine should not be treated with steroids until the efficacy of such treatment is proven in a controlled study.

Adolescent↗
PubMed1997

An in vivo analysis of the dimensional changes of the neuroforamen after anterior cervical diskectomy and fusion: a radiologic investigation.

Eighteen patients (11 men, 7 women; average age, 45.5 years) who underwent anterior cervical diskectomy and fusion (ACDF) for the treatment of radiculopathy had preoperative and immediate postoperative computed tomography (CT) scans to measure pre- and postoperative foraminal heights and foraminal areas, preoperative disk space height, and postoperative graft height. The mean foraminal height preoperatively was 0.851 cm; postoperatively, it was 1.01 cm, with a mean percentage increase of 20% (-8.8 to 56.8%). Mean preoperative foraminal area was 37.53 mm2, increasing to a mean of 49.04 mm2 postoperatively with a mean percentage increase of 33% (range, -1.5 to 76.9%). No significant correlations between graft height and change in maximal foraminal height or foraminal area or between changes in foraminal height or area and postoperative symptom relief were found. Although significant increases in foraminal dimensions were seen radiographically after ACDF, these increases were variable and not strongly related to graft height. In addition, the increases in foraminal dimensions were not related to the short-term clinical results of ACDF. This study fails to support the hypothesis that the reliable results of ACDF can be ascribed primarily to indirect decompression of the uncovertebral foramen by disk-space distraction.

Adult↗
PubMed1997

Relationship of total hip arthroplasty outcomes to other orthopaedic procedures.

The Medical Outcomes Study Short Form-36 was used preoperatively and 2 years postoperatively to compare patients' self reported assessment of health and function between 151 patients who had primary total hip replacement and 49 patients who had total hip revision, 149 patients who had primary total knee replacements, 41 patients who had lumbar laminectomy, and 43 patients who had scoliosis surgery. Primary total hip arthroplasty and lumbar laminectomy posted equivalent followup scores. Primary total hip arthroplasty showed significant improvements in physical function and health perception when compared with revision total hip arthroplasty; all other health parameters were similar. Primary total hip arthroplasty showed significantly better followup scores and greater improvement in scores in four of nine categories of the SF-36 when compared with primary total knee arthroplasty (despite identical scores preoperatively). Despite a higher level of assessed health preoperatively, patients who had scoliosis surgery compared least favorably with patients who had primary total hip arthroplasty at 2 years followup. In terms of patient self assessment of health and function, primary total hip arthroplasty and lumbar laminectomy for radiculopathy gave the best results.

Adult↗
PubMed1996

Surgical anatomy of the vertebral arteries.

STUDY DESIGN: This study compared direct measurements of the distances between the vertebral arteries in the cervical spines of human cadaver specimens with data obtained from axial computed tomography images of these specimens. OBJECTIVE: To determine whether the information obtained from a computed tomography scan can be used reliably to predict the true anatomic location of the vertebral arteries and, in so doing, provide accurate guidelines for the lateral extent of anterior cervical decompressive procedures. SUMMARY OF BACKGROUND DATA: Iatrogenic vertebral artery injury during anterior cervical surgery is uncommon, potentially catastrophic, and avoidable. METHODS: The means and standard deviation of measurements of the location of the cervical segment of the vertebral arteries obtained with high-precision, digital calipers by direct gross anatomic dissection of 16 adult (eight male, eight female) cadaver specimens were recorded. These measurements were compared with computed tomography scan data obtained on the same specimens. RESULTS: The mean distances between the vertebral arteries progressively increased from C3 to C6. Computed tomography scan measurements of the distance between the cervical foramina transversaria were consistently smaller than direct measurements of the gross specimens. At C6, the computed tomography scan data were significantly less than the gross anatomic data. CONCLUSIONS: According to these data, computed tomography scan measurements may be used safely and accurately to plan the lateral extent of anterior cervical decompressive surgical procedures. Although the data obtained from the gross anatomic dissections may serve as guidelines to assist the surgeon, the authors recommend a careful review of the preoperative computed tomography scan on an individual case-by-case basis as the safest method to plan for anterior cervical surgery.

Anatomy, Cross-Sectional↗
PubMed1996

Health outcome assessment before and after lumbar laminectomy for radiculopathy.

STUDY DESIGN: Forty-one patients undergoing lumbar laminectomy for radiculopathy resulting from herniated discs assessed their health status using a generic health outcome instrument (Medical Outcomes Study Short Form 36) before surgery and at an average of 2 years after surgery. OBJECTIVES: To assess whether lumbar laminectomy for herniated nucleus pulposus is a useful intervention when patients evaluate their own perception of health. SUMMARY OF BACKGROUND DATA: The medical Outcomes Study Short Form 36 has been used in multiple studies assessing various medical conditions. It is brief, generic, and reliable. Although surgical treatment for radiculopathy by lumbar laminectomy has been shown to be successful using specific criteria for patient selection and an algorithmic approach, the authors are not aware of any study using a patient-based health outcome assessment to evaluate the results of this type of surgery. METHODS: Forty-one patients (82% completed follow-up evaluation; average follow-up period, 2.08 years) completed Medical Outcomes Study Short Form 36 before and after surgery. Scores from before and after surgery were compared. RESULTS: Statistically significant improvements (P < 0.01) were seen in eight of the nine health scores comparing scores from before and after surgery at follow-up evaluation. These included physical function, social function, role function resulting from physical limitations, role function resulting from emotional limitations, mental health, vitality, pain, and perceived health change. No significant change was seen in the patients' health perception after surgery. CONCLUSIONS: This study shows that the patients' self-reported health outcomes after lumbar laminectomy correlate with the excellent results previously seen using physician-driven outcome measures in an appropriately selected population with radiculopathy. The excellent results shown here did not deteriorate with age (> 40 years compared with < 40 years) or with complications after surgery.

