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T Kasajima

Publications and source records attributed to T Kasajima.

At least 73 records · Page 4Linked to original sources
PubMed1986

Immunocytochemical study on the immune complex and germinal center of synovial tissue of rheumatoid arthritis with special regard to complement and follicular dendritic cells.

To analyze the immunological role of lymphoid germinal centers and follicular dendritic cells (FDC) in synovial tissue of rheumatoid arthritis (RA), we tried to detect the immune complex in germinal centers by light and electron microscopic immunocytochemistry with special emphasis on immunoglobulin, complement components, RA factor, and DRC-1 antigen. Immunoglobulins mainly distributed intercellularly in the germinal center in a lacy network pattern, and show partial intracytoplasmic localization in some germinal center lymphoid cells. Early complement components of classical pathway (C1q, C4, C3c, and C3d) and RA factor distributed lacily similarily to immunoglobulin, but intracytoplasmic positivity is never observed. These coexistent positive sites are identical to DRC-1 positive sites which are the surface of extended processes of FDC membrane. A similar finding is observed in primary follicles or lymphoid aggregates less often than germinal centers. These results indicate that some germinal centers trap the immune complex, including RA factor at least closely related with FDCs, and also RA factor is one of the triggers of antigen as well as developing germinal centers.

Antibodies, Monoclonal↗
PubMed1986

Function of the follicular dendritic cell in the germinal center of lymphoid follicles.

The authors made an immunocytochemical examination of the germinal centers (GCs) of (1) lymph follicles in physiological lymph nodes and (2) extra-nodal tissues of divergent diseases including thyroid disorders, rheumatoid arthritis, Warthin's tumor and Kimura's disease (Eosinophilic lymphfolliculoid granuloma). In these germinal centers, the presence of immunoglobulins (IgG and IgM), early acting complement components (C1q, C4, C3c, C3d), receptors for C3b and C3d and dendritic reticulum cell-1 was demonstrated in lace-like network patterns which were proven electron-microscopically to coincide with the surface of follicular dendritic cells. IgE was distributed in a lace-like pattern in the GCs of proliferating follicles of Kimura's disease, in which the lysis of follicles was frequently observed. This lysis appeared to be related to the presence of complement components. In the germinal centers of extra-nodal tissues, including the thyroid tissues accompanying the lymph follicles, rheumatoid arthritis synovial tissues as well as Warthin's tumors, thyroglobulin, rheumatoid factor and salivary amylase were detected as specific antigens, occurring in lace-like patterns. It is possible that follicular dendritic cells may play a role in the genesis of GCs and be responsible for the immune response through C3 receptors.

Adenolymphoma↗
PubMed1985

An immunocytochemical study on secretory mechanism of IgA in human pancreas.

To analyze the secretory mechanism of immunoglobulins into pancreatic secretion, pancreatic juice IgA was measured by single radial immunodiffusion and characterized immunochemically, and immunoglobulins, J chain and secretory component (SC) were localized immunocytochemically in pancreatic tissues by the peroxidase-labeled antibody method. The concentration of IgA was 0.0225 mg/ml with a range of 0.010 to 0.049. IgA associated with SC was demonstrated immunochemically in three cases. IgA, SC and J chain were demonstrated immunocytochemically in the pancreatic duct epithelium with the features characteristic of endocytic, SC-mediated transfer of IgA. The results of the present study suggested that transfer of IgA linked by J chain and SC across ductal epithelial cells, but not acinar cells occurred in human pancreas.

Biological Transport↗
PubMed1983

[Study of lysosomal enzymes in human synovial membrane and fluid from rheumatoid and non-rheumatoid patients].

