Rapid detection of Acanthamoeba cysts in corneal scrapings by lactophenol cotton blue staining.
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Acanthamoeba↗
Biomedical subjects
T Kuriakose
Publications and source records attributed to T Kuriakose.
Keratitis due to Arthrobotrys oligospora Fres. 1850.
Mycotic keratitis caused by Arthrobotrys oligospora is reported in a 62-year-old Indian male patient. The diagnosis was made by direct microscopic examination, isolation of large quantities of the fungus on multiple media and the response of the lesion to an antifungal compound. This is believed to be the first documented case of keratitis due to an Arthrobotrys species.
Corneal Ulcer↗
Use of lactophenol cotton blue mounts of corneal scrapings as an aid to the diagnosis of mycotic keratitis.
Lactophenol cotton blue (LPCB) mounts of corneal ulcer scrapings were assessed as a diagnostic tool in cases of mycotic keratitis over a period of 15 months. We investigated 568 cases of ulcerative keratitis by microbiological techniques consisting of direct microscopic examination of LPCB mounts and Gram-stained smears as well as culture of material scraped from the ulcer. Fungi were isolated in large numbers on multiple media from the corneal scrapings of 179 patients (culture-proven mycotic keratitis). Direct microscopic examination of LPCB mounts of corneal scrapings yielded positive results in 140 (78%) of 179 culture-positive patients and negative results in 371 (95%) of 389 culture-negative patients. There was a significant difference between the percentage of positive results obtained by LPCB mounts and by Gram-stained smears in the culture-positive cases. The LPCB mount was positive in greater than 80% of cases of keratitis due to Fusarium spp. and Aspergillus spp. The LPCB mount is strongly recommended as a simple, rapid, inexpensive, and sensitive diagnostic technique in cases of mycotic keratitis.
Aspergillus↗
Keratomycotic malignant glaucoma.
Malignant Glaucoma due to Keratomycosis is a devastating and poorly recognised complication occurring in a small percentage of treated patients. It is characterized, in cases of Keratomycosis by a raised tension, uniform shallowing of the anterior chamber and a fungus-exudate-iris mass covering the pupillary area. Three cases of 'Keratomycotic Malignant Glaucoma' are discussed here. Two of these were successfully treated with therapeutic keratoplasty, extracapsular lens extraction and systemic antifungals. The development of malignant glaucoma after a therapeutic keratoplasty which occurred in one case has not previously been reported. All the three cases which developed malignant glaucoma had a pupillary size of 4 mm diameter or less and grew Fusarium from the cornea and anterior chamber.
Adult↗