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T Jimi

Publications and source records attributed to T Jimi.

47 records · Page 3Linked to original sources
PubMed1989

Dystrophin immunostaining and freeze-fracture studies of muscles of patients with early stage amyotrophic lateral sclerosis and Duchenne muscular dystrophy.

We used polyclonal antibodies against dystrophin for the immunohistochemical localization of this protein in human skeletal muscle. Dystrophin was localized in the sarcolemma of the myofibers in 8 infantile and 11 adult normal control muscles and in 10 early stage patient muscles with amyotrophic lateral sclerosis (ALS). The protein was absent or markedly decreased in 8 early stage patients with Duchenne muscular dystrophy (DMD). Moreover the densities of sarcolemmal plasma membrane assemblies, orthogonal arrays and their pits were estimated by freeze-fracture electron microscopy studies in the same number of muscle samples in each disease and control case. The group median densities of orthogonal arrays and their pits in the ALS group and adult control group were 4.8 with a midrange of 1.1-13.5 (25-75%) and 7.5 with a midrange of 2.3-12.9, respectively (P greater than 0.1, Wilcoxon rank-sum test), whereas those of the DMD group and child control group were 0 with a midrange of 0-1.1 and 10.8 with a midrange of 5.4-16.7 respectively (P less than 0.01). The skeletal muscles of mdx mice and their controls were also investigated by the same techniques. In mdx mice, the absence or marked deficiency of dystrophin was also noted; however, the decrease of orthogonal arrays was not as severe as in DMD, which might relate to the milder clinical features in mdx mice as compared with those in DMD.

Adult↗
PubMed1989

[The effect of the cementing material on the strength of the all ceramic crown].

On the assumption that the all ceramic crown was cemented on an abutment tooth, the effect of the cementing materials on the strength of the ceramic crown was analyzed using two-dimensional finite element technique. The results obtained were as follows: 1. When the compressive load was applied to the ceramics, the principal stress in the cementing material was compressive in the region of Young's modulus of the cementing materials 200-700 kg/mm2, but was in tension beyond it. 2. When the tensile load was applied to the ceramics, the principal stress in the cementing material was in tension, and decreased as Young's modulus of the cementing materials decreased. 3. When the adhesive effect of the cementing material was investigated with examining the presence of the crack between the ceramics and the cementing material, the principal stress in the ceramics increased as the crack propagated. 4. The creation and propagation of the crack, the increase of the principal stress in the ceramics and the destruction of the ceramics were suggested. 5. When the analyzed results were compared with the mechanical properties of the cementing materials from the point of view of the breaking strength, it was considered that the adhesive resin cement was favorable as the cementing material of the ceramic crown. And the Young's modulus of the adhesive resin cement was about 400 kg/mm2.

Crowns↗
PubMed1989

[Effect of the 3/4 crown and porcelain-fused crown restorations on the tooth displacement and the loading force of the supporting tissues].

A mechanical simulation model of tooth and alveolar bone in the application of 3/4 crown and porcelain-fused crown restorations was made and analyzed using three-dimensional finite element technique. The results obtained were as follows. 1. When the physiological tooth mobility was less than 150 microns, the teeth were sufficiently fixed using the splint and the bridge connected with more than two teeth, in the case of 20 percent absorption of the alveolar bone, and more than three teeth in the case of 40 percent absorption. In the case of 60 percent absorption, the teeth were fixed with the splint of six teeth connections, and were fixed with the bridge of five teeth connections. 2. The periodontium supporting force decreased markedly according to the increase of the number of the abutment teeth. 3. The teeth accompanied with the absorption of the alveolar bone were connected, in order to inhibit the tooth mobility and the periodontium supporting force. In this application, it had the inhibitory action when the replacing ratio of metal and ceramics to a crown of the tooth was less than 35 percent. 4. According to the finite element analysis, the design bases of fixed restorations applied in the teeth accompanied with the absorption of the alveolar bone were preferred.

Alveolar Bone Loss↗
PubMed1989

[Two cases of Creutzfeldt-Jakob disease with high neuron-specific enolase level in cerebrospinal fluid].

