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T Kunori

Publications and source records attributed to T Kunori.

26 records · Page 2Linked to original sources
PubMed1979

Polyclonal antibody secretion induced in human mixed lymphocyte cultures.

In mixed leucocyte culture (MLC), using human spleen cells or thoracic duct lymphocytes, antibody secretion was induced, measured as plaque-forming cells (PFC) in a haemolysis-in-gel assay with fluorescein isothiocyanate (FITC)-coupled sheep erythrocytes (SRBC) as targets. Peak antibody secretion was seen on day 5. Using protein-A-coupled SRBC as targets and developing antisera, antibody secretion in MLC was found to be of IgM, IgG and IgA type. There was no correlation between the number of PFC against FITC-SRBC in MLC and DNA synthesis. Supernatants from MLC failed to induce antibody secretion.

Antibody Formation↗
PubMed1979

Antibody production and DNA synthesis of human lymphocyte subpopulations induced by PPD tuberculin.

Purified protein derivative (PPD) of tuberculin was found to induce antibody secretion and DNA synthesis in human lymphocytes from blood, spleen, tonsil and lymph node. Antibody secretion was measured as plaque-forming cells (PFC) against fluorescein isothiocyanate (FITC) coupled sheep red blood cells (SRBC) in a haemolysis-in-gel assay. Peak antibody secretion by 100 micrograms/ml of PPD was usually seen on day 6 for blood lymphocytes, and varied from day 3 to day 6 for spleen cells. Peak DNA synthesis for blood lymphocytes stimulated by various concentrations of PPD ranged from day 4 to day 7. The highest number of PFC in tonsil and spleen cells was induced by PPD compared to Staphylococcus aureus bacteria Cowan 1, lipopolysaccharide (LPS) and pokeweed mitogen (PWM). Antibody secretion by PPD was not affected when phagocytic cells were removed by iron treatment. PPD stimulated a higher DNA synthesis in unseparated lymphocytes or mixtures of T and B cells than in enriched T or B cell suspensions. PPD did not induce PFC in B cells enriched by the removal of sheep erythrocyte rosette-forming cells (E-RFC). However, more PFC were stimulated by PPD in enriched E-RFC compared to unseparated lymphocytes, which may indicate that most of the FITC-SRBC reactive B cells also form rosettes with SRBC.

Antibody Formation↗
PubMed1978

Optimal conditions for polyclonal antibody secretion and DNA synthesis in human blood and spleen lymphocytes by lipopolysaccharide.

Lipopolysaccharides from E. coli (LPS) stimulated increased DNA synthesis and high-rate antibody secretion in human lymphocytes from blood and spleen. Peak DNA synthesis by 10 or 100 microgram/ml LPS was seen on day 6 for blood lymphocytes. Using 500 microgram/ml of LPS the peak response was reached on day 5 and with 0.1--1 microgram/ml the maximal stimulation was on day 7. For spleen cells LPS 100 microgram/ml gave a peak stimulation on day 4 or 5. Antibody secretion, measured as plaque-forming cells (PFC) against fluorescein isothiocyanate-coupled sheep erythrocytes in a haemolysis-in-gel assay, was maximal on day 5 in blood lymphocytes and on day 3 or 4 in spleen cells. The highest cell viability and antibody secretion after stimulation with LPS was obtained in 16 mm diameter multiwell plates usng 2.5 x 10(6) cells in 1 ml of medium containing 10% AB serum absorbed with SRBC. Feeding of cultures was essential for stimulation of blood lymphocytes but not for spleen cells.

Antibody Formation↗
PubMed1978

Reactive lymphocyte blastogenesis in canine renal transplantation.

Reactive lymphocyte blastogenesis was investigated by 3H-thymidine uptake of peripheral lymphocytes. Canine renal allotransplantations were performed in 19 dogs, which were divided into three groups by different immunosuppressive therapies. The first group, not treated with any immunosuppressants, showed the highest 3H-thymidine uptake, but the third group, treated with prednisolone (1.5 mg/kg/day) and azathioprine (4 mg/kg/day), did not show remarkable 3H-thymidine uptake except for 2 dogs which died of severe acute rejection. Single use of prednisolone (second group) was less effective in suppression of blastogenesis compared with the combined use of azathioprine and prednisolone. In addition to these experiments, the effects of surgical operation, blood transfusion, and infection on lymphocyte blastogenesis were investigated. Renal autotransplantation did not produce remarkable blastogenesis, but blood transfusion and infection caused strong lymphocyte blastogenesis. Periodical increases of 3H-thymidine uptake were observed in the infected animal.

Animals↗
PubMed1978

Identification of T-lymphocytes in human renal allografts and urine by the fluorescent antibody technique using anti-human T-lymphocyte serum (AHTS).

Immunopathological investigations of the small round cells infiltrating into the tissues of human renal allografts and also those in urinary sediments were performed, using anti-human T-lymphocyte serum (AHTS). AHTS made from ALS is highly specific for T cells, but not for B cells. Tissues obtained by needle biopsy were examined by immunofluorescent staining and conventional histopathological methods. Urinary sediments were obtained from centrifugated fresh urine. Sediments were checked by immunofluorescent and Giemsa staining. Fluorescent positive cells were seen in the interstitial tissues and occasionally in renal glomeruli. Clustered cell-debris in the lumen of renal tubules was also stained by AHTS. In the urinary sediments, about a half of the lymphoic cells were stained by AHTS. The relationship between lymphocyturia and numerous infiltrating cells in the grafts during the rejection crisis has not been clarified. In this experiments, we tried to identify the detailed process of excretion of T cells to urine from renal tissues, using immunopathological methods.

Animals↗
PubMed1977

Some properties of rabbit anti-human thymus serum.

A highly specific anti-human T lymphocyte serum (AHTS) was obtained by sequentially absorbing rabbit anti-human thymocyte serum (ATS) with human red cells, neutrophils, Burkitt's lymphoma cells (Daudi cells), liver, kidney and glutaraldehyde insolubilized human serum. The immunofluorescent staining revealed that the basement membrane of human renal glomeruli reacts with AHTS before being absorbed with kidney. Specificities of AHTS were studied by the cytotoxicity test, inhibition of PHA responses, inhibition of E-resulting and immunofluorescent staining.

Animals↗
PubMed1977

Quantitative studies o the activities of anti-human tonsil sera and anti-human spleen sera against B lymphocyte.

Anti-human B lymphocyte serum (AHBS) was obtained by absorbing rabbit anti-human tonsil serum (ATOS) or rabbit anti-human spleen serum (ASPS) with human red cells, liver, neutrophils, thymocytes and glutaraldehyde insolubilized human serum. Specificities of AHBS were checked by the cytotoxicity test, inhibition test of EAC-rosetting and inhibition of PWM responses. Raji cells of Burkitt's lymphoma originating from B-cells and acknowledged as non-immunoglobulin bearing on the cell surface were stained with AHBS according to the immunofluorescent technique. ATOS showed a higher cytotoxic titer against peripheral B-cells and inhibited EAC-rosetting stronger than ASPS did. On the other hand, macrophages in peripheral blood, Kupffer cells in the liver and certain cells of the thymus-medulla were stained with ASPA, but not with ATOS, even after intensively absorbing ASPS with thymocytes.

Antilymphocyte Serum↗