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Biomedical subjects

T Matsuya

Publications and source records attributed to T Matsuya.

At least 73 records · Page 4Linked to original sources

Respiratory activities in relation to palatal muscle contraction.

This study investigated the activities of the tensor and levator veli palatini muscles related to respiration. During quiet breathing, no activity was observed in either muscle. With either hypercapnic or hypoxemic condition, the tensor veli palatini muscle exhibited phasic activity during inspiration. The levator veli palatini muscle showed phasic activity during expiration with hypoxemia (PaO2 less than 40 mm Hg). NaCN perfused bilaterally through the carotid sinus induced these respiratory activities. The tensor veli palatini muscle was more sensitive than the levator veli palatini muscle to NaCN.

Animals↗

Surgical treatment using porous hydroxylapatite blocks for severe habitual dislocation of the bilateral temporomandibular joint in a patient with epilepsy.

A patient with severe habitual dislocation of the bilateral temporomandibular joint involving epilepsy was operated using porous hydroxylapatite blocks as intervention material. The patient was followed up for 4 years. He has been well without recurrence of dislocation or any complication. In this paper, we report the procedure and the relevant literature is discussed.

Dental Care for Chronically Ill↗

Intramuscular hemangioma in the digastric muscle.

Intramuscular hemangioma is a distinctly rare neoplasm originating within normal muscle. In the head and neck region, it occurs most frequently in the masseter muscle. Diagnosis of intramuscular hemangioma is extremely difficult because it is not encountered frequently and is often confused with salivary gland stone, parotid neoplasm or other tumors. In this report, a second case of intramuscular hemangioma of the digastric muscle and the diagnosis of this type of tumor in the early stage and the importance of its treatment are described.

Female↗

Immunohistochemical localization of epidermal growth factor (EGF) and EGF receptor in human oral mucosa and its malignancy.

The immunohistochemical localizations of human epidermal growth factor (hEGF) and EGF receptor (EGFr) in oral tissues, including normal mucosa, leukoplakia and squamous cell carcinoma were examined by the use of monoclonal antibodies to hEGF and EGFr. In normal mucosa and leukoplakia, immunostaining of hEGF was limited to an underlying layer of connective tissue near the epithelium. The intensity of extracellular staining appeared to increase with the degree of epithelial malignancy and was eventually most striking in the stroma of invasive carcinoma. The epithelial cells in normal mucosa, leukoplakia, and squamous cell carcinoma showed negligible immunoreactivity for hEGF. Expression of EGFr appeared to be associated with the proliferative activity of cells and/or epithelial malignancy. In normal mucosa, anti-EGFr monoclonal antibody reacted only with the basal cell layer. In all sections of leukoplakia, the positive cells for EGFr were found in the prickle cell layer in addition to the basal cell layer. Most tumour cells in squamous cell carcinoma were strongly positive for EGFr. These findings indicate increased expression of hEGF and EGFr with malignancy. The characteristic localization of extracellular hEGF in the underlying connective tissue and in stroma of oral mucosal tumours suggests a possible epithelial-mesenchymal interaction in hEGF secretion.

Antibodies, Monoclonal↗

The innervation of the levator veli palatini muscle by the glossopharyngeal nerve.

We developed a novel method which enables bloodless exposure of the levator veli palatini muscle in rat in order to investigate the physiological properties of this muscle. The levator veli palatini muscle which is innervated by a branch of the glossopharyngeal nerve showed rhythmic spontaneous movement in rats. Cutting the branch supplying LVP of the glossopharyngeal nerve caused cessation of the spontaneous movement of the levator veli palatini muscle. The spontaneous discharges of the glossopharyngeal nerve were synchronized with those of the phrenic nerve. A mixture of 95% oxygen and 5% room air influenced the efferent discharges from the branch of the glossopharyngeal nerve supplying the levator veli palatini muscle. These findings indicate that the motor nerve supply to the levator veli palatini muscle is the glossopharyngeal nerve, and the levator veli palatini muscle is related to the respiratory system, in particular with inspiration in rats.