Case-Control Studies↗
PubMed1996

Prenatal detection of fetal anomalies in pregnancies complicated by insulin-dependent diabetes mellitus.

OBJECTIVES: We evaluated the clinical utility of a comprehensive program of prenatal diagnostic testing for congenital anomalies in pregnancies complicated by insulin-dependent diabetes mellitus. STUDY DESIGN: Data were retrospectively analyzed from 289 diabetic women and their newborns from August 1987 to July 1993. Our protocol included initial hemoglobin A1 and maternal serum alpha-fetoprotein determinations and comprehensive fetal ultrasonography inclusive of a standard four-chamber view of the heart and detailed multiimage fetal echocardiography. RESULTS: Anomalies were identified in 29 of 289 (10%) fetuses and neonates: 12 cardiac only, 14 noncardiac, and 3 combined. In 21 of the 29 (72%) neonates the anomalies were detected prenatally. Twelve of 15 (80%) cardiac and 10 of 17 (59%) noncardiac lesions were identified prenatally. Cardiac lesions, especially of the cardiac septum and great vessels, accounted for 50% of all fetal defects. Malformations of the neuroaxis, skeleton, and genitourinary system were also detected. There were six neonatal deaths and four therapeutic pregnancy terminations associated with congenital anomalies. Although the hemoglobin A1 level was statistically significantly increased in 22 mothers of anomalous fetuses (p = 0.017), the actual difference between affected and nonaffected pregnancies was not clinically meaningful and much overlap occurred. Although 96% of women with a normal hemoglobin A1 level were delivered of normal infants, only 14% of those with an elevated value had a malformed fetus. Similarly, although 89% of gravid women with a normal maternal serum alpha-fetoprotein level were delivered of nonaffected fetuses, only 7.3% of patients with an elevated value had a malformed fetus. For the detection of cardiac defects, the sensitivity of the four-chamber view compared with detailed multiimage fetal echocardiography was 33% and 92%, respectively. CONCLUSIONS: This study demonstrates the utility of a comprehensive program to detect fetal anomalies in pregnancies complicated by diabetes mellitus.

Congenital Abnormalities↗
PubMed1995

Health outcome assessment before and after adult deformity surgery. A prospective study.

STUDY DESIGN: Fifty-five patients undergoing surgery for adult spinal deformity assessed their health status using a generic health outcome instrument before surgery and at an average of 2 years after surgery. OBJECTIVE: To assess whether adult spinal deformity surgery is a useful intervention when patients evaluate their own perception of health. The Medical Outcomes Survey Short Form-36, a validated generic health outcome assessment form, was used to measure patient's health status. SUMMARY OF BACKGROUND DATA: Surgery for adult scoliosis is technically demanding. No study that we are aware of uses a generic health outcome instrument in a prospective manner to compare patients' perception of their health after adult scoliosis surgery. The Medical Outcomes Survey Short Form-36 has been validated in multiple studies assessing other medical conditions and was found to be reliable, comprehensive, brief, and generic. METHODS: Sixty-eight adult patients undergoing surgery for adult spinal deformity were prospectively enrolled. Fifty-five patients were available to complete the Medical Outcomes Surgery Short Form-36 after surgery. The scores of the health profile were compared before and after surgery. The results of patients younger than 40 years were compared with those of patients older than 40 years. The results of patients younger than 40 years were compared with those of patients older than 40 years. The results of patients fused to more caudal end vertebral levels ((L4, L5)5) were compared with those who were fused to more cranial end vertebral levels. The results of patients without complications after surgery were compared with those of patients with complications after surgery. RESULTS: Average follow-up period was 22.5 months (minimum 12 months) in 82% of 68 patients. Statistically significant improvements were seen in postoperative scores for physical function, social function, bodily pain, and perceived health change. We found no significant differences in self-reported health function parameters related to age ( > 40 vs. < 40), end vertebral level of fusion, or presence of complications after surgery. CONCLUSIONS: Applying a generic health outcome instrument to adult spinal deformity surgery shows that adult scoliosis surgery significantly improves patient self-reported health assessment and function. Beneficial results do not appear to deteriorate with age or more caudal end vertebral levels of fusion. Future studies combining disease-specific outcomes analysis and generic health surveys to assess end results of adult spinal deformity surgery will be useful.

Adult↗
PubMed1994

Interleukin-1 beta regulates the inducible cyclooxygenase in amnion-derived WISH cells.

The purpose of this study was to determine the mechanism of enhanced prostaglandin synthesis in amnion-derived WISH cell cultures when stimulated by interleukin-1 beta (IL-1 beta). Confluent monolayer cultures of WISH cells were incubated with human recombinant IL-1 beta (0.001-10 ng/ml) for 0-24 hours, while control cells received medium alone. PGE2 production was measured by specific radioimmunoassay. IL-1 beta enhanced the production of PGE2 in a dose- and time-dependent manner with enhanced production detectable by 2 h following exposure. Immunoblot analysis using isoform-specific antibodies showed that the inducible cyclooxygenase enzyme, i.e., COX-2, was expressed by 2 h in IL-1 treated cells, while the constitutive COX-1 remained unaltered in its expression. Northern blot analysis demonstrated that COX-2 mRNA expression was not detected in untreated cells, but became evident after a 30-min exposure to IL-1 beta (10 ng/ml). COX-1 mRNA was detected under basal conditions and did not increase significantly following IL-1 beta treatment. The close parallel between the kinetics of COX-2 mRNA and protein expression and PGE2 accumulation in the medium, as well as the constitutive, unregulated nature of the COX-1 isoform, indicates that cytokine-driven PGE2 formation in WISH cells may be mediated by de novo expression of the novel COX-2 enzyme.

Amnion↗