PubMed Health⌕ Search

Biomedical subjects

T L Huff

Publications and source records attributed to T L Huff.

10 recordsLinked to original sources
PubMed1986

Removal of lipopolysaccharide from acellular Bordetella pertussis vaccine by detergent treatment.

Protective antigen was extracted from Bordetella pertussis cells with 1.0 M NaCl and precipitated with ammonium sulfate, 20-40% saturation (designated fraction 15A-1B). The protective antigen was purified further by detergent (Emulphogene BC720) treatment and adsorption to aluminum hydroxide gel (designated fraction 15A-108A). Compared with B. pertussis vaccine and fraction 15A-1B, fraction 15A-108A retained protective activity as assessed by the mouse protection test, but had reduced protein and markedly reduced endotoxin content. Fraction 15A-108A also had reduced leukocytosis-promoting, histamine sensitizing splenomegaly-inducing, and adjuvant activities. Emulphogene treatment provided a relatively simple method for removing endotoxin from a potential acellular B. pertussis vaccine.

Adjuvants, Immunologic↗
PubMed1986

Role of interferon in streptococcal infection in the mouse.

In previous studies, we have shown the rapid in vitro induction of IFN gamma from human T cells by highly purified peptic extracts of M proteins from Streptococcus pyogenes. The present report extends these in vitro studies and shows that a mixture of both alpha/beta and gamma IFN were present in spleen cell homogenates after in vivo treatment with M protein wild-type (M+) or mutant (M-) S. pyogenes strains. The levels of bacterial-induced IFN were found to be greater in M+ treated animals. Additional studies in vivo showed that pretreatment of mice with heat-killed M+ S. pyogenes organisms significantly protected mice to pneumococcal infection compared to similarly treated M- or control animals (P less than 0.001). Further, antibodies to mouse IFN alpha/beta and antibodies specific to a synthetic N-terminal peptide of mouse IFN gamma enhanced the death of animals due to pneumococcal infection and blocked the protection observed in animals previously treated with heat-killed M+ organisms. Most importantly, treatment of mice with either type of IFN alone enhanced the survival of mice to levels similar to that observed by treatment with M+ organisms (P less than 0.05). The results strongly suggest that IFN can play a crucial role, directly or indirectly, in controlling infection by Streptococcus pneumoniae and perhaps other streptococci.

Animals↗
PubMed1985

Verification index to ensure soldering accuracy.

A technique was presented for the accurate orientation of removable dies with the following advantages: maintains an accurate, intraoral soldering relationship, verifies the accuracy of a soldered FPD, permits resoldering when required, and saves time and patient visits.

Dental Soldering↗
PubMed1985

Evaluation of incisal guide calibrations.

The incisal guide calibrations of three adjustable articulators were investigated and compared to determine the significance of the different incremental markings of each. A method to use vertical changes in the anterior incisal guide of these three articulators accurately to measure jaw and tooth separation was proposed. The relationship between pin opening and incisor opening was evaluated by direct measurement and calculation. Twenty sets of diagnostically mounted casts were examined to evaluate the accuracy of the radius that generates each curved pin in relation to incisor position. Considering the as-manufactured incisal guide markings, casts mounted in a Denar D5A or TMJ articulator will bear a closer relationship between pin opening and incisor separation than casts mounted in a Stuart gnathological computer. To approximate linear incisor separation per calibration mark for each instrument, a table of values was presented for casts mounted at various positions.

Calibration↗
PubMed1983

Construction of composite slides.

This article describes a method for constructing composite slides without destroying the original slide. A conventional slide projector, rear-projector screen, modified 35 mm camera, and slide-duplicating film are required for this technique.

Audiovisual Aids↗