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Biomedical subjects

T Lie

Publications and source records attributed to T Lie.

At least 37 records · Page 2Linked to original sources

Periodontal awareness, health, and treatment need in dental school patients. I. Patient interviews.

The present study aimed to evaluate the periodontal awareness and the amount of information and periodontal treatment received previously in a group of randomly selected patients scheduled for periodontal treatment in a dental school. A group of 124 patients were interviewed by a dental hygienist before the treatment started. Age, sex, and regularity of dental visits were used as predictors. Around 68% of the patients were not aware of the existing periodontal disease, and 74% had never received any information about the treatment possibilities. Ninety per cent claimed that they had never received any periodontal treatment at all. The modality of periodontal treatment and the extent to which the patients had been instructed in toothbrushing were almost equal in patients who visited the dentist yearly and in emergency patients.

Adult

Abrasivity of toothpastes. An in vitro study of toothpastes marketed in Norway.

The purpose of this study was to measure the abrasivity of 23 toothpastes available on the Norwegian market. Additionally, the pH was registered. The testing was based on The British Standards Institution's specification for toothpastes, using a profilometer technique to evaluate the abrasion. The results showed that the abrasivity ranged from 0.049 to 1.367 relative to a standard reference paste. The products were classified as having 'none/slight', 'medium', or 'high' abrasivity. The pH varied between 3.7 and 10.1. Products with low pH showed evidence of a combined erosive/abrasive effect. For some brands there were statistically significant differences between the fluoride and the nonfluoride version.

Dentifrices

Effects of chlorhexidine gel in a group of maintenance-care patients with poor oral hygiene.

An intraindividual double-blind crossover study was designed in order to see if a 1% chlorhexidine gel used twice a day could substitute for ordinary toothpaste in group of maintenance-care patients with poor oral hygiene. Twenty patients from a student clinic participated, each with a long history of maintenance-care problems. A 4-week test period with chlorhexidine gel or placebo gel was followed by an intermediate period with ordinary toothpaste, and then by another 4-week test period. Plaque-covered surfaces (%), bleeding sites (%) and extraneous staining (color slides) were recorded at each sitting. The results demonstrated that brushing with chlorhexidine gel significantly influenced both the oral hygiene level and the gingival inflammation. The only adverse effect observed was a marked increase in the amount of brown staining, especially on the facial surfaces of the nonsmokers. In this short-term study, the staining was not considered cosmetically unacceptable by the patients. The total amount of staining was always greater in smokers than in nonsmokers.

Adult

Evaluation of the effect on root surfaces of air turbine scalers and ultrasonic instrumentation.

Three air turbine scalers were compared to each other and to an ultrasonic instrument (CAVITRON) on medium and maximum power setting. The amount of remaining calculus, roughness and loss of tooth substance were estimated by means of well-defined index systems (RCI and RLTSI). The time required to clean the test surfaces--marked on proximal root surfaces of mandibular incisors--was also noted. Standardized scanning electron microscope (SEM) micrographs were independently "blind-scored" by three examiners, and total mean scores were calculated. The results revealed significant differences (P less than 0.001) between the instruments with respect to the amount of remaining calculus. There were also significant differences (P less than 0.05) between roughness and loss of tooth substance produced by CAVITRON at maximum power setting and that produced by the other instruments. No differences were found with regard to the time required to clean the test surface (until visually clean).

Dental High-Speed Equipment

Surface coatings on dental cementum incident to periodontal disease. (II). Scanning electron microscopic confirmation of a mineralized cuticle.

Root surfaces, exposed by periodontal disease, were studied after treatment with sodium hypochlorite. Observations of the anorganic specimens demonstrated that substantial changes occur in root surfaces incident to periodontal disease. A mineralized surface coating was seen in all areas of the involved root surface, although local regions occurred where the coating was apparently unmineralized. These findings generally confirmed the results of a previous study where the same specimens were studied without hypochlorite treatment. It is concluded that the coating is probably identical to the dental cuticle, and stems from adsorption of components of the gingival inflammatory exudate to the root surface. The coating may also be the carrier matrix for exogenous cytotoxic substances, previously thought to be located in the cementum. The findings may have implications for the way of treating periodontitis-involved root surfaces.

Calcinosis

Surface coatings on dental cementum incident to periodontal disease. I. A scanning electron microscopic study.

Root surfaces of extracted human teeth with advanced periodontal disease were studied by the apically migrated junctional epithelium, the topography characteristic of uninvolved cementum was frequently masked by a surface coating. The thickness of the coating increased in a coronal direction where it often blended with calculus. The observations indicate that this surface coating may originate from the inflammatory exudate and corresponds to the dental cuticle. The findings also indicate that the coating may be mineralized and may contain exogenous substances. These observations illustrate a need for further study and reexamination of the rationale for removal of tooth substance during scaling and root planing.

Dental Cementum

Replica study of plaque formation on human tooth surfaces.

