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T Kunitomo

Publications and source records attributed to T Kunitomo.

34 records · Page 2Linked to original sources
PubMed1985

A new form of amyloid protein associated with chronic hemodialysis was identified as beta 2-microglobulin.

Amyloid fibrils were isolated from amyloid-laden tissue obtained from a chronic hemodialysis patient with carpal tunnel syndrome. After solubilization in guanidine HCl, a significant amount of the protein was located in a homogeneous low molecular weight fraction. The protein was found to be identical to beta 2-microglobulin, with regard to its molecular weight of 11,000, amino acid composition and 16 amino-terminal amino acids: Ile-Gln-Arg-Thr-Pro-Lys-Ile-Gln-Val-Tyr-Ser-Arg-His-Pro-Ala-Glu-. These results demonstrate that the amyloid associated with chronic hemodialysis contains as major component a new form of amyloid fibril protein that is homologous to beta 2-microglobulin.

Amino Acid Sequence↗
PubMed1983

Ocular inserts for controlled release of antibiotics.

Ocular inserts impregnated with antibiotics (erythromycin and erythromycin estolate) which have sustained release characteristics were prepared, mainly for the purpose of trachoma therapy. In vitro experiments showed that the elution rate of a drug with low solubility in water (erythromycin estolate) is constant when the water content of the hydrogel insert is more than 30%. In the case of a drug with higher solubility (erythromycin), the elution rate depends on the water content. Some in vivo experiments using rabbit eyes were also reported.

Animals↗
PubMed1983

Phosphate in dialysis patients.

A single pool extracellular compartment (10-15 L) model was demonstrated to fit changes in plasma Pi (at steady state or rebound during HD) on the assumption that its G increased linearly or exponentially during HD. G at the end of HD was estimated to be 30-300 times that at the start of HD, which suggested rapid outflow of Pi from its reservoirs. When bovine or human RBC as a cellular model of probable Pi reservoirs were incubated with solute-free NSS or dialyzed plasma, BUN instantaneously reached a new equilibrium, but Pi and CR gradually flowed out from RBC. Pi efflux from RBC was observed even after the Pi concentration in the incubation media exceeded its initial plasma concentration. With regard to BUN and Cr, the concentration in RBC estimated from that in the incubation media decreased exponentially during HD corresponding to the change in their plasma concentrations. Estimated concentration of Pi in RBC, however, remained unchanged during HD in spite of significant decreases in its plasma concentration. These results suggest that Pi does not leave RBC by simple diffusion and that RBC contains Pi precursor(s). 31P NMR spectra obtained from packed RBC showed that uremic RBC contained more ATP and unidentified compounds (possibly sugar phosphates) than nonuremic RBC and that the peak amplitude of the latter unidentified compounds significantly decreased in uremic RBC leaving the dialyzer or at the end of HD. Furthermore, it was demonstrated that during incubation at 37 degrees C with NSS or dialyzed plasma, the peak amplitude of 2, 3-DPG decreased in contrast with an increased Pi peak.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1982

Dynamic behavior of plasma phosphate in chronic dialysis patients.

1. Change in the plasma P concentration during intra- and interdialytic phases is notably different from that for BUN, CR and UA. Reduction rate for P depends on its pretreatment concentration. Plasma P is apt to level off or rebound even during a treatment and quickly returns to the pretreatment level after it is terminated. 2. In short-term evaluations, occurrence of P rebound during a treatment does not correlate with factors such as meal, A1 gel, dialyzer type, dialyzer membrane and therapeutic mode, but with the pretreatment P concentration. Once it reaches a threshold level inherent to each patient, plasma P seems to rebound. 3. Pretreatment P concentration in each patient seemed to be controlled in a relatively narrow range. 4. While apparent generation rates (G) estimated with a single compartmental kinetic model are stable during intra- and interdialytic phases as to BUN, CR and UA, G for P seems to be significantly enhanced especially during the latter period of the treatment and immediately after the termination of the treatment.

Adult↗
PubMed1981

Spontaneous rupture of the spleen in acute myeloid leukemia.

The results of an autopsy of an 80-year-old Japanese male with acute myeloid leukemia who died of spontaneous rupture of the spleen are reported. The patient was admitted because of anorexia, fatigue, weight loss, and multiple skin eruptions. Hematological examinations indicated a rapid increase in myeloblasts. The patient collapsed on the 28th hospital day, immediately after complaining of severe epigastralgia and vomiting. He died ten hours later. The autopsy revealed extensive leukemic infiltration of the bone marrows, spleen, lymph nodes, skin, and other internal organs. The spleen was enlarged and was ruptured in places at the hilar portion. Massive intraperitoneal hemorrhage from the rupture was the direct cause of death. The mechanisms of splenic rupture are discussed.

