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Biomedical subjects

T K Basu

Publications and source records attributed to T K Basu.

At least 91 records · Page 5Linked to original sources
PubMed1979

The effect of megadose ascorbate on some haemopoietic factors in the young guinea pig.

The effect of oral administration of megadoses of ascorbate was studied on some haemopoietic factors in young apparently normal guinea pigs. Supplementation of 150 mg of the vitamin for 7 days resulted in significantly increased haematocrit value and haemoglobin concentration. Total and unsaturated iron binding capacity were also increased in association with elevated plasma iron concentration. The difference of these results from the control animals, however, was not statistically significant. The improved haematological status in guinea pigs by ascorbate treatment could be attributed to haemoconcentration rather than to an effect of iron metabolism.

Animals↗
PubMed1978

The effect of ascorbic acid (vitamin C) on two tumor cell lines in culture.

The effect of vitamin C on cell proliferation and DNA synthesis was investigated using two tumor cell lines Hep2 and KB. The results show that there was an increase in the ratio of dead to live cells and a decrease in the rate of DNA synthesis. The results also agree with other work carried out on animal experimental models which claimed that vitamin C may be involved in the arrest of neoplastic cell proliferation.

Ascorbic Acid↗
PubMed1978

Ascorbic acid therapy for the relief of bone pain in Paget's disease.

Sixteen patients with painful Paget's disease of the bone were treated with high doses of ascorbic acid. Of these patients, 8 experienced lessening of pain within a period of 5 to 7 days after commencing the vitamin therapy. In 3 of these patients pain was completely abolished. Subsequent treatment with calcitonin caused improvement in most cases. There was little change in plasma alkaline phosphatase levels but the excretion of hydroxyproline was elevated following administration of the vitamin. The highest excretions were found in those patients who experienced complete relief of pain. In patients treated with calcitonin alone, the excretion of hydroxyproline was reduced and urinary levels of ascorbic acid dropped in parallel. It seems clear that ascorbic acid and calcitonin have different effects upon bone metabolism.

Aged↗
PubMed1976

Effect of protein-energy nutrition on the activity of hepatic microsomal drug-metabolizing enzymes in growing rats.

Male Wistar weanling rats were divided into three groups and fed one of the following diets for 7, 14 and 28 days: a control diet (21% protein) fed ad libitum, a low protein diet fed ad libitum (the control diet diluted to contain 7% protein by the addition of starch) or an energy and protein restricted diet (control diet fed to provide protein intake equal to that consumed by the low protein group). The activity of glucoronyl transferase was depressed by energy restriction and cytochrome P-450 was depressed by the low protein-ad libitum energy diet compared to controls. In the animals fed the low protein diet with ad libitum energy for 14 or 28 days, the rise in the activity of biphenyl 4-hydroxylase per g liver was sufficient to compensate for the lower liver weight so that the absolute amount of enzyme activity per liver was the same as in the controls. Due to adaptive response of the enzyme to the low protein diet, the activity of biphenyl 4-hydroxylase per g liver was higher than in the controls in animals fed a protein-free diet. It is concluded that the adaptive response of biphenyl 4-hydroxylase was possibly mediated by corticosteroids and was not due to a change in the conformation of the enzyme.

Animals↗
PubMed1976

Significance of vitamins in cancer.

The relationship of vitamins to cancer is very complex. Three types of interactions are possible: the effect of vitamins on tumor growth, the effect of tumors on vitamin metabolism, and the effect of vitamins on chemical carcinogens and anti-tumor chemotherapeutic agents. The significance of vitamins with particular references to vitamins A,B-complex and C, in cancer has been reviewed.

Antineoplastic Agents↗
PubMed1976

The thiamin status of early cancer patients with particular reference to those with breast and bronchial carcinomas.

The thiamin status of early bronchial and breast carcinomas has been assessed using "thiamin pyrophosphate" (TPP) stimulating effect of transketolase enzyme activity. The TPP stimulating effect was found to be greater in a high proportion of the cancer patients than in normal subjects, suggesting that these patients may be at risk of thiamin deficiency. The urinary excretory level of thiamin was high in the cancer patients; it is, therefore, unlikely that the biochemical deficiency of the vitamin was due to lack of the vitamin intake. It is, however, possible that the cancer patients were unable to convert thiamin to thiamin pyrophosphate, the active form of the vitamin.