In rheumatoid arthritis, it is well known that lysosomal enzymes such as lysozyme and acid phosphatase have a function of destroying bone and synovial tissue of joints. In order to analyze the localization and the difference of distribution of lysozyme and acid phosphatase on the synovial tissue of rheumatoid arthritis (RA) and non-RA joints, immunohistochemical and histochemical methods were employed. Lysozyme was detected with formalin-fixed, paraffin-embedded materials in 82 cases of synovial tissue (RA 50 cases, non-RA 32 cases) using the unlabelled peroxidase anti-peroxidase (PAP) method following Taylor, et al. Acid phosphatase was detected with the naphthol AS method using frozen sections. In addition, in some cases of RA, alpha 1-antitrypsin and alpha 1-antichymotrypsin were also examined in synovium by the PAP method. For quantitative analysis of lysozyme in synovial fluid, lyso-plate were used on 98 cases (RA 58 cases, non-RA 40 cases). Further, acid phosphatase was quantitated with phenyl phosphoric acid. The results show, histologically, that lysozyme was more predominantly and more specifically located in the synovial cells, especially in the synovial lining cells of RA joints than non-RA joints. Lysozyme was distributed in the cytoplasm of synovial cells in a fine granular or small globoid pattern. On the other hand, no lysozyme was detected on the infiltrated lymphocytes and plasma cells. Infiltration of leukocytes was relatively slight. Acid phosphatase was intensively located in the same portion of RA synovium as that of lysozyme. Electron microscopically, synovial surface cells showed an increase in number, and they contained dominant, well-developed, rough endoplasmic reticulum and electron dense bodies. Fibrillar matrix were present in the cytoplasm and in the extracellular space in an amorphous pattern. Enzyme activity of lysozyme in 58 RA synovial fluid was 113 +/- 101 (mean +/- standard deviation) micrograms/ml and that in 40 non-RA (11 osteoarthritis, 20 autopsy cases, and others) was 35 +/- 31 micrograms/ml. Acid phosphatase activity of 47 RA was 11.97 +/- 10.45 I.U. (International Unit) and that of 38 non-RA was 5.16 +/- 3.77 I.U. A significant difference of lysosomal enzyme activity was thus found in the synovial fluid between RA and non-RA. Clinical laboratory data, namely, ESR (erythrocyte sedimentation rate) and CRP (C-reactive protein) as an activity of rheumatic disease were evaluated. Correlation rate between ESR and lysozyme in RA synovial fluid was 0.279 (the value of P less than 0.05) and between ESR and acid phosphatase was 0.259 (P less than 0.05). Thus no significant correlation was found among them.(ABSTRACT TRUNCATED AT 400 WORDS)

Acid Phosphatase↗
PubMed1983

[Effect of cepharanthin on peripheral leukocytopenia caused by antineoplastic agents].

One of the problems in administration of anticancer drugs is a decrease of peripheral blood leucocytes which causes reduction of defense mechanism against various infections. In this study, for the purpose of protecting leucopenia after administration of anticancer drugs, an effect of cepharanthine which is a kind of alkaroid materials was examined in normal and tumor-bearing mice. Peripheral leucocytes of both normal and tumor-bearing mice receiving anticancer drugs gradually decreased during first two weeks, and in third and forth week they decreased rapidly. On the other hand, leucopenia was improved by simultaneous administration of anticancer drugs and cepharanthin; however, a complete repairment was hardly expected when anticancer drugs were continuously administered. A significant recovery of leucocytes was observed in mice receiving cepharanthin compared to those receiving only anticancer drugs. In any process of decreasing and recovering from peripheral leucocytes, proportions of differential counts of leucocytes were approximately identical. On the colony forming experiment for granulocytic stem cell (CFU-C) using bone marrow cells of normal, anticancer drugs-treated, and anticancer drugs plus cepharanthin-treated mice, it has been suggested that cepharanthin might have some influencing activity on hematopoietic cells or cell-mediated activity on hematopoiesis only in vivo.

Alkaloids↗
PubMed1981

An electron microscopic study on the reticuloendothelial cells in the lymph nodes.

