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T Kitazaki

Publications and source records attributed to T Kitazaki.

28 records · Page 2Linked to original sources
PubMed1983

Changes in the profiles of rodent plasma lipoproteins and apolipoproteins after cholesterol feeding.

Profiling of plasma lipoprotein distribution and apolipoprotein analysis were carried out semi-quantitatively by strictly controlled gel-filtration and SDS slab-gel electrophoresis for rats (Sprague-Dawley rats and ExHC rats), mice and hamsters. The effects of cholesterol feeding on lipoprotein and apolipoprotein patterns of these animals were also studied. HDL were major lipoproteins in all the animals. Hamsters had the most abundant lower density lipoproteins among the rodents with the ratio of cholesterol content between high density lipoproteins and lower density lipoproteins being about 1:1. After cholesterol feeding, the cholesterol content of lower density lipoproteins increased in all the animals. The increase was the greatest in ExHC rats and the least in mice. The profiling methods employed should be of value in determining the changes in the lipoprotein and apolipoprotein patterns of both experimental animals and man.

Animals↗
PubMed1983

Different uptake in rat peritoneal macrophages of beta-very low density lipoprotein prepared from Sprague-Dawley rats and hypercholesterolemic rats (ExHC rats).

Rat beta-migrating very low density lipoprotein (beta-VLDL) was obtained from both ExHC rats and Sprague-Dawley rats which had been fed a cholesterol diet for 4 days, and was incubated with peritoneal macrophages from both kinds of rat to compare the cellular uptake of these lipoproteins. ExHC rat beta-VLDL was taken up by the cells three to five times as well as Sprague-Dawley rat beta-VLDL. Uptake depended solely on the lipoproteins used and not on the cells. Both lipoproteins seemed to be slightly different from each other in apolipoprotein composition. This stimulated uptake may explain the aortic lipid deposits found in ExHC rats with dietary hypercholesterolemia.

Animals↗
PubMed1981

Hypercholesterolemia in ExHC rats and lipid-lowering drug: a screening method for new hypocholesterolemic agents.

The ExHC rat strain which was selected and bred from the Sprague-Dawley strain, develops severe hypercholesterolemia and is liable to aortic lipid deposition when the rats are fed an atherogenic diet. After only 4 days on the atherogenic diet, hypercholesterolemia was induced in these ExHC rats. Following a study of gel-filtration and SDS-disc electrophoresis, significant increases of broad-LDL (beta-VLDL) and of apo E and apo B were noted. On the other hand, plasma HDL and its major apolipoprotein, apo A-I, showed decreases. All these changes might be responsible for the future induction of aortic lipid deposition. A screening method for new hypocholesterolemic agents which takes advantage of these ExHC rat characteristics has been set up; it involves the evaluation of cholesterol lowering and HDL increasing effects by the determination of plasma cholesterol and by radioimmunoassay of apo A-I, respectively. Although clofibrate (100 mg/kg body weight) reduced plasma cholesterol levels significantly, there was no apo A-I increasing effect.

Animals↗
PubMedDate not supplied

Autoimmunity to heat shock protein 40 in ulcerative colitis.

Autoantibodies against heat shock protein 40 (HSP40) and their clinical significance in ulcerative colitis (UC) have not been evaluated before. Twenty-six tissue specimens of inflamed areas from patients with UC, 16 from patients with Crohn's disease (CD) and 16 endoscopically normal tissues were analysed for HSP40 expression. Sera from 47 patients with UC and 44 healthy volunteers were examined for the presence of autoantibodies against HSP40 by enzyme-linked immunosorbent assay test. Immunohistochemistry showed that 17 out of 26 specimens from UC patients, one specimen from a CD patient and one normal tissue specimen were positive for HSP40. Most HSP40-positive cells expressed CD68. Higher titres of anti-HSP40 autoantibodies were detected in sera from UC patients compared with healthy volunteers. In patients with inactive disease, those with proctitis or left-sided colitis had higher titres of anti-HSP40 autoantibodies than those with total colitis. Our study suggests that autoimmunity against HSP40 may have a beneficial effect in UC patients by limiting the extent of the disease.

Adolescent↗