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Biomedical subjects

T M Orlova

Publications and source records attributed to T M Orlova.

11 recordsLinked to original sources
PubMed1987

[Adaptation of hepatitis A virus strain (JaM-55) originally pathogenic for monkeys to a cell culture].

The results of adaptation of hepatitis A viral strain JaM-55 to the culture of embryo kidney cells FRhk-4 from macaque Rhesus are presented. The viral strain was isolated from a M. fascicularis suffering from spontaneous hepatitis. Before inoculating the cell culture the virus was passaged twice in the M. arctoides capable of reproducing hepatitis. FRhk-4 cell line inoculation by the monkey liver extract, containing the strain HAV-YaM-55, resulted in isolation of single viral particles of hepatitis A in the preparations obtained at the first 3 passages by the 28-31 day of cultivation. Beginning from the fourth passage the abrupt increase in the number of viral particles and hepatitis A antigen was registered. There were no traces of cytopathogenic effect at any level of viral passages in the inoculated cell culture. The adapted virus contains hepatitis A viral RNA identified by spot hybridization with the cloned cDNA of hepatitis A virus.

Adaptation, Biological↗
PubMed1982

[Spontaneous sister chromatid exchanges in cultured African green monkey cells].

The frequencies of sister chromatid exchanges (SCE) were studied in primary kidney cell culture, diploid cell line 5018, pseudodiploid spontaneously transformed cell line 4647 and hypotetraploid cell line 455 of the African green monkey. Bromodeoxyuridine was used at a concentration of 10 micrograms/ml. The slides were stained with acridine orange and Giemsa for sister chromatid differentiation. In the first three cases, SCE were scored in 100 metaphases of each culture. The distribution of cells from the number of SCE differs from the normal law and was approximated fairly well by the lognormal distribution pattern. The geometrical means of SCE per cell were 6.90 for cells in primary culture, 9.26 for cells of diploid line 5018; 4.50 for cells of pseudodiploid transformed line 4647 and 9.76 for cells of hypotetraploid transformed line 455.

Animals↗
PubMed1980

[Chromosome nucleolus organizer regions of the African green monkey].

The silver staining Ag-I for detection of nucleolar organizer regions (NOR) in the African green monkey chromosomes was used. NOR were situated on the secondary constrictions of one pair of homologous marker chromosomes and consisted of two parts. A block of condensed chromatin was seen in the middle of the despiralized portion corresponding to the secondary constriction. The data suggest that the stalks of satellites of chromosomes, rather than satellites themselves, are regions of nucleolar organizer.

Animals↗
PubMedDate not supplied

[Study of human and simian hepatitis A virus isolated by an immunoenzyme method using polyclonal and monoclonal antibodies].

Hepatitis A viruses (HAV) isolated from man and Old World monkeys varied in their interaction with monoclonal antibodies (MCA) in enzyme immunoassay. All human HAV isolates reacted with the MCA under study (HIC19, H7C27, and K3-4C8), whereas the viruses isolated directly from monkeys did not react with H7C27 MCA and some of them also with H1C19 MCA. Simian HAV strains became capable of reacting with H7C27 MCA and H1C19 MCA after adaptation to cell culture. In tests with human and simian immune sera (polyclonal antibody to HAV) a number of human isolates interacted with human serum antibodies much more intensively and with simian serum antibodies.

Animals↗
PubMedDate not supplied

[Characteristics of electrophoretic types of rotaviruses isolated from children with acute gastroenteritis].

In an analysis of sporadic cases of gastroenteritis in children, rotaviruses were detected in 61 out of 363 specimens examined. Studies of rotavirus isolates by polyacrylamide gel electrophoresis (EP) of RNA revealed 9 different EP types. In one region, during the entire epidemic season one "long" EP type was prevalent. In one patient, a mixture of a "short" and "long" EP types was detected. In another region, "atypical" rotaviruses were found not belonging to the antigenic group A of rotaviruses.

Acute Disease↗
PubMedDate not supplied

[Effect of the antiemetic preparation dimetpramid on rat reproductive function].

It has been demonstrated that dimethpramide exerted an adverse effect on the sexual glands of female rats. It provoked an inhibition of ovarian function (derangement of cycling rhythmicity, the loss of individual cycle stages) and depletion of energy resources of the uterus (drop of glycogen content). The reduction of the gonadotropic activity of the pituitary was also observed.

Administration, Oral↗
PubMedDate not supplied

[Toxicity of an original antiemetic preparation dimetpramid].

A new original drug dimetpramid injected per os into mice and rats exhibits moderate toxicity, exerts a weak irritating action on the mucous membranes. The drug is capable of penetrating via the unaffected skin, producing systemic effects. With all routes of administration the drug may have a moderate inhibitory action on ovarian function and the manifestation of the hypotensive effect.

Animals↗