PubMed Health⌕ Search

Biomedical subjects

T K Maitra

Publications and source records attributed to T K Maitra.

At least 19 recordsLinked to original sources
PubMed1992

Virulence of nonpathogenic zymodemes of Entamoeba histolytica isolates from asymptomatic subjects in Calcutta, India.

1. The zymodeme and virulence of 31 Entamoeba histolytica isolates obtained from asymptomatic human subjects in Calcutta, India are described. 2. Virulence was measured by the extent of lesion diameter (mm) induced by each isolate in the liver of golden hamsters and resistance of isolates to non-immune hamster sera in vitro. 3. Two nonpathogenic zymodemes, III (N = 17) and IV (N = 14), were detected among 31 isolates by isoenzyme electrophoresis. Most of the zymodeme III isolates were moderately to highly virulent while a quarter of the zymodeme IV were invasive although with low virulence. 4. The virulence of the isolates was found to have a significant positive correlation (r = 0.96, P < 0.001) with their greater resistance to complement-mediated lysis. 5. The data suggest that the virulence of E. histolytica is probably not related to its zymodeme.

Animals↗
PubMed1991

Enhancement of virulence of Entamoeba histolytica by in vitro liver treatment.

Enhancement of the virulence of five strains of Entamoeba histolytica (three xenically maintained and two axenically maintained) was studied after in vitro incubation with normal hamster liver. Increased virulence was shown by the ability of a small number of liver-treated trophozoites to produce liver lesions in hamsters. Enhancement of virulence was positively correlated with increased resistance to normal hamster serum complement in vitro.

Animals↗
PubMed1991

Study of Entamoeba histolytica isolates from Calcutta with special reference to their zymodeme, animal pathogenicity and erythrophagocytosis.

Entamoeba histolytica isolates, obtained mainly from asymptomatic human cyst passers, were tested for their in vitro erythrophagocytic activity and lesion-forming ability in hamster liver. Simultaneous zymodeme typing of the isolates showed that, along with the pathogenic zymodemes, a number of isolates having nonpathogenic zymodemes also produced liver lesions in hamsters and engulfed higher numbers of RBCs. The observed positive correlation between the rate of erythrophagocytosis of an E. histolytica trophozoite and its degree of lesion-producing capacity in hamster liver suggest that the erythrophagocytosis assay in conjunction with the hamster liver inoculation test, can be useful in the identification of virulent E. histolytica isolates.

Animals↗
PubMed1990

The prediction of serum IgG levels by scanning densitometry.

A novel approach has been described. In this method the serum IgG level can be predicted with a fair degree of accuracy by scanning densitometry and total serum protein values. This approach might be useful as a screening procedure for hypogammaglobulinemia.

Agammaglobulinemia↗
PubMed1990

Experimental hepatic amoebiasis in inbred mice.

A model of hepatic amoebiasis in two inbred strains of mouse (Swiss and BALB/c, has been developed by introducing Entamoeba histolytica-infected hamster liver tissue in between the adjacent liver lobes of mouse. It is expected that this model might be useful in studying various parameters of host-parasitic interactions and experimental chemotherapy of amoebiasis with relative ease.

Animals↗
PubMed1989

A specific solid-phase assay for the detection of immune complexes containing Entamoeba histolytica antigens.

An assay specific for immune complexes containing Entamoeba histolytica antigens has been developed. Rabbit antibody specific for human gamma globulin is attached to a solid phase (nitrocellulose). During the first incubation step polyethylene glycol-precipitated immune complexes are bound to the rabbit antibody attached to the solid phase. In the second incubation step a radiolabelled rabbit antibody specific for Entamoeba histolytica combines with E. histolytica antigens in the complexes. Quantitation of the radiolabel provides a direct measurement of the level of specific immune complexes. The principle of the method has been verified using artificial soluble immune complexes prepared by mixing a pool of serum containing a high titre of anti-E. histolytica antibodies (greater than 1:2048 by hemagglutination assay) with the E. histolytica antigen. This antigen was prepared from axenically cultured NIH:200 strains of Entamoeba histolytica. Using sera from patients with clinical amoebic disease this method detected immune complexes containing E. histolytica in a high proportion of cases.

Animals↗
PubMed1987

Slow-release potassium and perforation of Meckel's diverticulum.

A case of peritonitis following perforation of gastric-type mucosa in a Meckel's diverticulum is described. At laparotomy a wax core of a combined diuretic-slow-release potassium preparation was found extruding through the perforation and a further pellet was found free in the peritoneal cavity. Intestinal perforation due to potassium-containing drugs is recognized and led to the development of enteric-coated and slow-release delivery systems. The association of perforation of Meckel's diverticulum with a slow-release potassium perforation has not previously been described.

Aged↗
PubMed1985

A simple ELISA for antigiardial antibody using the membrane fraction of the parasite as antigen.

Most existing methods for detecting antigiardia lamblia antibody require the use of cultured Giardia lamblia trophozoites as antigen in immunofluorescent and enzyme-linked immunosorbent assays. However, the maintenance of trophozoite culture systems limits the large-scale use of these antibody detection systems. An antigen extracted from Giardia lamblia by the detergent Triton X-100, when used in an ELISA system, produces specific, objective, quantitative and reproducible results. It is likely that such a test system could be packaged in kit form for large-scale diagnostic and epidemiological application in all parts of the world.

Animals↗