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T Kagabu

Publications and source records attributed to T Kagabu.

33 records · Page 2Linked to original sources
PubMed1987

[Scanning electron microscopic studies on the cell surface and cell synchronized culture of human endometrial adenocarcinoma (HEC-1) cells].

The cell cycle of cultured human endometrial adenocarcinoma cells were observed with a SEM using the synchronous culture method and the following results were obtained. In the M phase, the cells changed morphologically to become a minimum size and rounded in shape. Filopodia became thin and straight and reached their maximum length. Moreover, blebs reaches their greatest number. In the G0+1 phase, the cells developed from a round shape to an oval shape, and finally became polymorphic. The ends of the filopodia degenerated and became shortened. The number of blebs was decreased only in this stage. During the S phase, the cells became slender and reached maximum size. Short filopodia reappeared. No blebs but the largest microvilli were found. When we compared the late G1 with the early G2 phase, we found polymorphism of the cells and coexistence of microvilli and blebs common to both phases. However, ruffles were observed only in the former phase, while in the latter phase renewal of short filopodia together with thick and bent filopodia was evident. When we compared the late G2 with the early G1 phase, we found that the cells were small in size and round shape, and that the existence of blebs was common to both phases. However, the tips of the filopodia were destroyed in the latter phase, while in the former phase thick and bent filopodia were observed.

Adenocarcinoma↗
PubMed1985

[The relationship between changes of the steroid receptor and synchronization in human adenocarcinoma cells in vitro of the endometrium].

We investigated the G1 and S phases of synchronization using sodium n-butyrate, MTX and excess thymidine. The flow cytometry system was employed for cell cycle analysis while the receptor assay was adopted dextran coated charcoal (DCC) and the wash method. The results were as follows: S phase synchronization by MTX was 138% (control 100%) and by excess thymidine in the block and release method 210%. G1 phase synchronization by sodium n-butyrate was 140%. The progesterone receptor level, by E2 priming increased to 1.45 fmol/ug DNA being more than five times that of the control PR. The estrogen receptor level increased to 18.29 fmol/ug DNA in the G1 phase synchronization, seven times that of the control ER. From this study, the functional increase of the steroid receptor was most significant in the G1 phase.

Adenocarcinoma↗
PubMed1985

[Flow cytometric study on the action of progestogen in patients with adenocarcinoma and precancerous lesions of the endometrium].

Synthetic progestogens have been used in 21 patients with endometrial adenocarcinoma and 6 patients with precancerous lesions. During a program of management with medroxyprogesterone acetate (MPA) in an attempt to identify the mechanism by which a remission rate in 30-40% occurred, adenocarcinoma cells were analysed. Histopathologic reactions of endometrial adenocarcinoma treated with MPA 400mg/day through 7 days have been found similar those of normal secretory endometrium such as subnuclear vacuolation, loss of mitotic figure, diminished nuclear size and stromal decidual reaction and regressive changes are highly dose dependent. In detailed analysis of the cell cycle, computerized flow cytometry was employed to quantitate total DNA content following propidium iodide fluorescent staining after RNase treatment. Possible modes of action of MPA on endometrial adenocarcinoma are an increase in the G0+1 fraction corresponding to ploidy reduction. Adenomatous hyperplastic lesions were able to be reversed completely in all patients compared with adenocarcinoma in sixty-six percent.

Adenocarcinoma↗
PubMed1983

[Changes in immunoglobulin-positive cell populations in the vaginal smear during radiotherapy of cervical cancer and their relation to 5-year survival rate].

To study the immunological response of irradiated cervical cancer, vaginal smears were taken from patients with this neoplasm during radiotherapy and stained with fluorescent anti-Igs sera by a direct method. Then, the relationship between the immune response during radiotherapy and a five year cure were discussed. The results obtained were as follows: 1. The mean percentage of the surface IgM-positive cells was 1.9% in normal vaginal smears and 5.8% in cervical cancer. 2. On the changes in the surface IgM-positive cells during radiotherapy, the patients were classified into three groups. Group A: The number of surface IgM-positive cells increased temporarily during radiotherapy. Group B: The number of surface IgM-positive cells decreased gradually during radiotherapy. Group C: The changes in the surface IgM-positive cells were irregular and/or showed little deviation. The five year survival rate for Group A was 81.8% and for Group B was 50.0%. There was significant difference between Group A and Group B in survival rates, and Group A was considered to be an immunoreactive group.

Female↗
PubMed1983

[Optimal frequency of cervical cancer cytology for mass-screening].

It is recommended that, as a general rule, all asymptomatic women in Japan undergo cervical cytology for cancer detection once a year. However, a new guideline issued by the American Cancer Society states that screening frequency may be reduced to once every three years except in certain cases. In our cancer screening program, a total of 434,773 women who had cervical cytology were evaluated according to cytological abnormalities and the following results were obtained: 1) The prevalence of cancer in women examined for the first time (0.175-0.42%) was lower after repeated examinations (0.02-0.03%) and gradually reached the incidence rate. 2) Less that 0.016% developed cervical cancer after continuing to have a Class I grading for two years and we failed to detect invasive cancer in these cases. It is therefore considered sufficient for them to have a Pap test once every two years. 3) When we employed more limited criteria than those of Papanicolaou's classification, we derived a lower incidence rate for those who had maintained a Class I grading for two years continuously. 4) There was a remarkably high prevalence rate in old women, and because there are as yet no established criteria for atrophic smear cytology, we could not fix on the most desirable frequency of cervical cancer cytology for them.

Adult↗
PubMed1981

[Histopathologic and cytologic studies on uterine sarcomas (author's transl)].

Non-epithelial tumors originating in the uterus are a relatively rare affliction, accounting for less than 3% of all malignant uterine neoplasms. For this reason, there are at present few cytological reports on these sarcoma, so that histological determination on the basis of cytological diagnosis becomes quite difficult. On this occasion, We are privileged to report on cytological examinations we have made over the past decade on six cases involving uterine sarcomas in the course of our practise at the Iwate Medical University School of Medicine. The histological diagnosis of these tumor were Leiomyosarcoma (1 case) Mixed mesodermal tumor (1 case). Rhabdomyosarcoma (1 case). Carcinosarcoma (2 cases), and Mixed cell sarcoma (1 case). The cytologic findings of these tumor cells in vaginal smear and aspiration smear of uterine cavity are as follows: 1) In all cases, We observed the appearance of tumor cells of undifferentiated non-epithelial origin, showing an almost identical configuration. 2) Also, in these various sarcomas, We confirmed distinctive tumor cells from which histologic type could be inferred. This suggested the possibility of histologic type diagnosis on the basis of cytological diagnosis.

Adolescent↗