Biomedical subjects

T Macchia

Publications and source records attributed to T Macchia.

22 records · Page 2Linked to original sources

PubMed1979

[Characterization of an analytical method, comparison between two methods and electronic elaboration of data (author's transl)].

An experimental scheme for the evaluation of two parameters of validity of an analytical method, precision and accuracy, has been reported. Particular emphasis has been given to the problem of comparison between two methods. In the description a validity limit of the scheme has been given and certain critical points, commonly encountered in the measurement of the above mentioned parameters during the preparatory as well as the experimental phase, has been underlined. Further, with the help of tables and graphs, an example for electronic elaboration of the data obtained during the course of standardization of an automatic method for the determination of uric acid in serum according to the above scheme, has been given.

Autoanalysis↗

PubMed1979

[Automatic determination of triglyceride, cholesterol, glucose and serum uric acid in the screening for preventive medicine (author's transl)].

A centrifuge speed analyzer, used for triglyceride, cholesterol, glucose and serum uric acid determinations, was tested as to linearity, precision, steadiness and accuracy requirements. The results were compared to those obtained by other techniques. Automatic techniques were proved to comply with reliability and practicability requirements, necessary during the screenings for preventive medicine. The centrifuge speed analyzer seems to be particularly fit for the primary prevention trials of coronary heart disease, since the follow-up studies require repeated laboratory examinations.

Adult↗

PubMed1979

[Evaluation of smoke consumption by the determination of serum thiocyanate (author's transl)].

The method of Butts and Coll. for determination of serum thiocyanate--parameter correlated to smoke assumption--has been modified in order to improve sensibility and has been characterized as regards precision and accuracy. The improved method has been successively utilized for determination of serum thiocyanate of 493 subjects during a screening for prevention from coronary heart disease. A linear correlation (r = 0,916) between the number of smoked cigarettes and serum thiocyanate has been found. Assuming 40 mumol/l as a cut-off point to distinguish cigarette smokers and non smokers, 1,8% false positive and 5% false negative resulted.

Adult↗

PubMedDate not supplied

Ethanol in biological fluids: headspace GC measurement.

The present study evaluates the suitability of headspace gas chromatography (GC) with a capillary column as a method for determining the ethanol content in different biological fluids. This procedure allows the use of headspace GC not only as a reference method but also in routine diagnostics and monitoring work. The recent literature reviewed reports no standardized methodology that is at the same time suitable for ethanol determination in all routinely available biological fluids (blood, serum, plasma, urine, and saliva). The proposed procedure seems to be a good solution to the problem. The reproducibility study for the biological fluids tested resulted in coefficients of variation that ranged from 0.8 to 2.9% and recoveries that averaged 99%. Linearity was verified in the range of 0.01-20 g/L of ethanol in aqueous solutions. Sensitivity was determined to be 0.01 g/L. Ethanol measurement by this method is easy, simple, and highly reliable, and only a small sample volume (0.1 mL) is required. An internal standard and sample manipulation are not necessary. The obtained results suggest that the use of headspace GC could be extended from confirmatory analyses to routine application in many biomedical fields.

1-Propanol↗