Action Potentials↗

Mechanical contraction property of the levator veli palatini muscle.

Twenty adult dogs anesthetized with sodium pentobarbital were used as experimental animals in an analysis of the mechanical contraction properties of the levator veli palatini muscle. The following results were obtained: the contraction time and half relaxation time of maximal isometric twitch of the muscle were revealed to be 43 msec and 33 msec, respectively; a summation curve was obtained with stimulation at 15 Hz and maximal tetanic tension was at 70 Hz. The average of the tetanic tension ratio was found to be 5.7. The maximum rate of tension rise was observed at 150 Hz, and the value was twice the rate of the twitch contraction. The duration time of the active state plateau showed values of 3.9 to 4.5 msec. The contraction time of the levator veli palatini muscle closely resembles that of the intrinsic laryngeal and the orbicularis oris muscle.

Animals↗

Biological characterization of pseudocyst-forming cell lines from human adenoid cystic carcinomas of minor salivary gland origin.

Two cell lines (ACCS and ACCY) were isolated from two individuals with adenoid cystic carcinoma (AdCC) using tissue culture techniques. Both cell lines have similar morphology, i.e., elongated and flattened cells with slender cytoplasmic processes. The two cell lines tend to form pseudocysts, which are a specific architectural feature of AdCC. Coexpression of cytokeratin and vimentin was found in the two cell lines, which occasionally also contained S-100 protein and lactoferrin or lysozyme immunoreactivity. Moreover, ACCS and ACCY displayed potential for the production of a large amount of extracellular matrix including basal lamina components such as fibronectin, laminin, and type IV collagen and glycosaminoglycans which are also part of the basal lamina. These findings suggest that the tumor cells, probably basal or myoepithelial like cells, are responsible for the formation of the peculiar stroma of AdCC consisting of a large amount of collagen-like fibers, basal lamina components, and mucopolysaccharides.

Carcinoma, Adenoid Cystic↗

Malignant schwannoma and melanoma occurring in the maxilla.

A rare case of malignant melanoma of the hard palate following malignant schwannoma of the left maxilla in a 47-year-old woman is reported. Twenty-two months after surgical excision of malignant schwannoma with postoperative radiation and chemotherapy, malignant melanoma appeared with symptoms of gingival bleeding and swelling. Although the patient was treated by the partial maxillectomy with radical neck dissection and postoperative chemotherapy and adjuvant immunotherapy, she died of multiple distant metastases 9 months after the oral manifestation. A possible histological relationship of both tumors which are considered to be derived from the neural crest cells is also discussed.

Female↗

Factor VII deficiency first observed at preoperative routine clotting test.

Factor VII deficiency was first observed in a 24-year-old female in the routine clotting test for tooth extraction. Her factor VII was 22%, mildly below normal limits. Factor IX complex was not administered as replacement therapy when her impacted tooth was extracted. There was no sign of abnormal postoperative bleeding. As this disease was a rare disorder, the minimum safe level of factor VII for tooth extraction was unknown. We had a opportunity to study a patient with this disorder observed in the preoperative routine clotting test for tooth extractions.

Adult↗

Serum dipeptidyl peptidase activities as a possible marker of oral cancer.

