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T Kurimura

Publications and source records attributed to T Kurimura.

At least 55 records · Page 3Linked to original sources
PubMed1988

Heterotransplantation of argyrophil small cell carcinoma of the uterine cervix integrating HPV16 DNA into nude mice.

Argyrophil small cell carcinoma of the uterine cervix (ASCC) containing human papillomavirus type 16 (HPV16) DNA has been successfully transplanted in nude mice for the first time, and we have designated the resultant cell line as YIK-1. Histology of YIK-1 was similar to that of the original tumor. The original and YIK-1 tumor cells contained argyrophil granules and neurosecretory granules in the cytoplasm, and were immunohistochemically stained positive for neuron-specific enolase, serotonin and chromogranin. Both tumors contained HPV16 DNA in a multiple-copy integrated form. Thus, YIK-1 maintains the characteristics of the original ASCC, and may therefore be useful as an animal system for experimental studies of ASCC.

Animals↗
PubMed1988

[AIDS and human lymphocytes].

HIV infection is no doubt the primary cause of AIDS. But the relationship between HIV and its host lymphocytes is fairly complicated. It has become clear that the host range of the viruses varies according to the clinical stage of the patients from whom viruses were isolated. Besides, the cause or mechanism of cell killing by HIV infection is not well-known. The density of CD4 molecules on the cell surface can not simply explain the phenomena, such as the level of virus growth, killing mechanisms of lymphocytes and host range of the virus. It is quite interesting to analyse which gene of the virus determines the characteristics of the virus.

Acquired Immunodeficiency Syndrome↗
PubMed1988

Ultrastructural localization of concanavalin A receptors in the plasma membrane: association with underlying actin filaments.

Whole-mount cell preparations of cultured rat 3Y1 cells were examined by stereo electron microscopy to identify the ultrastructural localization of concanavalin A (Con A) receptors in the plasma membrane, and to clarify the relationship between Con A receptors and cytoskeletal components. Well spread monolayer cells were extracted with saponin, briefly fixed, and then partially broken open with shearing force to facilitate the introduction of antibodies for identification of actin filaments. Stereo electron microscopy of such treated cells revealed a 3-dimensional image of filamentous structures such as fine filaments, microtubules (MT) and endoplasmic reticulum (ER) in the flattened areas of each cell. Just beneath the plasma membrane were meshworks of actin-containing fine filaments, as identified by an immunogold staining method. Microtubules and ER were observed to be either directly or indirectly associated with this meshwork. The broken open part of each cell exhibited a meshwork of filaments which were associated with the cytoplasmic surface of the plasma membrane. Some of the filaments were connected to the plasma membrane either by their ends or by their lateral surfaces. The localization of Con A receptors was examined by binding colloidal gold-labelled Con A to the surface of fixed, saponin-extracted cells. Virtually all gold particles bound externally at the same membrane sites where intracellular actin filaments attached internally. The observations strongly suggest that the distribution of Con A receptors was regulated by the underlying meshwork of actin filaments.

Actin Cytoskeleton↗
PubMed1987

Prevalence of antibody to delta antigen among HBV carriers in Japan.

Of 280 hepatitis B virus (HBV) carriers in Kure, Hiroshima Prefecture, Japan, 14 (5.0%) were seropositive for antibody to delta antigen (anti-HD by radioimmunoassay. HBV carriers with chronic liver disease had a greater frequency of seropositivity of anti-HD than asymptomatic HBV carriers (7% vs. 0%, P less than 0.05). Among the various geographic regions of Kure, the east suburban area had a significantly higher prevalence of anti-HD when compared with all other regions combined (9.8% vs. 2.7%, P less than 0.025). It is suggested that hepatitis delta virus (HDV) infection exists in Japan and HDV may be an etiologic agent of chronic liver disease.

Carrier State↗
PubMed1987

Isolation and characterization of a cold-sensitive strain of coxsackievirus A10.

