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Biomedical subjects

T M Feltkamp-Vroom

Publications and source records attributed to T M Feltkamp-Vroom.

At least 37 records · Page 2Linked to original sources
PubMed1977

Cell-bound IgE on human mast cells and basophilic granulocytes in atopic and nonatopic subjects.

In previous studies basophil-bound IgE was measured by means of quantitative immunofluorescence miscroscopy. But mast cells are of particular importance in local atopic reactions, we thought it necessary to investigate the IgE-load on both cell types. The amount of mast cell-bound IgE can be estimated in a semiquantitative way by end point titration with a fluoresceinated sheep antihuman IgE (FlaIgE). The amount of mast cell-bound IgE was estimated this way in tonsils, adenoids, bronchial and tracheal tissues, and was compared with the amount of IgE on the basophils. A correlation was found between the IgE-load on mast cells and on basophils. Atopic patients had a higher mast cell fluorescence titer (MCFT) than nonatopic controls. It was demonstrated that, as on basophils, the IgE level of the serum contributes to this difference.

Basophils↗
PubMed1977

The absence of a human thymus lymphocyte antigen (HTLA) on bosophils and mast cells.

Basophilic granulocytes and mast cells of different species have been reported to originate from thymocytes and other lymphocytes. These observations were recently confirmed when evidence was given that thymic antigen is present on rabbit basophilic granulocytes. In the study reported here, human leukocytes were tested by the immunofluorescence technique and the immunoelectron-microscopy technique to ascertain whether a human thymus lymphocyte antigen (HTLA) could be detected on their surface. We could demonstrate the presence of HTLA on T lymphocytes but not on basophilic granulocytes, nor on mast cells in Cryostat sections of various tissues.

Adenoids↗
PubMed1977

Immune complex detection by immunofluorescence on polymorphonuclear leucocytes.

Polymorphonuclear leucocytes (PMN) from patients with systemic lupus erythematosus (SLE) were isolated from defibrinated and heparinized blood. In addition, PMN from a healthy donor were incubated with sera from SLE patients and with sera containing artificially prepared immune complexes of hepatitis B surface antigen (HBsAg) and human anti-HBsAg immunoglobulin (anti-HBs) with well defined variations of the antigen/antibody ratio. To one group of blood samples, 5 mM monoiodine acetic acid (MIAA) was added to block in vitro phagocytosis. The Pmn were examined for the presence of IgG, IgM, and HBsAg by the immunofluorescence technique. PMN from defibrinated blood of SLE patients showed in up to 80% immunoglobulin (Ig)-inclusions. However, addition of 5 mM MIAA reduced the number of Ig-containing PMN to at most 40%, which levels were equal to numbers found in specimens from heparinized blood. Addition of 5 mM MIAA to heparinized blood did not reduce the number of PMN with Ig inclusions. Normal donor PMN isolated from defibrinated, heparinized, and EDT blood showed equal amounts of Ig inclusions after incubation with SLE sera, but none when MIAA had been added. In PMN incubated with HBsAg-anti HBs immune complexes with an antigen antibody ratio between 5 and 0-2, both HBsAg and IgG could be detected. It is concluded that Ig inclusions in PMN from heparinized blood from SLE patients are due to in vivo phagocytosis, presumably of circulating immune complexes. In vitro phagocytosis of Ig from SLE sera by normal donor PMN also suggests the presence of immune complexes. Dependent on the antigen-antibody ratio, artificial HBsAg/anti-HBs immune complexes can be detected by in vitro phagocytosis by PM.

Antigen-Antibody Complex↗
PubMed1977

The absence of a human thymus lymphocyte antigen (HTLA) on basophils and mast cells.

Basophilic granulocytes and mast cells of different species have been reported to originate from thymocytes and other lymphocytes. These observations were recently confirmed when evidence was given that thymic antigen is present on rabbit basophilic granulocytes. In the study reported here, human leukocytes were tested by the immunofluorescence technique and the immunoelectron-microscopy technique to ascertain whether a human thymus lymphocyte antigen (HTLA) could be detected on their surface. We could demonstrate the presence of HTLA on T lymphocytes but not on basophilic granulocytes, nor on mast cells in Cryostat sections of various tissues.

Antibodies↗
PubMed1976

The localisation of hepatitis B antigen and immunoglobulin G in liver tissue: an immunofluorescence, light and electron microscopic study.

Liver tissue specimens for 43 HBAg seropositive patients and 22 HBAg seronegative individuals were investigated for the presence of HBAg with the immunofluorescence technique. Histological diagnoses were established with light microscopy on routinely stained frozen liver sections. The liver specimens of 42 out of the 43 HBAg-positive patients showed positive fluorescence. Three distinct fluorescence patterns could be observed: diffuse, focal and solitary. The diffuse pattern appeared to occur particularly in chronic aggressive or persistent hepatitis, the focal pattern was seen only in the group of patients without obvious alterations and the solitary pattern especially in acute hepatic necrosis.

