PubMed Health⌕ Search

Biomedical subjects

T Kawaguchi

Publications and source records attributed to T Kawaguchi.

At least 19 recordsLinked to original sources
PubMed1979

Effect of diuresis on urokinase excretion rate.

UK excretion rate in the diuretic state was investigated in human and animal studies. Loading of water, beer or diuretics in man decreased the UK excretion rate as urine flow increased. A linear relation between UK excretion rate and urine specific gravity was observed. Simultaneous loading of water and exercise suppressed the decrease in UK excretion rate and the increase in urine flow. In animal studies, a null value for UK excretion rate was observed as a result of giving physiological saline and/or furosemide, and a remarkable increase in urine flow was induced. These results clearly suggest the existence of a relation between renal function and UK excretion rate.

Animals↗
PubMed1979

Further studies on the electrophoretic pattern of albumin in diabetic sera.

Human sera from normal subjects and diabetic patients were electrophoresed in an urea-containing gel; polyacrylamide gel and cellulose acetate membrane. In polyacrylamide gel, normal serum exhibited the fast migrating Band 2 and the slower migrating albumin Bands 4 and 5 after overnight fasting and also after glucose administration. In diabetic serum Bands 4 and 5 did not appear before or after glucose administration in low mercaptoethanol gel which abnormality resembled the pattern in C57BL/KsJ-db/db mice. In high mercaptoethanol gel, about half of the diabetic sera exhibit a delay in appearance of Bands 4 and 5, i.e., Bands 4 and 5 were not observed 30--60 min after glucose administration, which seems to resemble the pattern in C57BL/6J-ob/ob mice. Conditions of electrophoresis in urea-submerged cellulose acetate membrane (species of buffer systems, pH, ion concentration, mercaptoethanol, ethylene-diaminetetraacetic acid, Ca2+ ion, urea concentration, etc.) were observed in relation to albumin sub-separation. At pH 8.6 with barbital buffer, albumin separated into two bands, and at pH 10.6 with glycine buffer, albumin separated into four bands. Almost all diabetic sera (ca. 80%) exhibited different electrophoretic patterns from that of normal serum.

Animals↗
PubMed1979

Behavior pattern of rat ascites tumor cells arrested in liver sinusoids: an electron microscopic study.

The behavior pattern of tumor cells in the development of hematogenous liver metastasis by Yoshida sarcoma and two strains of rat ascites hepatoma, AH7974F and AH13, in Donryu strain rats was investigated by electron microscopy. Tumor cells of every strain in liver sinusoids stretched their cytoplasmic protrusions toward the vascular wall and were actually in contact with the endothelial cells with their tips to form various types of junction-like structures. The most distinct one was characterized by parallel and straight arrangement of the opposing cell membranes across a gap of about 100 A. The endothelial discontinuity was easily produced by the projection of cytoplasmic processes and the compression of tumor cells. Through the endothelial defect, tumor cells of Yoshida sarcoma and AH13 migrated locomotively into the dilated spaces of Disse or directly toward the hepatic cells, while AH7974F cells were frequently covered by elongated endothelial cytoplasm. Destruction of the hepatic cells by AH7974F and AH13 cells was found to begin with the insertion of their cytoplasmic processes into the coated vesicles in the hepatic cells. From these investigations, the particular interaction between tumor and normal cells seems to play an important role in the development of liver metastasis by these tumors.

Animals↗
PubMed1979

Tumor formation of rat ascites hepatoma cells in the traumatized brain.

The effect of brain trauma on the formation of metastatic growth was examined by iv injection of 6 strains of rat ascites hepatoma. Three of the tumors (AH7974F, AH66F, and AH13) were in the single-cell state and three (AH272, AH7974, and AH601) contained cell aggregates. None of the tumors showed any tumor growth in the intact brain after injection into the tail vein. However, after traumatization of the brain tissue it was possible to observe tumor formation in the lesion, mainly by injection of the single-cell type tumors. The brain tissue was most susceptive to the tumor cells 10 min and 7 days after trauma. On the other hand, after the injection into the carotid artery of traumatized animals, the tumor formation in the brain was found in both tumor groups.

Animals↗
PubMed1978

Abnormal electrophoretic pattern of albumin in diabetic sera.

Human sera collected from normal subjects and diabetic patients were electrophoresed in an urea-containing gel. The albumin fraction separated into several bands. In normal fasting serum the fast-migrating Bands 1 and 2 were observed and the slower-migrating Bands 4 and 5 did not appear. After glucose administration Band 1 disappeared and Bands 4 and 5 appeared for the first time. In diabetic serum Bands 4 and 5 did not appear before or after glucose administration and this abnormality resembles the pattern in C57BL/KsJ-db/db mice but not C57BL/6J-ob/ob mice.

Diabetes Mellitus↗
PubMed1978

The nature of human blood group A3 erythrocytes.

A population of human erythrocytes (free A3 cells) which was entirely unagglutinable with anti-A hemagglutinins was successfully separated from human blood group A3 erythrocytes by affinity chromatography on a column of lima bean anti-A hemagglutinin (LBH)-Sepharose. The hemagglutinating and the binding properties of free A3 cells with purified LBH and purified eel serum anti-H hemagglutinin (ESH) were investigated. It has been revealed that there exists a bulk of H antigens on free A3 cells, whereas the number of A antigens on a free A3 cell is only 9% of that on an A1 cell. However, no appreciable difference was observed in the binding constants of A1 and free A3 cells to LBH and ESH. From these results it is assumed that the structure of the A antigens on free A3 cells is identical with, or at least similar to, that on A1 cells, but the density of the antigens on the surface of free A3 cells is too low to induce the agglutination of the cells with LBH or anti-A serum.

ABO Blood-Group System↗
PubMed1977

Relationship between transcerebral passage of tumor cells and brain metastasis.

Quantitative study was carried out on transcerebral passage of tumor cells using Yoshida sarcoma and 6 strains of rat ascites hepatoma. The counting was done every second for 40 sec. In relation to the results obtained, histological study was made on the cerebral metastasis of these tumor cells. Metastatic foci in the brain parenchyma, except the meninges and choroid plexus, were formed by island-forming strains (AH-130, AH-272, and AH-7971) which showed comparatively low passage rate and not be single-cell strains (Yoshida sarcoma, AH-7974F, AH-66F, and AH-13) which showed comparatively high rates. Accordingly, it is obvious that there is a correlation between transcerebral passage rates and brain metastasis formation. The mechanisms of metastasis formation were discussed with special reference to arrest of tumor cells in blood vessels.

Animals↗