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Biomedical subjects

T Manji

Publications and source records attributed to T Manji.

17 recordsLinked to original sources
PubMed1980

Effects of 1 alpha-OH-D3 on bone mineral content in the forearm of chronic hemodialyzed patients.

The bone mineral content in the forearm of 99 male and 62 female patients who had never been treated with vitamin D on hemodialysis was measured to elucidate the relationship between bone mineral content and duration of hemodialysis. We found that reduction in bone mineral content related to the duration of hemodialysis in both sexes. 1--2 microgram/day of 1 alpha-OH-D3 was administered to 35 hemodialysis patients for 2 years. Bone mineral content in the forearm of both patient groups treated with and without 1 alpha-OH-D3 was measured every 3 months. Although bone mineral content in the forearm of the hemodialysis patients decreased, serum Ca, serum-ionized Ca, and serum phosphate levels increased and serum ALK-Pase and serum immunoreactive PTH decreased significantly after administration of 1 alpha-OH-D3. When bone mineral content in the forearm of patients treated with 1 alpha-OH-D3 was compared with that of untreated patients, the loss of bone mineral content in patients treated with 1 alpha-OH-D3 was less than that of the untreated group. In addition to uremic metabolic derangements, intermittently repeated humoral derangement on hemodialysis seems to cause loss of bone mineral content.

Adolescent↗
PubMed1979

Ion-exchange chromatographic separation and fluorometric detection of guanidino compounds in physiologic fluids.

A high-performance liquid chromatographic procedure has been developed for the separation and fluorometric detection of guanidino compounds in physiologic fluids. All guanidino compounds were separated on a 17 X 0.46 cm cation-exchange column using a stepwise pH gradient. The chromatographic system was designed to enable the use of the specific reagent 9,10-phenanthrenequinone as a means of monitoring the guanidino compounds of physiologic fluids. This new analytical method is so sensitive that it enables the analysis at the picomole level. Our automatic guanidino-compound analyzer was successfully applied to the quantitative determination of all guanidino compounds in physiologic fluids from normal controls and uremic patients.

Autoanalysis↗
PubMed1979

Quantitative analysis of methylguanidine and guanidine in physiologic fluids by high-performance liquid chromatography-fluorescence detection method.

A rapid and sensitive high-performance liquid chromatographic method has been developed for the quantitative analysis of methylguanidine and guanidine in physiological fluids. These quanidino compounds are separated on a 6 x 0.23 cm cation-exchange column with 0.5 M sodium hydroxide solution. The guanidino compounds are detected with a fluorometer, which monitors the fluorescent guanidine derivatives produced by the reaction of the eluted constituents with 9,10-phenanthrenequinone. Sensitivity to sub-nanomole levels of methylguanidine and guanidine is demonstrated. The method was successfully applied to physiological fluids such as serum and cerebrospinal fluid from uremic patients.

Chromatography, High Pressure Liquid↗
PubMed1978

Studies on nitrogen and amino acid metabolism in hemodialysis patients using 15N-labelled compounds.

The degree of 15N incorporation into serum albumin studied by 15N-urea administration in dialyzed patients on a 1.3 g/kg/day protein diet was shown to be almost the same as in non-dialyzed uremic patients on low protein diet, while there was no incorporation in a normal subject. 14.1 g of EAA and histidine was intravenously given in dialyzed patients on the high protein diet and improvements in the level of BUN and anemia were observed. The study with 15N-leucine in a patient proved that about 36% of EAA infused during dialysis was transferred into dialysate. AAD was prescribed by giving 15-20 g of EAA, histidine and tyrosine to patients at each dialysis. The AAD enabled us to give a large amount of EAA asymptomatically in a short time, improving anemia and decreasing the BUN level. 15N-glycine administration in a dialyzed patient proved that 15N incorporation into serum albumin was 3 times greater than his non-dialyzed uremic stage on a low protein diet, and that EAA serum concentrations and non-EAA which had not been added into dialysate were elevated.

Adult↗