Biomedical subjects

T Lenarczuk

Publications and source records attributed to T Lenarczuk.

2 recordsLinked to original sources

PubMed2001

Application of Prussian blue-based optical sensor in pharmaceutical analysis.

Optical flow-through cell-detector with incorporated transparent chemosensitive layer of Prussian blue has been applied in simple, single-channel flow-injection system for pharmaceutical analysis. The reductant analyte converts the Prussian blue based sensing layer to Prussian white form, and the attendant color change is used for sensing. Discoloration of the film is spectrophotometrically detected at 720 nm wavelength. The flow injection system has been successfully used for selective determination of ascorbic acid in simple and complex pharmaceuticals. The method is free from interferences caused by various ions and active ingredients commonly found in pharmaceuticals. The flow-through sensor is useful for spectrophotometric flow-injection analysis of intensively colored and turbid samples. The results of medicine analysis are comparable to those obtained using reference pharmacopeal method. The analytical system could be also used for determination of cysteine and hydrogen peroxide in medicines.

Ascorbic Acid↗

PubMed2001

Optical biosensors based on Prussian Blue films.

Optical biosensing schemes based on enzymatically modified inorganic/organic transparent films predominately composed of Prussian Blue are demonstrated. The composite film, which is non-electrochemically deposited on a non-conducting support. is used as an optical transducer for flow-through biosensors based on hydrolases and oxidases. Urease and glucose oxidase are utilized as model enzymes. Action of the urea biosensor is based on optical pH sensitivity of Prussian Blue indicator. The glucose biosensor is acting as first-generation optical biosensor based on in situ generated Prussian White transducer for hydrogen peroxide. These simple, single-pass transmission optical biosensors exhibit sensitivity in the millimolar range of concentration. The biosensors are very stable owing to presence of a poly(pyrrolylbenzoic acid) network in the composite material. This organic polymer plays a dual role as a binding agent for inorganic material and as a functionalized support for strong covalent immobilization of enzyme molecules.

Journal Article↗