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Biomedical subjects

T Kurimura

Publications and source records attributed to T Kurimura.

At least 91 records · Page 5Linked to original sources
PubMed1986

Actin filaments and tumorigenicity in a Fischer rat embryo fibroblast cell line (3Y1) transformed by ultraviolet-irradiated HSV.

The characteristics of the cytoskeleton of a Fischer rat embryo fibroblast cell line (3Y1) transformed by ultraviolet (UV)-irradiated HSV were studied by indirect immunofluorescence using anti-actin IgG. Parental 3Y1 cells possessed well-developed actin filaments, while 3Y1 cells transformed by UV-irradiated HSV also retained well-developed actin filaments. Transformed cells were divided into 2 groups according to tumorigenicity in newborn Fischer rats; one had a strongly tumorigenic potential and the other a weakly tumorigenic potential. Tumor-derived cell lines exhibited a highly tumorigenic potential, and were also divided into 2 groups, one with well-developed actin filaments and the other without well-developed actin filaments. Our results suggested that transformation or tumor formation by HSV is a multi-step process and that morphological loss of actin filaments in the cells is not essential to the tumorigenic potential of the cells transformed by HSV.

Actins↗
PubMed1986

Budding process and fine structure of lymphadenopathy-associated virus (LAV).

The budding process and fine structure of lymphadenopathy-associated virus (LAV), were studied by indirect immunofluorescence (IF) and electron microscopy (EM). By IF, LAV antigen was seen to be distributed focally within infected CCRF-CEM cells. Consistent with this finding, electron micrographs showed that LAV particles occurred in a focally aggregated state in a restricted area of the surface of the infected cells. LAV particles possessed bar-shaped, dense and central or eccentric cores. In addition, two or more cores were occasionally observed in one virus particle, or the cores were sometimes absent when thin sections were examined. The envelope of the virus particles had an irregular structure, although LAV particles were approximately spherical.

Antigens, Viral↗
PubMed1986

Characteristic regional prevalence of HTLV-I in Shimane Prefecture.

Serological survey for human T-lymphotropic virus type I (HTLV-I) was performed in Shimane Prefecture, Japan, located on the coast of Japan sea. The rates of anti-adult T-cell leukemia-associated antigen-positive sera were 4.2% and 6.9% respectively for males and females. The rate increased with age, particularly in females. From the geographical point of view, the Oki Islands and a very small focus in Shimane-cho had high rates of anti-adult T-cell leukemia-associated antigen positives. Even within the Oki Islands, there was a big difference in the rate of seropositives between Dozen and Dogo which are only 10 km apart. The focus, Shimane-cho, has a population of 299. Even within a small area, there is a tendency of HTLV-I carriers to form a very narrow focus.

Adolescent↗
PubMed1986

Sporadic cases of carriers of human T-lymphotropic virus type 1 in Southeast Asia.

Sera obtained from 3,472 persons in Malaysia, Thailand, Philippines and Indonesia were tested for the presence of antibody to adult T-cell leukemia-associated antigen by the gelatin particle agglutination test and indirect immunofluorescence. Among these, only two seropositives were identified. One was a 30-year-old male Malaysian of Indian origin. The other was a 42-year-old female Thai who resided in Bangkok. These results suggested that the infection of human T-lymphotropic virus type 1 might not be endemic in these countries.

Adolescent↗
PubMed1986

Antibodies to human immuno-deficiency virus and human T-cell leukemia virus type I in Japanese patients with hematologic malignancies.

One thousand six hundred and seventy-four blood samples drawn between January 1980 and April 1986 from 1454 Japanese, including 251 leukemia, 409 lymphoma, 76 adult T-cell leukemia and 25 benign lymphadenitis patients, were tested for antibodies to HIV and HTLV-I. No patient with lymphadenitis or lymphoma associated with HIV infection was found. In 87 patients with acute and chronic leukemias who had received multiple transfusions, 8 were positive for anti-HTLV-I antibody after blood transfusions amounting to 115 units, on average, while no patient was positive for anti-HIV antibody. Overall, no sample was positive for anti-HIV antibody, whereas 153 (10.5%) were positive for anti-HTLV-I antibody. These results indicate that the transmission of HIV by blood transfusions is far less prevalent than that of HTLV-I in Tokyo at present.

Acquired Immunodeficiency Syndrome↗
PubMed1986

A novel agglutination test for the human immunodeficiency virus antibody: a comparative study with enzyme-linked immunosorbent assay and immunofluorescence.

A new serological test, the gelatin particle agglutination (PA) test, was developed for the detection of antibody to human immunodeficiency virus (HIV). The sensitivity of the PA test was the same as that of enzyme-linked immunosorbent assay (ELISA) among 761 US and Japanese sera tested. However, false-positive cases were found in 1.2% of all sera in ELISA but in 0% in the PA test (P less than 0.05). There was 100% agreement of seropositivity between PA and a confirmatory indirect immunofluorescence (IF) test. In addition, the agglutination test was reproducible. Thus, the test should be useful for the mass screening of anti-HIV antibody in blood donors.

Acquired Immunodeficiency Syndrome↗
PubMed1986

Prevalence and transmission of human immunodeficiency virus in Japan.

