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Biomedical subjects

T Malinowski

Publications and source records attributed to T Malinowski.

10 recordsLinked to original sources
PubMed1998

Preliminary report on the apparent breaking of resistance of a transgenic plum by chip bud inoculation of plum pox virus PPV-S.

Five transgenic clones of Prunus domestica L. containing plum pox virus (PPV) coat protein (CP) gene and one non-transformed control clone were challenged with PPV-S in the field. Symptoms developed on C2, C3, C4, C6 and B70146 but not C5 trees inoculated by chip budding (CBI) (2/2, 2/2, 1/1, 2/2 and 2/2, positive/inoculated) in the first summer after inoculation. However, in the second year, symptoms appeared on CBI C5 trees. The presence of the virus in the plants was confirmed by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) amplification of a fragment of viral polymerase gene. During two years, symptoms of infection developed on 3 to 4 of 8 non-inoculated trees of clones C2, C3, C4, C6 and B70146. Eight non-inoculated C5 trees remained symptomless and ELISA-negative as of spring 1998, in spite of the presence of aphid vectors and inoculum sources.

Animals↗
PubMed1995

Expression of the plum pox coat protein gene in transgenic Nicotiana tabacum plants.

Plant expression vector pBI 121 containing the gene encoding coat protein of Plum Pox Virus of the Skierniewice isolate (CP PPV-S) was prepared (clone pCM1). The construct was used for transformation of Nicotiana tabacum plants using an Agrobacterium tumefaciens based system. About 82% of kanamycin resistant plant lines contained a transgene (the sequence of CP PPV-S) but only 81% of them actively expressed the PPV-S coat protein gene as measured by RT-PCR.

Agrobacterium tumefaciens↗
PubMed1994

Nucleotide sequence of the coat protein gene of the Skierniewice isolate of plum pox virus (PPV).

The coat protein (CP) gene of the Skierniewice isolate of plum pox virus (PPV-S) has been amplified using the reverse transcription--polymerase chain reaction (RT-PCR), cloned and sequenced. The nucleotide sequence of the gene and the deduced amino-acid sequence of PPV-S CP were compared with those of other PPV strains. The nucleotide sequence showed very high homology to most of the published sequences. The motif: Asp-Ala-Gly (DAG), important for the aphid transmissibility, was present in the amino-acid sequence. Our isolate did not react in ELISA with monoclonal antibodies MAb06 supposed to be specific for PPV-D.

Amino Acid Sequence↗
PubMed1991

[Histamine inhalation test in patients with specific bronchial hyperreactivity].

In 46 patients with a clinical suspicion of bronchial hyperreactivity tests of non-and specific bronchial hyperreactivity with the sensitizing allergen were carried out. The results were compared with the results of other allergological tests (skin prick tests, IgE and allergen specific IgE serum levels). The results of this study demonstrate the importance of duration, character and concentration of allergen exposition on formation of specific bronchial hyperreactivity. Non-specific and specific bronchial hyperreactivity demonstrates tissue dependent variations, this being seen especially in seasonal allergens. The authors have also shown a relation between both types of hyperreactivity and the results of other allergological tests.

Administration, Inhalation↗
PubMed1982

[Occupational allergy in miners].

The authors allergologically analysed 141 coal miners afflicted with upper respiratory tract inflammations. In 7% of cases the disease was found to be induced by coal dust. All the subjects exhibited positive dermal tests with common allergens (plants pollens, household dust, saprophytes, moulds). The authors suggest that atopic status may contraindicate the work in coal mining. 57% of cases exhibited changes in the nose inhibiting its patency (hypertrophy of nasal concha, nasal mucosa polyps, lateral deviation of the nasal septum).

Adult↗