In an attempt to clarify the cytological characteristics of the RES cells in the lymph nodes and their embryological correlations, lymph nodes and lymph node anlages of germ-free rats, nude mice, and human fetuses were light and electron microscopically examined. On the basis of differences of intracellular organelles, their behaviors for reticulum fibers and of endogeneous peroxidatic activity, histiocytes should be reasonably distinguished from the cells conventionally called reticulum cells. Reticulum cells and histiocytes respectively are destined to differentiate in different directions from the early stage of development of the lymph node anlage. Sinus endothelial cells are ontogenetically originated and differentiated from the endothelial lining cells of lymphatic vessels. Primitive reticular cells are differentiated into mature reticulum cells in the lymph nodes, they transform into the lympho-reticular cells, further into lymphoblasts, and finally develop into medium-sized lymphocytes.

Animals↗
PubMed1981

Malignant fibrous histiocytoma of bone associated with focal hemangiopericytomatous pattern.

A case of malignant fibrous histiocytoma of the tibia of a 49-year-old Japanese farmer was presented. The patient was diagnosed as malignant hemangiopericytoma by biopsy, whereas the amputated material showed a marked pleomorphism representing features of malignant fibrous histiocytoma. Ultrastructurally, undifferentiated mesenchymal cells, intermediate cells between the undifferentiated mesenchymal cells and fibroblastic cells, fibroblastic cells and histiocytoid cells including bizarre giant cells were confirmed in a broad modulating spectrum. The hemangiopericytomatous lesion was predominated by intermediate cells, the ultrastructure of which bore a close resemblance to that of pericytes, and was regarded to be resulted from a differentiation of undifferentiated mesenchymal cells towards the pericyte.

Bone Neoplasms↗
PubMed1980

Non-hodgkin malignant lymphoma with special reference to desmosome-like junctions.

Seventy-nine cases of non-Hodgkin malignant lymphoma were light and electron microscopically evaluated with special reference to intercellular correlationship comparing with non-neoplastic lymph nodal cells and cultured B cells. Reticulum cells and histiocytes are regarded as an independent cell unit. Reticulum cell sarcoma shows a fibrosarcomatous proliferation with abundant reticulin fibers, while histiocytic sarcoma is confined to a pleomorphic neoplasm with valid phagocytization. Lymphoid cells are divided into large, intermediate and small lymphocytes. Under inflammation and cultivation they, especially intermediate and large, preferentially proliferated clustering in a reticular fashion, which are designated as reticular lymphocytes, in contrast with nonreticular lymphocytes individually separated. The desmosome-like junctions are found among reticular lymphocytes not only in but also outside germinal centers as well as in vessels and among cultured B cells. Lymphocytic sarcomas, either nodular or diffuse, are classified into reticular and nonreticular types in association with their cellular sizes. The desmosome-like junctions are predisposedly disclosed in reticular lymphoma irrespective of proliferating patterns, nodular or diffuse, and of membrane character, B or T. And the junctions are not regarded as a hallmark for follicular or nodular lymphomas of germinal center cell origin. ACTA PATHOL. JPN. 30: 195 approximately 217, 1980.

B-Lymphocytes↗
PubMed1980

Watery diarrhea, hypokalemia and achlorhydria syndrome. Morphological and immunohistological study.

A case of WDHA syndrome accompanied by a pancreatic tumor in a 44-year-old Japanese male is presented, the 6th case in Japan. Clinically, the patient suffered from unremitting watery diarrhea, hypokalemia and achlorhydria with marked anemia and jaundice. The patient died of emaciation, dehydration and bronchopenumonia, and an autopsy was performed. Autopsy examination revealed a hen's egg-sized tumor in the tail of the pancreas with metastases in liver, lungs and lymph nodes. In addition, bronchopneumonia and diabetic nephrosclerosis were present. Histologically, the tumor had the characteristics of an islet cell tumor, and histochemically the tumor cells were positive to Grimelius' stain which revealed non-B-islet cell features. Electron-microscopically, the tumor cells had electron dense round membrane-bounded granules resembling non-B-granules of pancreatic islet cells. With the immunoperoxidase procedure (PAP method), tumor cells nearly almost reacted to anti-vasoactive intestinal polypeptide (VIP) serum, which suggested that the tumor of the present case had the capability to produce VIP.

Achlorhydria↗
PubMed1977

An ultrastructure study on antibody production of the lymph nodes of rats with special reference to the role of germinal centers.

