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Biomedical subjects

T J Milson

Publications and source records attributed to T J Milson.

7 recordsLinked to original sources
PubMed1988

Immunologic response to Faenia rectivirgula (Micropolyspora faeni) in a dairy farm family.

In the present study, cellular and humoral responses to Faenia rectivirgula antigens were evaluated in seven subjects, members of a family who lived and worked on a dairy farm. Four subjects had clinical features of hypersensitivity pneumonitis after exposure to moldy hay. The other three subjects had no clinical disease in spite of similar exposure. Although serum precipitins were found in most subjects, a biotin-avidin-linked immunosorbent assay revealed high levels of F. rectivirgula-specific antibodies only in the symptomatic subjects. In addition, numerous precipitin arcs were present in the sera of the symptomatic but not the asymptomatic subjects by antigen-antibody crossed immunoelectrophoresis. No clear distinction between symptomatic and asymptomatic subjects could be made on the basis of lymphocyte phenotype studies, and antigen-induced lymphocyte transformation was not detected in any subjects. The results indicate that F. rectivirgula-specific antibody levels as detected by biotin-avidin-linked immunosorbent assay and by the presence of precipitin arcs in crossed immunoelectrophoresis may differentiate symptomatic and asymptomatic farmers.

Actinomycetales Infections↗
PubMed1986

Correlation of circulating natural killer cell count with prognosis in large cell lymphoma.

A monoclonal antibody recognizing a natural killer (NK) cell-associated antigen was used to sequentially quantify numbers of peripheral blood NK cells in a small group of patients with large cell lymphoma. Patients with active disease had low numbers (less than 100/mm3) of NK cells. Patients in complete remission after therapy had normal numbers of NK cells, but those patients who relapsed had a fall of NK cell number to less than 100/mm3 preceding or concurrent with clinical relapse. A role for NK cells in the surveillance and control of abnormal lymphoproliferation is suggested, as is the possible prognostic utility of sequential peripheral blood NK cell quantification in patients with large cell lymphoma.

Antibodies, Monoclonal↗
PubMed1986

Holding medium for cell surface phenotypic analysis.

Peripheral blood samples from 20 normal adults and 12 patients with diagnosed B cell lymphoproliferative disease were phenotyped with a panel of 20 monoclonal antibodies (Coulter) using a whole blood lysis technique at zero time and after storage in M199 containing insulin, transferrin and L-glutamine at 24, 48 or 72 h. The samples were analyzed using flow cytometry (EPICS V) to determine the percentage of positive events for each sample. A 2-way analysis of variance showed that there were no significant differences between the initial sample and those phenotyped after 24, 48 or 72 h. These data suggest that this medium can be utilized for the storage and transport of blood samples from patients and ensure satisfactory cell surface phenotypic analysis.

Adult↗
PubMed1986

Immunoregulatory abnormalities in myelodysplastic disorders.

Immunoregulation was assessed in a group of patients with myelodysplasia (MDS) by flow cytometric analysis of peripheral blood lymphocyte subsets and in vitro studies of mitogen-stimulated T-lymphocyte blastogenesis. Mitogenesis was significantly depressed in MDS patients compared to controls (p less than .001) and a similar defect was found in a small group of patients with untreated acute nonlymphocytic leukemia (ANLL) (p less than .005). The impaired mitogenic response ability of T-cells in these patients did not appear to be the result of alteration in lymphocyte subpopulation ratios. The observed defect might result from defective cooperation between T-lymphocytes and abnormal myeloid elements. Alternatively, the lymphocytes themselves could be derived from the abnormal clone and thus be functionally abnormal.

Antigens, Surface↗
PubMed1985

Flow cytochemical analysis of atopic reactions.

The technicon H-6000 has the unique ability among automated hematology systems to discriminate basophils from other hematopoietic cells on the basis of astra blue positivity of heparin-containing granules. We therefore examined the ability of this flow cytochemistry system to predict allergic diathesis in vitro by examining the number of basophils detected before and after incubation with a variety of allergens. One hundred subjects with documented atopic reactions to known allergens were examined and compared to 13 patients who had undergone successful desensitization and 12 normal subjects who had no history of atopy. One subject who had a previous anaphylactic response to penicillin but was skin-test negative to the major antigenic determinant demonstrated 55% degranulation after incubation with penicillin G. Finally, the basophil degranulation as assessed by the Technicon H-6000 was compared to a histamine release assay to determine the sensitivity of the automated system. The results suggest that flow cytochemistry may prove a valuable tool in predicting the success of desensitization therapy for common allergens and may have the potential to screen atopic reaction in the routine hematology laboratory.

Allergens↗
PubMed1982

The variable effect of cryopreservation on peripheral blood mononuclear populations.

Peripheral blood mononuclear cells (PBMC) were cryopreserved using 0.5 degree C/min or 1 degree C/min cooling rates. Following thawing they were evaluated by cell surface marker analysis and mitogen reactivity. T cells, B cells and monocytes were preserved when cooled at 0.5 degree C/min. B cells and monocytes were preserved when cooled at 1 degree C/min but T cell numbers decreased. Similarly FC gamma bearing T cells were preserved when cooled at 0.5 degree C/min but when cooled at 1 degree C/min they were decreased. Following thawing, incubation of PBMC with histamine induced or activated FC gamma bearing T cells. PBMC cooled at 0.5 degree C/min showed retention of nonspecific suppressor T cell activity in early cultures (96 hr) which could be induced and activated in late cultures (144 hr) whereas at 1 degree C/min nonspecific suppressor T cells were not preserved but could be induced and activated in late cultures (144 hr). Prostaglandin secreting monocytes retained functional activity independent of the cooling rates tested. Different cooling rates have variable effects on suppressor elements.

B-Lymphocytes↗