Animals↗
PubMed1976

Tubuloreticular structures and antinuclear antibodies in autoimmune and non-autoimmune diseases.

Tissues obtained at random from patients suffering from autoimmune and non-autoimmune diseases were studied for the presence of tubuloreticular structures (TRS). It could be demonstrated that the occurrence of TRS in renal tissue is not a specific characteristic of systemic lupus erythematosus whereas the presence of such structures in skin tissue might be suggestive for this disease. The serum of some of the patients could be studied for the presence of antinuclear antibodies (ANA). A statistically significant correlation was found between TRS and ANA in the group of patients with autoimmune diseases. The possibility is discussed that this correlation might favour the theory that viruses may be involved in the aetiology of autoimmune diseases, particularly of systemic lupus erythematosus.

Antibodies, Antinuclear↗
PubMed1976

Identification of basophilic granulocytes in human leukocyte suspensions subjected to immunofluorescence procedures with anti-IgE.

A method is described by which human leukocyte preparations obtained by Ficoll-Isopaque centrifugation are stained and fixed in such a way that the basophilic granulocytes in these preparations can be identified when the latter are subjected to immunofluorescence procedures with anti-IgE. Capping, blocking and absorption experiments confirmed the specificity of the test.

Antibodies, Anti-Idiotypic↗
PubMed1976

Analysis of the lympho-plasmacytic infiltrate in Crohn's disease with special reference to identification of lymphocyte-subpopulations.

Lympho-plasmacytic infiltrates in cryostat sections (resected small intestine or colon specimens and rectal biopsies) from 29 patients with Crohn's disease (CD) were studied with the immunoperoxidase and immunofluorescence technique, by means of specific anti-human lymphocyte globulin (ALG) and specific anti-human T-lymphocyte globulin (ATG). Control specimens were obtained from 16 patients with ulcerative colitis (UC) and 12 subjects without inflammatory bowel disease. Characteristic transmural inflammatory infiltrates in CD consisted mainly of lymphocytes. A wide variation of the relative T-cell proportion was observed. However, in contrast with UC, abundant numbers of T-lymphocytes in CD were often detected, particularly in the deeper layers of the bowel wall. Furthermore, in serial sections immunoglobulin-containing plasma cells were counted, using specific anti-IgA, -IgM, and -IgG antisera. A significant reduction of the IgA/IgM plasma cell-ratio was found in CD in comparison with UC and controls. Our results indicate that in CD a chronic cellular immune reaction is going on within the diseases gut, involving increased numbers of lymphocytes and particularly T-cells. It remains to be established whether a deficient IgA barrier has to be considered of primary pathogenetic importance.

Adolescent↗
PubMed1976

Identification of T and B lymphocytes in human breast cancer with immunohistochemical techniques.

Cryostat sections of 50 breast cancer specimens and several lymphoid organs were investigated with antisera against human T-lymphocyte antigen, human lymphocytes, and human immunoglobulins using the immunofluorescence technique and the immunoperoxidase technique. These methods proved to make it possible to discriminate between T and B cells in lymphocytic infiltrates in tissue sections. In nearly all mammary carcinomas studied, T cells were found to predominate in and around tumor cell nests. Only the intraductal carcinomas of this series contained a substantial number of B cells in addition to T cells. The presence of T cells indicates that the host-tumor interaction in vivo mall-mediated immune reaction. The role of the B cells found in the lymphocytic infiltrates of the intraductal carcinomas is still a matter of speculation. Moreover, these findings enhance the value of established histologic classifications. These classifications may have to be modified to provide them with a more functional basis.

Adult↗
PubMed1975

Tubuloreticular structures in human lymphoid cell lines. A cell biological study.

Human lymphoid cell lines were studied as an experimental model for the spontaneous or induced occurrence of tuburloreticular structures (TRS). It was possible to induce TRS after culturing the EB-3 cell line with 20 mug/ml bromodeoxyuridine (BrUdR) during 96 h. Starvation, culturing at lower temperature (32 degrees) or inhibition of DNA synthesis did not give rise to the production of TRS. The response to BrUdR could be blocked with 60 mug/ml thymidine but not with 60 mug/ml deoxycytidine. The addition of 5 mug/ml cytarabine or the removal of BrUdR at different times resulted in inhibition of TRS induction, indicating that BrUdR had to be incorporated into DNA during at least 48 h. After incorporation, neither the presence of BrUdR nor DNA synthesis was necessary for the production of TRS. These experiments and the finding that in the cell line IHTC-33, which does not produce Epstein-Barr virus associated antigens, TRS were spontaneously present, exclude a correlation between TRS and these antigens. However, the induction of TRS by BrUdR may be related to the activation of another (latent) virus.

Antigens, Viral↗