Antibody-containing cells in lymph nodes, expecially in germinal centers, and in thoracic duct of rats sensitized by injection of horseradish peroxidase were studied with the immunoperoxidase method. In the primary response the large germinal center cells began to produce antibody on the 9th day and predominated thereafter in germinal centers ,added by intercellular antibody deposition in its late stage. The "ordinary" medium-sized germinal center cells were a minor component. In the secondary response, the intracellular antibody positivity disappeared immediately after the antigenic rechallenge but reappeared from 6 hours later in the large germinal center cells which further transformed from 48 to 72 hours into the "specifically differentiated" medium-sized germinal center cells that disappeared by 96 hours after the secondary injection to enter the thoracic duct. Antibody-containing cells outside the germinal centers appeared 5 to 6 days after the primary injection and were mainly comprised of plasmocytic cell series which was classified into interfollicular large cells, proplasmocytes and plasmocytes. Some results conflicting with the cytological identity of both cell series were presented.

Animals↗
PubMedDate not supplied

The effect of forskolin on the teratogenicity of methylxanthines in the chick embryo heart.

Interactions between forskolin and methylxanthines, including caffeine and isobutylmethylxanthine (IBMX), in the developing chick embryo heart were investigated. Forskolin, a potent activator of adenylate cyclase, was administered to young chick embryos (Hamburger-Hamilton stage 24) together with caffeine or IBMX at doses where each agent alone caused minimal embryotoxicity. The incidence of malformation in the embryonic chick heart or aorta induced by caffeine (5 x 10(-7) or 5 x 10(-6) mol) and IBMX (1 or 2.5 x 10(-6) mol) significantly increased with coadministration of forskolin (1 x 10(-9) mol). Cardiovascular malformations included ventricular septal defect, double outlet right ventricle, and aortic arch anomalies. These results indicate that forskolin potentiates the teratogenicity of caffeine or IBMX on the cardiovascular system in the chick embryo and suggest that this potentiation may be related to increase intracellular cAMP due to stimulation of adenylate cyclase (forskolin) and inhibition of phosphodiesterase (methylxanthines).

1-Methyl-3-isobutylxanthine↗
PubMedDate not supplied

Programmed cell death in the myocardium of arrhythmogenic right ventricular cardiomyopathy in children and adults.

Arrhythmogenic right ventricular cardiomyopathy (ARVC) is characterized by fibrofatty replacement of the right ventricular myocardium. Recently, the myocardial loss in ARVC has been suggested to be related to apoptosis. However, it is still unknown whether this phenomenon is already established in the myocardium of pediatric cases with this disease. We examined the histopathologic characteristics of the ventricular myocardium in specimens obtained from 10 patients, including 3 children with ARVC, and investigated the occurrence of apoptosis in the myocardium by terminal deoxyribonucleotidyl transferase-mediated dUTP-biotin nick-end labeling (TUNEL) assay and agarose-gel electrophoresis of DNA. Endomyocardial biopsy specimens from the 10 cases and a necropsy sample from one adult case with ARVC were examined. Histopathologic examination of biopsy specimens from the pediatric cases revealed extensive fibrosis. Typical fatty infiltration was demonstrated in one of the 3 pediatric cases. These findings were similar to those in adult cases; the histopathologic index based on the severity of myocardial damage, including myocyte degeneration and fibrosis, was not significantly different from that in adult cases. TUNEL assay revealed positive reactivity of the myocardial cells. The apoptotic index was 1.4 +/- 0.4% in children and 1.6 +/- 0.5% in adults (difference not statistically significant). Agarose-gel electrophoresis of a DNA extract of the myocardial tissue of the autopsy case revealed DNA fragmentation. Cases with idiopathic ventricular tachycardia and control cases with a cardiac transplant (with no rejection) had minimal histopathologic findings and negative reactivity in the TUNEL assay. These results indicate that myocardial damage is already established in cases diagnosed as ARVC in childhood, and suggest that the myocardial damage is closely related to apoptosis in children, as well as in adults, in this disease.

Adolescent↗