Serum glycyl-l-prolyl 4-methyl-coumaryl-7-amide (gly-pro-MCA) hydrolase (DPP IV) and L-lysyl-L-alanyl beta-naphthylamide (lys-ala-beta NA) hydrolase (assumed to be DPP II) activities were measured in patients with oral squamous cell carcinoma and healthy subjects. The mean serum DPP IV activity of all cancer patients was significantly (P less than 0.001) decreased, compared with that of healthy subjects. Although there was no significant difference between the stages by International Union Against Cancer (UICC) classification (1978), DPP IV levels tended to change dynamically, reflecting the clinical status during therapies. The serum DPP IV activity of patients with a fair prognosis was significantly elevated toward the normal range, whereas the activity of patients with a poor prognosis was significantly decreased (P less than 0.05). In contrast, the mean serum lys-ala-beta NA hydrolytic activity of cancer patients was significantly (P less than 0.001) increased, compared with that of healthy subjects, and was changed reciprocally to DPP IV activity. The correlation of these two serum enzyme activities with tumor weights also was observed in animal models using nude mice transplanted with human KB carcinoma cells and hamsters transplanted with BHK21 cells. These results indicate that these serum enzyme levels may become an aid for the diagnosis of malignant tumors and for estimating the prognosis of the patients.

Adult↗

Different contents of glycosaminoglycans in a human neoplastic salivary duct cell line and its subclone with a myoepithelial phenotype.

The glycosaminoglycans (GAG) biosynthesized by a neoplastic human salivary duct cell line, HSGc, and by its nontumorigenic subclone, HSGc-E1, having a myoepithelial-like phenotype, were examined by incorporation of [3H]-acetate into GAG. The rate of GAG radiolabeling in HSGc-E1 was significantly greater than that in HSGc. The radiolabeled GAG recovered from HSGc-E1 showed a distribution of 22-32% in the cells and 68-78% secreted into the medium, while the amounts of GAG in the cells and medium of HSGc were equal. Two-dimensional electrophoresis of GAG extracted from the cells demonstrated that HSGc-E1 contained a much greater amount of heparan sulfate (HS, 53.5% of total), while HSGc synthesized hyaluronic acid (HA, 17.5%), HS 38.8%, chondroitin sulfate (Ch-S, 27.6%) and dermatan sulfate (DS, 16.1%). Moreover, treatment of HSGc with sodium butyrate or dibutyryl cyclic AMP (each is a potent inducer of differentiation to myoepithelial-like cells) strongly enhanced GAG synthesis, while dexamethasone (an inducer of differentiation to a more functional duct epithelium) did not stimulate GAG synthesis. These findings suggest that biosynthetic changes in the GAG content of neoplastic salivary cells are associated with their myoepithelial differentiation.

Clone Cells↗

Infiltrating lipoma of the tongue.

A case of an infiltrating lipoma, occurring in the tongue of a 56-year-old Japanese woman, is presented. The relevant literature is discussed.

Diagnosis, Differential↗

High induction of poly(ADP-ribose) polymerase activity in bleomycin-resistant HeLa cells.

Poly(ADP-ribose) polymerase activity was measured in bleomycin (BLM)-resistant HeLa (HeLa-BLMr) and the parental HeLa cells after BLM treatment. HeLa-BLMr cells, which had been subcultured in growth medium containing 1 micrograms/ml of BLM, showed a 3.75-fold higher enzyme activity than did HeLa cells, but this activity was decreased to the same level as that of HeLa cells after 48 h of BLM-free cultivation. When HeLa and HeLa-BLMr cells after a 48-h cultivation in BLM-free growth medium were treated with BLM, the enzyme activity was induced at a higher level (2.5-3.8 times) in HeLa-BLMr than in HeLa cells and was inhibited markedly in HeLa-BLMr and slightly in HeLa cells by nicotinamide, an inhibitor of this enzyme. The BLM-induced cell killing by nicotinamide was highly potentiated (about 18 times) in HeLa-BLMr as compared to HeLa cells.

Bleomycin↗

[Radial flap: a valuable fasciocutaneous flap for intra-oral reconstruction].

