PubMed Health⌕ Search

Biomedical subjects

T M Vyvial

Publications and source records attributed to T M Vyvial.

8 recordsLinked to original sources
PubMed1975

Methodologic differences in values for M-proteins in serum, as measured by three techniques.

Accurate and reproducible measurement of M-proteins is essential for managing patients with monocional gammopathies, but serum protein electrophoresis, radial immunodiffusion, and electroimmunodiffusion yield comparatively divergent results. We have studied these differences and their causes. Sera from cases of IgG-monoclonal gammopathy, IgA-monoclonal gammopathy, and IgM-monoclonal gammopathy were assayed by each of the three techniques. Results indicated inter-method discrepancies as great as fivefold for all proteins studied. For IgG-monoclonal gammopathy, radial immunodiffusion values were less consistently so. For IgA-monoclonal gammopathy, both radial immunodiffusion and electroimmunodiffusion gave lower results than did serum protein electrophoresis. For IgM-monoclonal gammopathy, results were variable, but values by radial immunodiffusion tended to be higher than, and electroimmunodiffusion comparable to, those for serum protein electrophoresis. The differences were not correlated with protein abundance, serum freshness, immunoglobulin class, light-chain type, ultracentrifugal characteristics, or electrophoretic mobility. Clearly, values for M-protein concentration depend on the techniques used to obtain them. We postulate that subclass differences may contribute to the diversity of radial immunodiffusion results, and that for electroimmunodiffusion the fixed electrophoretic mobility of M-proteins leads to unpredictable results. We conclude that serum protein electrophoresis is the best of the three assay techniques for M-proteins.

Blood Protein Electrophoresis↗
PubMedDate not supplied

Validating the probability of paternity.

In cases of disputed parentage, it is commonly thought that the probability of paternity (P) represents the probability that the tested man is the biologic father of the child whose parentage is in doubt and that the paternity index (PI) is the likelihood ratio that enters into this probability. Using the results of HLA typing in a sample of disputed parentage cases, the distribution of P was examined within two groups: actual mother-alleged father-child trios, and simulated mother-unrelated man-child trios. These distributions were found to be roughly consistent with the usual understanding of P.

Female↗