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Biomedical subjects

T Kushida

Publications and source records attributed to T Kushida.

At least 73 records · Page 4Linked to original sources
PubMed1980

Morphologic spectrum of liver diseases among chronic alcoholics. A comparison between Tokyo, Japan and Cincinnati, U.S.A.

To deliniate the histopathological features of liver diseases seen in Japanese alcoholics, 130 Japanese alcoholic patients were studied in comparison with 238 American alcoholic patients. In Japan female alcoholic patients were extremely rare. The male to female ratio was 127 to 3 in Japan and 152 to 89 in U.S.A. Although all aspects of alcoholic liver disease did exist in Japan, typical cases of acute alcoholic hepatitis with alcholic hyalins were remarkably smaller in number (9.2% in Japan versus 39.5% in the U.S.A.). The severity based on histopathological findings was also less in the Japanese cases. In contrast to this, 46 percent of Japanese alcoholic patients had chronic hepatitis, and the incidence of multilobular cirrhosis was much greater in Japan wherewith higher prevalence of viral hepatitides.

Alcohol Drinking↗
PubMed1975

Fractionation of whole histone by partition chromatography on Sephadex G-25.

Whole histone from calf thymus was fractionated by partition chromatography on the basis of distribution between an aqueous phase immobilized on Sephadex G-25 beads and mobile organic phases containing various concentrations of trichloroacetic acid. The chromatography was carried out by stepwise elution with five upper phases from water and butanol-2 solvent systems containing 4 M urea and 0.1-1.5% trichloroacetic acid, and finally water. Of the six peaks obtained, two (peaks 1 and 2) contained arginine-rich histones. Although these peaks were still heterogeneous electrophoretically, the band corresponding to F2al was observed only in the electrophoretic pattern of peak 1 and the main fraction in peak 2 was F3. A histone fraction having nearly equimolar amounts of arginine and lysine was obtained from peak 3. Its amino acid composition was similar to that of F2a2. Slightly lysine-rich histone obtained from peak 4 showed an amino acid composition typical of F2b. Peak 5 contained a histone fraction with a ratio of lysine/arginine of 6.14, showing a single band on gel-electrophoresis. Very lysine-rich histone (F1) was obtained from peak 6, and the electrophoretic pattern of this fraction showed a single band.

Amino Acids↗