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T Kuno

Publications and source records attributed to T Kuno.

At least 181 records · Page 10Linked to original sources
PubMed1981

Identification of the D-1 dopamine receptor subunit in rat striatum after photoaffinity labeling.

When rat striatal membranes, photolabeled with [3H]dopamine under assay conditions similar to those used for dopamine-sensitive adenylate cyclase, were subjected to sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis, several radioactively labeled bands appeared. Labeling of these bands was reduced in the presence of non-radioactive dopamine during photolysis, but was unaffected by the presence of sulpiride. Haloperidol preferentially reduced the labeling of the main band which had a molecular weight of about 57,000 rather than the other weakly labeled bands. Labeling of this 57,000 dalton protein was not apparent when rat cerebellar membranes were used and was markedly eliminated by kainic acid-induced lesions that destroyed the intrastriatal nerve cell bodies. These results indicate that this 57,000 dalton protein is the binding subunit of the D-1 dopamine receptor.

Animals↗
PubMed1981

Regulation of vitamin B12 and bacteriochlorophyll biosynthesis in a facultative methylotroph, Protaminobacter ruber.

The regulation of vitamin B12 and bacteriochlorophyll formation was studied in a facultative methylotroph, Protaminobacter ruber, classified as a non-photosynthetic bacterium. Vitamin B12 was formed at an almost constant level under various cultivation conditions, while bacteriochlorophyll synthesis was drastically influenced by the growth conditions. Addition of B12 and hemin to the medium and the change of culture conditions from light to darkness in the early growth phases stimulated the pigment synthesis, but no pigment was formed under the continuous illumination and the addition of chloramphenicol inhibited the pigment formation. delta-ALA synthase and delta-ALA dehydratase activities were demonstrated in P. ruber. delta-ALA synthase formation was induced when cultures were transferred from light to dark conditions, which promoted the bacteriochlorophyll formation. Bacteriochlorophyll-protein complex had an absorption maximum at 870 nm and a very small peak at 800 nm.

5-Aminolevulinate Synthetase↗
PubMed1980

Immunohistochemical localization of cyclic nucleotides in guinea pig lip.

The cellular localization of cAMP and cGMP in the guinea pig lip was investigated with an indirect immunofluorescence technique. cAMP was distributed both in cell membranes and cytoplasmic elements with coarse and granular intranuclear fluorescence, while cGMP was localized in nuclear sites including nuclear membranes as well as cell membranes and cytoplasm. In the basal portion, both cAMP and cGMP fluorescence was weaker in cytoplasm and cell membranes than seen in the upper portion.

Animals↗