The routes of transmission of human immunodeficiency virus (HIV) can be divided into two categories, 1. sexual transmission (male-to-male, male-to-female and female-to-male) and 2. parenteral transmission by blood or blood products. Among 488 Japanese hemophiliacs, 165 (33.8%) were seropositive and were infected by the injection of factor VIII or factor IX manufactured from American sources. The rates of infection among hemophiliacs were 21.8% (19/87) and 36.8% (43/117), respectively for 1984 and 1985. Sera of 10,272 volunteer blood donors were all negative for anti-HIV. There have been 16 confirmed AIDS cases in Japan consisting of 8 male homosexuals and 8 hemophiliacs, so it can be concluded HIV infections have exclusively occurred among male homosexuals and hemophiliacs. But there are chances to transmit HIV by blood transfusion if the screening of whole blood units is not implemented in the near future.

Acquired Immunodeficiency Syndrome↗
PubMed1985

Neutralizing antibody and interferon-alpha in cerebrospinal fluids and sera of acute aseptic meningitis.

Cerebrospinal fluids (CSFs) and sera from 20 patients with echovirus 30 (E 30) meningitis, 4 patients with enterovirus 71 (EV 71) meningitis, and 5 patients with acute aseptic meningitis (AM) of unknown etiology were investigated at the acute and the convalescent phases of the disease to elucidate the roles of neutralizing antibody (NT) and interferon-alpha (IFN-alpha) in the central nervous system (CNS) in cases of AM in humans. Viruses were isolated from the CSFs at the acute phase of AM, but not at the convalescent phase. There was a fourfold or greater rise in NT titer between paired sera to E 30 or EV 71 but only a slight rise between paired CSFs. IFN-alpha was detected in the CSFs ranging from less than 10 to 25.5 IU/ml but not in the sera, and the IFN-alpha level in the CSF was significantly higher in the acute phase than in the convalescent phase. These results suggest that in cases of acute enteroviral infections in the CNS, NT plays only a small role in the recovery from AM, and IFN-alpha plays a direct or indirect role in curbing the local spread of the virus and eliminating the virus from the CNS at the acute phase of AM.

Antibodies↗
PubMed1985

Survey of the prevalence of AIDS-associated virus (LAV) infection in Japan.

An indirect immunofluorescence method was developed for the assay of antibodies to lymphadenopathy-associated virus (LAV) which is known to be associated with the aetiology of the acquired immunodeficiency syndrome (AIDS). Samples of serum or plasma from 1353 healthy volunteer blood donors, 53 homosexual males, 29 patients who had received multiple blood transfusions and 163 haemophiliacs in Japan were tested for antibody to LAV. Results showed that 47 (29%) of the haemophiliacs, who had been treated largely with factor VIII or IX produced in the USA, were anti-LAV antibody positive, whereas all other subjects were anti-LAV antibody negative. The incidence of antibodies to Adult T-cell leukaemia virus or Human T-cell leukaemia virus I (ATLV or HTLV-I) in these subjects was high.

Acquired Immunodeficiency Syndrome↗
PubMed1985

Oligonucleotide fingerprint analysis of coxsackievirus A10 isolated in Japan.

Eight coxsackievirus A10 strains isolated in 1978 and in 1981 and 1982 from patients with hand, foot-and-mouth disease and with herpangina at a dispensary in Matsue city were compared by RNA fingerprinting techniques. The oligonucleotide maps of the four 1978 isolates were related to each other by 85 to 93% with respect to their large T1 oligonucleotides. In contrast, the oligonucleotide maps of the four 1981 and 1982 isolates were very different from each other. Co-electrophoresis experiments revealed that the 1981 and 1982 strains shared only 17 to 34% of their large oligonucleotides. In addition, some large oligonucleotides were found in most of the fingerprint maps of isolates from 1978 to 1982, suggesting that there are regions in the genome of coxsackievirus A10 which are not subject to mutational changes.

Coxsackievirus Infections↗
PubMed1985

Distribution of the level of antibody to AIDS-associated virus (LAV) in sera from AIDS or AIDS related complex and Japanese hemophiliacs infected with AIDS-associated virus.

The level of antibody to AIDS-associated virus (LAV) in sera from patients with AIDS or AIDS related diseases (AIDS related complex; ARC) and Japanese hemophiliacs was studied using indirect immunofluorescence. Titer of anti-LAV antibody in sera from 89 patients with AIDS or ARC ranged between 10 and 40,960 (median: 1,280) and that of 83 Japanese hemophiliacs ranged from 20 to 20,480 (median: 640). The distribution of the level of anti-LAV antibody in hemophiliacs was similar to that of patients with AIDS or ARC, and no correlation between the titer of antibody and the presence of symptoms of AIDS was observed. Our results suggested that hemophiliacs in Japan might have been infected with live LAV rather than been immunized with inactivated LAV by injection of factor VIII or IX concentrate, and more hemophiliacs in Japan might show symptoms of AIDS in the future as in the United States.

Acquired Immunodeficiency Syndrome↗
PubMed1985

Persistence of hepatitis B e antigen for a long period after the clearance of hepatitis B surface antigen.

Four-hundred and seventy-eight hepatitis B virus (HBV) carriers were followed for more than 60 months. In thirty-six cases the hepatitis B surface antigen (HBsAg) was cleared from serum during the observation period. In one case hepatitis B e antigen (HBeAg) was detectable for more than 66 months even after the appearance of antibody against hepatitis B surface antigen (anti-HBs). HBeAg was detected not only by radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA), but also by reversed passive haemagglutination (RPHA) method which has been believed to be far less sensitive than RIA or ELISA tests. HBV DNA was demonstrated in the serum even after the appearance of anti-HBs. Therefore, it is suggested that HBV (Dane particles) remains or reappears intermittently in the serum after seroconversion of HBsAg antigenaemia to anti-HBs complexes.

Carrier State↗
PubMed1985

[AIDS virus].

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Acquired Immunodeficiency Syndrome↗