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T L Xia

Publications and source records attributed to T L Xia.

7 recordsLinked to original sources
PubMed2001

Evaluation of germ-cell kinetics in infertile patients with proliferating cell nuclear antigen proliferating index.

AIM: To explore the usefulness of proliferating cell nuclear antigen proliferating index (PCNA PI) in the pathological diagnosis and treatment of male infertility. METHODS: Testicular biopsy specimen obtained from 48 cases of male infertility and 2 normal controls were fixed and embedded. The sections were stained with anti-PCNA monoclonal antibodies or haematoxylin/eosin. Proliferating index (PI), expressed as the percentage of germ-cell nuclei positively stained with PCNA antibody, was assessed from more than 20 seminiferous tubules or 600 germ-cells. RESULTS: The infertile patients were divided into 4 groups: Group 1, normal spermatogenesis (14 cases); Group 2, hypospermatogenesis (16 cases); Group 3, germinal arrest (10 cases); Group 4, Sertoli cell only syndrome (8 cases). The PCNA PI of normal control testis was 86.5% (mean value). Group 3 had a significantly lower PCNA PI (29.8%) than normal testis; Group 1 and 2 had similar PIs (82.3% and 82.3%, respectively) as the control testis. PI of the negative control (Group 4) was 0 as no germ-cells were found. CONCLUSION: PCNA PI is useful for assessing germ-cell kinetics, especially for pathological diagnosis of germinal arrest which is difficult to differentiate by routine HE staining technique. In germinal arrest, there is a significantly lowered PCNA PI, which is an indication of DNA synthesis deterioration, suggesting the use of therapies be different from those for hypospermatogenesis.

Adult↗
PubMed1994

Preliminary study of the frequency of benign prostatic hyperplasia and prostatic cancer in China.

OBJECTIVES: This study determined the frequency of benign prostatic hyperplasia (BPH) and cancer of the prostate (CaP) in China. METHODS: Prostate specimens from 321 unselected autopsies were collected from 1989 to 1992. Slices were cut vertically every 0.5 cm from apex to base. Five to 12 slices were obtained from each prostate. Specimens were stained with hematoxylin-eosin and Masson trichrome. Sixty surgical specimens obtained from cystoprostatectomies with intact prostate were included to determine the frequency of latent CaP. RESULTS: The frequency of BPH, by age, was as follows: 41 to 50 years, 13.2%; 51 to 60 years, 20%; 61 to 70 years, 50%; 71 to 80 years, 57.1%; 81 to 90 years, 83.3%. The frequency of latent CaP, by age, was as follows: 41 to 50 years, 2.2%; 51 to 60 years, 9.3%; 61 to 70 years, 5.9%; 70 years or older, 25%. Incidental CaP was found in 4.9% (33 of 676) of BPH surgical specimens. The incidence of and mortality from CaP in Beijing were 2.41 per 100,000 men and 1.19 per 100,000 men, respectively, between 1985 and 1987. CONCLUSIONS: BPH was rare in China in the early years of this century, but it has become a common disease in recent decades. The histologic frequency of BPH in China was similar to that in Western countries, but the histologic frequency of latent CaP was less than half that in Western countries. The incidence of and mortality from CaP in China are about 20 times less than those in Western countries. Histologic CaP in a Chinese man is not as likely to evolve into clinical CaP as in a Western man.

Adolescent↗
PubMed1994

[Pathological basis for elevation of serum prostate specific antigen].

67 patients with serum prostate specific antigen (PSA) over 4ng/ml were investigated pathologically. All the patients had prostatic lesions: prostate cancer (24) and benign prostatic lesions (43). The serum PSA was conspicuously higher in the carcinoma group than in the benign group (P < 0.01). When 10ng/ml was used as the low limit to detect prostate cancer, the sensitivity and specificity were 83.3% and 74.4% respectively. We suggest that the range of serum PSA from 4.0 to 10.0ng/ml should be considered dangerous in detecting prostate cancer. The epithelium-blood barrier lesion and epithelial hyperplasia of the prostate might be the pathological basis for the elevation of serum PSA.

Adult↗
PubMed1994

[Established oneself the detection of serum prostate specific antigen and application in clinical practice].

Serum prostate specific antigen (PSA)-the biotinavidin enzyme immunoassay (BA-ELISA) was established and used in clinical practice. We assayed serum PSA in normal control and prostatic patients. If 10ng/ml was taken as the cutoff value, the positive rate of prostatic cancer was 62.96%. The results of determination showed: PSA serum levels of patients with complicated BPH (acute prostatitis and chronic prostatitis) were conspicuously higher than the noncomplicated BPH. In the BPH group, we simultaneously detected serum levels of PSA by BA-ELISA and Tandem-E PSA Immuno Enzy Metric Assay. The results suggested BA-ELISA technique is more sensitive and more economical than Tandem-E PSA Immuno Enzy Metric Assay for clinical use.

Aged↗
PubMed1992

[A morphometric grading study on the nucleus shape and DNA-content of transitional cell carcinoma of urinary bladder].

Totally 30 cases of TCC of the urinary bladder were analyzed with IBAS-2000 image analysis system. The nuclear area, perimeter, ellipse factor, etc and nucleus DNA content were measured. The morphometric grading was performed with five correlative factors for classification. They are nucleus perimeter, nucleus ellipse factor, DNA SDE, probability of the cells in C6 zone DNA and probability of the cells in C8 zone DNA. The coincident rate between morphometric grading and WHO grading was 88.9% in G1, 66.7% in G2, and 83.3% in G3. The results indicate that morphometric analysis is a valuable tool in the objective grading of bladder carcinoma.

Carcinoma, Transitional Cell↗
PubMed1989

[Cytologic diagnostic value of voided urine in 60 cases of primary transitional cell carcinoma of the renal pelvis and ureter].

Exfoliative urinary cytology was performed on 60 cases of histologically proven TCC (Transitional cells carcinoma) of the renal pelvis and ureter. There were 39 cases of TCC of the renal pelvis. Urine cytology was positive in 27 cases (69.2%); suspicious in 5 cases (12.8%) and negative in 7 cases (17.9%). Significant correlation was found in the frequency of diagnosis and the histological grade of carcinoma. The cytological positive rate in G1 carcinoma was 40%; 71% in G2; 100% in G3. It is considered that the location of tumor in the renal pelvis predominates the urinary cytological positive rate. Urine cytology was positive in 3 cases (33.3%) out of 9 cases of the carcinoma seated in the inferior renal calix, whereas 19 cases (86.4%) from 22 cases in other sites of the renal pelvis (P less than 0.05). The positive rate of urine cytology for 21 cases of TCC of the ureter was 42.9%. The results showed that the secondary ureteric obstruction was an influence on the cytological positive rate.

Adult↗