A coxsackievirus A10 strain, isolated from a clinical specimen from a patient with pharyngitis, was characterized with respect to its growth properties in different cultured cells and at different incubation temperatures. This virus multiplied within cultured cells and produced cytopathogenic effects, whereas a prototype strain of coxsackievirus A10 did not. The isolate multiplied efficiently in cultured cells at 37 degrees C but its replication was markedly restricted at 32 degrees C. Temperature shift experiments indicated that the cold-sensitive event affected the late function(s) of the virus.

Cell Line↗
PubMed1987

Maturation of human immunodeficiency virus, strain LAV, in vitro.

The budding process of human immunodeficiency virus (HIV), strain LAV, starts with the formation of a crescent electron-dense layer directly underneath the cell membrane of infected CCRF-CEM cells. After completion of the formation of the circle of inner dense layer, immature virions with an electron-lucent center are released from the cells. Serial thin sections and stereo observation of thick sections showed that most of the immature virions adjacent to the cell surface had already come off the cell and some still had very thin connections to the cell. However, on rare occasions, virions at an intermediate stage between immature stage and mature virions with bar-shaped electron-dense cores were observed. Virions with dense cores were never observed to be connected to the cell surface. These observations support the idea that the last step of the maturation of HIV occurs outside the cell and that the electron-dense core seems to develop by rearrangement and dispersion of the substance of the inner dense layer of immature virions.

Cell Line↗
PubMed1986

Actin filaments and tumorigenicity in a Fischer rat embryo fibroblast cell line (3Y1) transformed by ultraviolet-irradiated HSV.

The characteristics of the cytoskeleton of a Fischer rat embryo fibroblast cell line (3Y1) transformed by ultraviolet (UV)-irradiated HSV were studied by indirect immunofluorescence using anti-actin IgG. Parental 3Y1 cells possessed well-developed actin filaments, while 3Y1 cells transformed by UV-irradiated HSV also retained well-developed actin filaments. Transformed cells were divided into 2 groups according to tumorigenicity in newborn Fischer rats; one had a strongly tumorigenic potential and the other a weakly tumorigenic potential. Tumor-derived cell lines exhibited a highly tumorigenic potential, and were also divided into 2 groups, one with well-developed actin filaments and the other without well-developed actin filaments. Our results suggested that transformation or tumor formation by HSV is a multi-step process and that morphological loss of actin filaments in the cells is not essential to the tumorigenic potential of the cells transformed by HSV.

Actins↗
PubMed1986

Budding process and fine structure of lymphadenopathy-associated virus (LAV).

The budding process and fine structure of lymphadenopathy-associated virus (LAV), were studied by indirect immunofluorescence (IF) and electron microscopy (EM). By IF, LAV antigen was seen to be distributed focally within infected CCRF-CEM cells. Consistent with this finding, electron micrographs showed that LAV particles occurred in a focally aggregated state in a restricted area of the surface of the infected cells. LAV particles possessed bar-shaped, dense and central or eccentric cores. In addition, two or more cores were occasionally observed in one virus particle, or the cores were sometimes absent when thin sections were examined. The envelope of the virus particles had an irregular structure, although LAV particles were approximately spherical.

Antigens, Viral↗
PubMed1986

Characteristic regional prevalence of HTLV-I in Shimane Prefecture.

Serological survey for human T-lymphotropic virus type I (HTLV-I) was performed in Shimane Prefecture, Japan, located on the coast of Japan sea. The rates of anti-adult T-cell leukemia-associated antigen-positive sera were 4.2% and 6.9% respectively for males and females. The rate increased with age, particularly in females. From the geographical point of view, the Oki Islands and a very small focus in Shimane-cho had high rates of anti-adult T-cell leukemia-associated antigen positives. Even within the Oki Islands, there was a big difference in the rate of seropositives between Dozen and Dogo which are only 10 km apart. The focus, Shimane-cho, has a population of 299. Even within a small area, there is a tendency of HTLV-I carriers to form a very narrow focus.

Adolescent↗