Because neuron-specific enolase (NSE), one of the distal branch enzyme of Embden-Myerhof glycolytic pathway is abundant in the neuronal cytoplasm and axons, the measurement of its level in cerebrospinal fluid (CSF) would be useful in diagnosis and consideration of pathophysiology in various neurological diseases. The Creutzfeldt-Jakob disease (CJD) in which neuronal destruction is usually prominent pathologically, has so far been thought to reveal no abnormalities in CSF. In the two cases of CJD, we conducted the time sequential measurement of NSE level in CSF and compared it with serum NSE and CSF lactate levels. We found that the CSF NSE level was high in the early stage of this disease, at which time the brain CT showed no or minimal abnormalities, followed by gradual increase up to the maximum level when myoclonus and periodic synchronous discharge appeared in electroencephalogram. Then, the CSF NSE level decreased in parallel with the progression of brain atrophy in CT scan and finally on the late stage, the CSF NSE level fell within normal range. Serum NSE and CSF lactate levels were mildly elevated in all stages of this disease, but were not parallel to the disease activity. This implies that the CSF NSE level can serve as a marker for the presence of active process in neuronal destruction. Again the CSF NSE level was elevated in both cases even from the very early stage when the typical clinical manifestations of this disease were absent. Therefore, the results of this study provided us with an important indicator for the early stage diagnosis of CJD.

Aged↗
PubMed1987

Freeze-fracture analysis of cholesterol in muscle plasma membrane of Fukuyama-type congenital muscular dystrophy.

We used digitonin in freeze-fracture analysis of the muscle plasma membrane cholesterol content in six patients with Fukuyama-type congenital muscular dystrophy and six control children. A significantly greater proportion of surface area was taken up by digitonin-cholesterol complexes in the patients (51.2% +/- 4.7%) than in controls (31.9% +/- 2.9%) (P less than 0.01). Since membrane cholesterol has a dynamic regulatory function in affecting the activity of membrane-bound proteins, the increased cholesterol content in the patients suggests a functional abnormality of the muscle plasma membrane in this disease.

Cell Membrane↗
PubMed1986

Small size of orthogonal array in muscle plasma membrane of Fukuyama type congenital muscular dystrophy.

Freeze fracture analysis was carried out on the density of orthogonal array subunit particles in the plasma membrane of skeletal muscle of six patients with Fukuyama type congenital muscular dystrophy and seven control cases. The group mean density of orthogonal array subunit particles per one orthogonal array was significantly lower in the plasma membrane of Fukuyama patients. The results suggested the possible impairment of orthogonal array function in the plasma membrane of muscle fibers in congenital muscular dystrophy of the Fukuyama type.

Cell Membrane↗
PubMedDate not supplied

[Reversible posterior leukoencephalopathy in a patient receiving cyclosporin therapy].

We reported a case of reversible posterior leukoencephalopathy syndrome (RPLS) that occurred during cyclosporin A (CyA) therapy for fulminant hepatitis. A 22-year-old man was given an intravenous drip of interferon-beta, metylprednisolone sodium succinate and CyA, and also received plasma exchange and hemodiafiltration. On the 7th day of the intravenous CyA therapy, in which its dose had been increased from 60 mg/day to 84 mg/day, he became somnolent and had headache, double vision, hallucination and then a generalized tonic-clonic seizure. The blood CyA concentration increased to a level as high as 455 ng/ml. Brain computed tomography (CT) scan without contrast medium revealed symmetric low-density areas in the bilateral occipital white matter and partly in the cortex. T2-weighted magnetic resonance imaging (MRI) showed an increased signal intensity, and single-photon emission CT using 99 mTc showed a hypoperfusion of cerebral blood flow in those areas. After CyA administration was changed to 100 mg/day orally to decrease its uptake in the blood, his consciousness and vision recovered within 4 weeks. Then abnormalities in MRI findings completely disappeared. On the basis of the clinical course and time-sequential change of serum CyA level in this patient, he was diagnosed as having RPLS caused by CyA therapy. Recently, the number of cases of RPLS has increased in the Western countries. However, there are few reports of RPLS after CyA therapy in Japan. From this case, we emphasize that careful following up the patient's neurological findings during CyA therapy is very important and that a cranial MRI is an essential tool for the diagnosis of RPLS.

Adult↗