The radial forearm flap, or the forearm flap, is called "Chinese flap" for its development of the chinese doctors, and is originally designed for the correction to the post-burn contraction of the face and neck. The radial forearm flap is one of the fasciocutaneous flap, supplied by the radial artery, and transferred as a single-stage reconstruction micro-surgically. In oral and maxillofacial region, the deltopectral flap (D-P flap) and the pectralis major myocutaneous flap (P-M-M-C flap) are mainly used for the reconstruction. These flaps, however, are sometimes too bulky and limited to transfer, and more require two-stage operations. On the other hand, as the forearm flap being thin and pliable, some doctors use this flap micro-surgically at single-stage free flap reconstruction. Before two years, we have begun to transfer the radial forearm flap for the intra-oral reconstruction. The operation method is as follows. Design and Elevation of the Radial Forearm Flap 1. Using the ultrasonic doppler flow meter, the radial artery and the subcutaneous forearm veins are marked on the skin. 2. The flap is designed 20% larger according to the pattern to be reconstructed, with the distal section of the radial artery as an axis on the forearm and the median vein of forearm inclusively. 3. Before the operation, Allen test must be performed in order to determine whether the hand will survive without a radial arterial in-put. 4. The operation is performed with a arm tourniquet. The margin of the flap are incised down to the deep fascia, isolating and preserving the proximal subcutaneous veins as required.(ABSTRACT TRUNCATED AT 250 WORDS)

Forearm↗

[A broken needle in the pterygomandibular space].

We encountered a case where a 30 gauge disposable dental needle was accidentally broken and inoculated into pterygomandibular space by a dentist during a block anesthesia for the inferior alveolar nerve. Several approaches including use of a guide needle and roentgenograms were made but all were unsuccessful. Eleven days later, further operation was attempted monitoring the needle on X-ray TV, resulting in that the broken needle was successfully removed. This technique may be useful for removal of mandibular broken needles.

Anesthesia, Dental↗

Human serum ubiquinol-10 levels and relationship to serum lipids.

Serum and urinary levels of ubiquinol-10 (UQH2-10) as well as total ubiquinone-10 (total UQ-10, sum of UQH2-10 and oxidized UQ-10) were determined in healthy Japanese subjects using high performance liquid chromatography with an electrochemical detector. The mean serum and urinary levels of UQH2-10 were 0.75 +/- 0.21 microgram/ml (86% of total UQ-10, n = 77) and 0.045 +/- 0.016 microgram/mg creatinine (59% of total UQ-10, n = 30), respectively. After daily oral administration of 60 mg of UQ-10 for 7 days, the human serum level of UQH2-10 increased twice as compared to that before the treatment. However, the ratio of the UQH2-10 to the total UQ-10 remained unchanged during such administration. The serum level of UQH2-10 correlated significantly with those of vitamin E (p less than 0.001), phospholipids (p less than 0.01), total cholesterol (p less than 0.05), and total lipids (p less than 0.05). There was no correlationship between the serum levels of UQH2-10 and of triglycerides and lipid peroxides.

Adolescent↗

[Chemo-sensitivity of a human salivary adenocarcinoma cell line to several anti-cancer drugs and enhancement of the antitumor effects by combination with filipin or verapamil].

A human salivary adenocarcinoma cell line, HSGc, was tested for the chemosensitivity and compared with a human squamous cell carcinoma cell line, KB. From IC50 values of anti-cancer drugs on the cells, it was found that HSGc was sensitive to CDDP, 5-FU and DTIC but resistant to ADM, MTX, VCR, PEP and MMC as compared to KB. The chemosensitivity of HSGc was in agreement with previously reported clinical data on the therapeutic results of salivary gland tumors. This suggests that HSGc may be an useful model for understanding the biological response of salivary adenocarcinoma cells to anti-cancer drugs. We further examined the combined effects of filipin and verapamil with these anti-cancer drugs. Filipin was found to enhance cell-killing effect of 5-FU and PEP on HSGc, while the combination of filipin with either DTIC or PEP was also effective on KB. Verapamil was effective in combination with 5-FU, VCR or PEP on HSGc and with DTIC, VCR or PEP on KB. Especially the most predominant enhancement on HSGc was observed in combination of filipin or verapamil with PEP. These findings suggest that even low-sensitive drug, PEP, is also useful when combined with either filipin or verapamil.

Adenocarcinoma↗