Plaque formation on buccal tooth surfaces was studied by replica technique, consisting of impressions using low viscosity silicone impression materials and positive models produced in epoxy resins. Bacterial accumulation occurred near the cemento-enamel junction in 6-hr specimens, and subsequently expanded in a coronal direction. This development took place partly by extensions of single layers of bacteria, and partly by a pattern where the colonization was mostly restricted to vertical enamel cracks. Plaque accumulations were also frequently located in abrasion grooves and surface pits in the enamel, and prolific plaque areas were consistently surrounded by a monolayer of bacterial cells. Globular and hemispheric structures which occurred, especially on root surfaces immediately after cleaning, were probably artefacts caused by air bubbles or remaining moisture. In separate series of experiments it was demonstrated that improved reproduction of details from the plaque could be achieved by repeating the replicating procedure. The findings indicate that plaque formation starts by adsorption and proliferation of individual bacteria on tooth surfaces, and not by adsorption of aggregates of cells. Special attention should be directed against the problem of artefacts and moisture in replica studies of dental plaque.

Dental Impression Materials

Morphologic studies on dental plaque formation.

The present summary and the following papers (I--VII) are submitted in partial fulfillment of the requirements for the degree of Doctor Odontologiae at the University of Bergen. 1: Growth of dental plaque on hydroxyapatite splints. A method of studying early plaque morphology. J. Periodontal Res. 1974, 9, 135--146. II: Pellicle formation on hydroxyapatite splints attached to the human dentition: Morphologic confirmation of the concept of adsorption. Arch. Oral Biol. 1975, 20, 739-742. III: Scanning and transmission electron microscope study of pellicle morphogenesis. Scand. J. Dent. Res. 1977, 85, 217-231. IV: Early dental plaque morphogenesis. A scanning electron microscope study using the hydorxyapatite splint model and a low-sucrose diet. J. Periodontal Res. 1977, 12, 73-89. V: Ultrastructural study of early dental plaque formation. J. Periodontal Res. 1978, 13, 391-409. VI: Dental plaque morphology as revealed by direct observation and by replicating techniques. Acta Odontol. Scand. 1978, 36, 279-288 Co-author: F. Gusberti. VII: Replica study of plaque formation on human tooth surfaces. Acta Odontol. Scand. 1979, 37, 65-72. Co-author: F. Gusberti. These papers are referred to as I-VII in the present summary. Other papers are referred to in conventional manner.

Dental Impression Technique

Dental plaque morphology as revealed by direct observation and by replicating techniques.

The ability of four elastomer impression materials to reproduce details of bacterial plaque structures was studied by comparing areas of the replica models with corresponding areas of the replicated plaque. Plaque was grown on hydroxyapatite splint segments for 48 hours and replica models were made from three different resins. One of the resins, a methacrylate material, was not suited for this purpose due to its content of spherical particles. The polysulfide and polyether impression materials reacted with the heat curing epoxy resin, and less satisfactory results were obtained with cold curing epoxy resin. Best results were obtained by a combined use of low viscosity silicone impression materials and cold or heat curing epoxy resins as model materials. However, a considerable loss of detail occurred in the replica models compared with observations of plaque directly on splint surfaces. Outlines of individual bacteria could sometimes be seen in the models, but generally several cells seemed to fuse, and appeared like small globular structures where cells and pellicle were difficult to distinguish. The bacterial colonization started near the gingival border of the surface, initiated by attachment of individual bacteria to the pellicle surface.

Dental Impression Materials

Root surface roughness in response to periodontal instrumentation studied by combined use of microroughness measurements and scanning electron microscopy.

Root surface roughness, resulting from calculus removal by hand curette, ultrasonic curette and rotating diamond or Roto-Pro points, was examined by combined use of microroughness measurements and scanning electron microscopy (SEM). Mandibular incisors were placed in a jaw model with a rubber "gingiva" in an attempt to simulate a clinical situation. After cleaning with the various instruments, the surface roughness was measured by an industrial profilometer followed by SEM evaluation. A microroughness index (MRI) was developed and showed that the instruments could be separated into three groups, where the hand curette produced least roughness, followed by the Roto-Pro instrument, while the ultrasonic curette and the diamond left the most uneven surfaces. A SEM roughness index (SRI) based on defined criteria, corresponded well with the results from the profilometer measurements, except that using this index the diamond scored higher than the ultrasonic curette. This was probably due to considerable calculus remnants remaining in the ultrasonic specimens. Characteristic features of the root surface morphology of the cleaned teeth are described. It is concluded that the combined use of microroughness measurements and SEM is of considerable value in this type of studies since more information may be obtained than by any of these methods used separately.

Dental Calculus

Calculus removal and loss of tooth substance in response to different periodontal instruments. A scanning electron microscope study.

The efficiency of calculus removal and the amount of lost tooth substance resulting from the use of hand curette, ultrasonic curette and rotating diamond and Roto-Pro points were examined by scanning electron microscopy (SEM). Root surfaces of mandibular incisors placed in a jaw model were cleaned of adhering deposits, photographed by SEM and evaluated at a standard magnification by three examiners. Scores were given for remaining calculus (RCI) and loss of tooth substance (LTSI) according to defined index criteria. The results revealed that calculus removal was most complete following diamond instrumentation, whereas the ultrasonic curette was least efficient in this respect. Hand curette, ultrasonic curette and Roto-Pro removed about the same amount of tooth substance whereas the diamond removed considerably more than the others. The diamond regularly removed all the cementum and left deep instrumental marks in the dentin; it seems advisable to avoid the use of such instruments in periodontal depuration.

Curettage