Adult↗
PubMed1976

Blood values of some vitamins in long-stay psycho-geriatric patients.

A group of 18 long-stay hospital psycho-geriatric patients (aged 64-101 years) and a group of 10 healthy elderly subjects (aged 53-67 years) who were living in their own residence were studied. The group of geriatric patients were suffering from some degree of pressure sores, dementia, irritability and anorexia. Biochemical tests on vitamin adequacy with particular references to ascorbic acid, thiamine and retinal were performed in the subjects. The thiamine status was measured by determining the thiamine pyrophosphate stimulating effect of transketolase enzyme activity in whole blood. The ascorbic acid and retinal nutritures were determined by measuring their levels in plasma. From the biochemical data of the vitamins it would appear that a significant proportion of the long-stay hospital psycho-geriatric patients may be at risk of thiamine, ascorbic acid and retinol deficiencies.

Age Factors↗
PubMed1975

Effect of protein/energy nutrition on rat plasma corticosteroids and liver microsomal hydroxylase activity.

Three groups of 24-day-old male Wistar rats were fed (a) a control diet containing 210 g/kg protein ad libitum (control) (b) a low protein diet consisting of the stock diet diluted with starch to contain 70 g/kg protein ad libitum (protein deficient) or (c) an amount of stock diet containing the same amount of protein as eaten by (b) but without starch (energy deficient). The low protein diet did not affect the ability of the animals to metabolize biphenyl and excrete it as 4-hydroxybiphenyl, or reduce the total activity of 4-hydroxylase per liver, whereas this was reduced by restricted amounts of the high protein diet. The low protein diet resulted in raised levels of corticosteroids in the plasma. It is concluded that the raised specific activity, and unchanged total amount of biphenyl 4-hydroxylase per liver of the low protein animals is an adaptation to the diet mediated by corticosteroids as exogenous corticosteroids were also found to increase the activity of this enzyme in normal animals.

11-Hydroxycorticosteroids↗
PubMed1975

Effect of dietary substitution of sucrose and its constituent monosaccharides on the activity of aromatic hydroxylase and the level of cytochrome P-450 in hepatic microsomes of growing rats.

The effect of dietary substitution of starch by sucrose, glucose, fructose or an equimolar mixture of glucose and fructose on the activity of biphenyl hydroxylase and the level of the major terminal mixed function oxygenase, cytochrome P-450, has been studied in hepatic microsomal preparations from growing rats. The substitution of sucrose for starch depressed the contration and total activity per liver of biphenyl 4-hydroxylase and the concentration, but not the total amount, of cytochrome P-450 in weanling rats. The absolute amount of these enzymes in whole liver was not, however, similarly depressed by the constituents of sucrose, namely glucose and fructose, either when given alone or in equimolar mixture. All these sugars, however, depressed the total activity of biphenyl 2-hydroxylase. The activity of biphenyl 4-hydroxylase in the liver of weanling rats, but not of adults, was reduced when the level of sucrose in the diet was reduced from 60 to 10%.

Animals↗
PubMed1975

Effects of methotrexate and phenobarbital on the hepatic microsomal drug-metabolizing enzymes in normal rat.

The effect of methotrexate on the NADPH-dependent hepatic microsomal drug-metabolizing enzymes of the normal rat has been investigated. Pre-treatment of the animals with methotrexate for 3 successive days resulted in significant increases in the activities of biphenyl hydroxylase and ethylmorphine N-demethylase. The activity of these enzymes obtained from liver microsome preparations was also increased by methotrexate in vitro. The body weight, liver weight and the amount of both protein and DNA in each liver were significantly depressed by methotrexate treatment. All values, except the body weight, were increased to the control levels by additional treatment of the animals with phenobarbital. A likely explanation for the increase of enzyme activity by methotrexate has been discussed.

Animals↗
PubMed1975

Plasma and body lipids in patients with carcinoma of the breast.

Patients with breast carcinoma had elevated levels of plasma total lipids, phospholipids and cholesterol which were associated with increased amount of subcutaneous body fat as compared with the patients bearing non-breast carcinomas. The raised plasma concentrations of these parameters in the patients bearing breast carcinoma may be due to increased rate of lipid absorption as the fat-splitting enzymes, lipases, were also found to be increased in the patients.

Adipose Tissue↗