Aged↗
PubMed1979

Pseudomyxoma peritonei of ovarian origin--an autopsy case.

The autopsy case of an 80-year-old female with pseudomyxoma peritonei arising in the left ovary is reported. The patient was admitted with complaints of anorexia, sense of fullness and abdominal distension of two months' duration, and died of intestinal obstruction four months later. The autopsy revealed extensive tumor dissemination over the entire peritoneal cavity without any visceral invasion or distant metastasis. A part of the sigmoid colon showed marked stenosis and perforation with abscess formation. Histologically, the tumor was composed of various sized multiple cysts which were completely or incompletely lined by a layer of mucin-producing columnar epithelial cells with moderate nuclear hyperchromatism, and of a papillary pattern in some parts, indicating low grade malignancy.

Aged↗
PubMed1978

High Rates of controlled ultrafiltration combined with optimal diffusion: recent advances in hemodialysis technique.

A system which permits high rates of middle molecule removal while preserving the removal of low molecular weight substances is described. It consists of a high fluid flux hollow fiber artificial kidney and an ultrafiltration controller. When used in combination, high rates of convective transport can be achieved through rapid ultrafiltration and reconstitution of blood with physiologic salt solutions. The theoretical impact of changing from conventional dialysis to this form of therapy on the body burden of middle molecules is estimated.

Diffusion↗
PubMed1977

Controlled ultrafiltration (UF) with hemodialysis (HD): analysis of coupling between convective and diffusive mass transfer in a new HD-UF system.

A convertible and versatile dialysis system consisting of a PMMA hollow fiber unit and UFR controller is shown to be useful for evaluating the effect of solute and fluid removal rates on adequacy of dialysis. Post-dilution mode where high UFR is applied to the patient with matching or comparable dilution rate is concluded to be most effective modification for enhancing the clearance for "middle molecules", without sacrificing the removal rates for the small solutes.

Creatinine↗
PubMedDate not supplied

Performance and clinical use of a convertible hemodialysis (HD) - ultrafiltration (UF) system.

A hemodialysis-ultrafiltration system has been developed and evaluated. It may be used either as a conventional hemodialyzer or as a nondialytic ultrafilter. When used as a dialyzer an ultrafiltration controller is required to fix ultrafiltration rate at the desired level. Rates of low molecular solute removal are compatible with those observed in commercially available artificial kidney systems, higher clearances are obtained for middle molecules, and the rate of ultrafiltration can be controlled within narrowly prescribed limits.

Blood Flow Velocity↗
PubMedDate not supplied

Removal of aluminum from chronic dialysis patients by administration of desferrioxamine and dialysis.

In order to perform quantitative in vitro and clinical studies on the removal of Al by the combined therapy of DFO administration and HD, an HPLC system for measuring DFO was established and a leaky membrane with enhanced permeability to middle molecular substances was developed. The DFO infusion caused rises in plasma Al levels (regarded as indicating the elution of accumulated Al) in patients undergoing HD. Plasma levels increased most in long-term patients and those with bone pain. Examination of Al clearances demonstrated that this increased plasma Al content passed through HD membranes at levels of more than 80 micrograms/l, and that the leaky membrane was more effective for removal of Al as well as DFO than the conventional one. Although a 1:1 complex between DFO and Al in an aqueous solution was confirmed, the formation of Al-rich complexes in vivo was suggested. It is concluded that Al-DFO complex formed in vivo can be effectively removed across the leaky membrane.

Aluminum↗
PubMedDate not supplied

Molecular cloning of the immunodominant regions of hepatitis C virus type III and IV by immunoscreening.

We have identified the immunodominant regions of hepatitis C virus (HCV) type III and IV by immunoscreening of a lambda gt11 cDNA library, which was constructed with RNA extracted from pooled sera of patients. In addition to the nucleocapsid protein, nonstructural region 3 (NS3), and NS4 regions, we found that the region at the N terminus of NS5 in type III and IV were also immunoreactive as well as in the prototype and type II, although amino acid homologies were approximately 60% between the prototype (or HCV type II) and HCV isolates of type III or IV. This result indicated that the region at the N terminus of NS5 may be a candidate for serotyping studies of HCV. This report presents the primary structure of immunodominant portions at the N terminus of NS5 in two HCV subtypes and a preliminary study of the immune responses to these antigens.

Amino Acid Sequence↗