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Biomedical subjects

T Marík

Publications and source records attributed to T Marík.

30 records · Page 2Linked to original sources
PubMed1983

Unchanged binding of 99molybdenum to red cell membrane proteins in hereditary spherocytosis.

The interaction of 99Mo with red cell membrane proteins was found specific for spectrin both in normal red cells and those of hereditary spherocytosis. In addition, no significant quantitative differences were observed in labeling patterns between these two types of red cells, thus indicating no major alterations in the spectrin molecules of hereditary spherocytosis.

Blood Proteins↗
PubMed1983

Spectrin oligomers of the red cell membrane extracts in hereditary spherocytosis.

Spectrin oligomers were extracted from the erythrocyte membrane at 37 degrees C with the low ionic strength buffer. The spectrin extractability of HS membranes was enhanced compared with controls in some cases. The major spectrin component in the extract was dimer which was, apart from a tetramer, purified by gel filtration on the Sepharose 4B column. No changes of dimer-tetramer interconversion in the extract from HS membranes were observed. Similarly, the protein composition of the membrane extracts from two types of red cells remained unchanged.

Erythrocyte Membrane↗
PubMed1978

A new simple method for determination of erythrocyte filtrability.

A new simple filtration technique designed for measuring red cell filtrability in the routine laboratory use was developed. The suspension of the whole blood in saline (1:20,000 dilution) was processed on the Sartorius filter membranes, pore size 8 micron. The percentage of passed erythrocytes indicating red cell filtrability was determined. The suitability and perspective applicability of this method for studying various hematological disorders is proposed.

Diagnosis, Differential↗
PubMed1977

Immunoprecipitation and radioimmunological studies of a lymph node activating factor released during contact of H-2-or HLA-different lymphocytes.

Immunoprecipitation studies showed that the supernatants from the 4-hour mixed cultures of allogeneic mouse lymphoid cells contained a specific component with the alpha2-globulin electrophoretic mobility which can correspond to, or to which a "lymph node activating factor" can be bound. In addition, during allogeneic cell culture serum proteins with the prealbumin mobility were released into the culture medium and in the control culture of cells from only one donor proteins with the albumin mobility predominated. In the supernatants from the 4-hour cultures of human HLA-identical and HLA-diferent lymphocytes beta2-microglobulin was determined by immunodiffusion and radioimmunologically.

Alpha-Globulins↗
PubMedDate not supplied

[New tests for laboratory diagnosis of spherocytosis].

The authors evaluated advantages of the glycerol lysis test (AGLT) and its modification the "pink" test in the laboratory diagnosis of hereditary spherocytosis (HS). Both tests were positive in all examined subjects with HS and in some patients with autoimmune haemolytic anaemia (AIHA) and were negative in controls and some other haemolytic diseases. As these tests are easy to perform and quick, they can be recommended as a supplementary or screening examination in hereditary spherocytosis. The "pink" test has, as compared with the ALGT test, the advantage that it is simpler.

Glycerol↗