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Biomedical subjects

T Meshkov

Publications and source records attributed to T Meshkov.

11 recordsLinked to original sources
PubMed1994

[Granulocyte alkaline phosphatase--a biomarker of chronic benzene exposure].

In tracing the cellular population status in the peripheral blood of workers, exposed to benzene, was included and cytochemical determination of the alkaline phosphatase activity in leucocytes. This enzyme is accepted as marker of the neutrophilic granulocytes, as maturation of the cells and their antibacterial activity are parallel to the cytochemical activity of the enzyme. 78 workers from the coke-chemical production from state firm "Kremikovtsi" and 41 workers from the production "Benzene" and "Isopropylbenzene"--Oil Chemical Plant, Burgas are included. The benzene concentrations in the air of the working places in all productions are in the range of 5 to 50 mg/m3. For cytochemical determination of the alkaline phosphatase activity is used the method of L. Kaplow and phosphatase index was calculated. It was established that in 98.4% of all examined the alkaline phosphatase activity is inhibited to different rate, as from 46.5% [61 workers] it is zero. In considerably lower percentage of workers were established and other deviations: leucocytosis or leucopenia, neutropenia, increased percent of band neutrophils and toxic granules. The results of the investigation of the granulocyte population show that from all indices, the activity of granulocyte alkaline phosphatase demonstrates most convincing the early myelotoxic effect of benzene.

Air Pollutants, Occupational↗
PubMed1987

[Thrombocyte functions in malignant blood diseases].

Platelet aggregation with various inductors and platelet secretion were studied in chronic lymphoid leukemia, myeloma, chronic myeloid leukemia and acute leukemia. It was established that disturbed aggregation with adrenaline and disturbed secretion are found more often in these malignant blood diseases. An interesting fact is that there is no correlation between the disturbed platelet functions in vitro and the clinical manifestations of disturbed hemostasis (hemorrhages or thrombosis).

Acute Disease↗
PubMed1981

[Biphasic peroxidase in the study of Fc receptors of leukemic lymphocytes].

The authors examined Fc receptors of cytoplasmic membrane by means of a two-stage immunoperoxidase technique: at the first stage the immuno-gamma-globulins (IgG), labelled with peroxidase, were connected with the membrane receptors, but at the second stage the connection was stabilised by supplementation of homologous antigen. In healthy persons (20 volunteers) the percentage of lymphocytes, which carried membrane Fc receptors for IgG, were in the ranges of 25% and 42%. In leukemic lymphocytes, verified as B population by immunofluorescent technique, they found that over 95% of these lymphocytes carried membrane receptors for Fc of IgG (examined in 10 persons with chronic lympholeucosis). It could be concluded that the leukemic process does not involve Fc receptor structures in contrast to immunoglobulin receptors, which in these cases are altered considerably, having in mind the amount of the connected labelled IgG and the way od its location on the cytoplasmic membrane.

B-Lymphocytes↗
PubMed1979

Comparative ultrastructural morphometrical studies in acute leukemia.

Four hundred paramyeloblasts (from meyloblastic, promyelocytic, monoblastic and lymphoblastic types), isolated from peripheral blood of untreated leukemia patients, were studied by planimetric ultrastructural morphometry. The data of 19 parameters for these four paramyeloblastic types were compared with statistical methods. More central "scattered" heterochromatin was found from this morphometric investigation, i.e. early prophases in the lymphoblastic type of acute leukemia (these cases are more sensitive to therapy). The absolute mean values of the areas of whole cells, areas of the nucleus and nucleolus, areas of the heterochromatin and other indices show that different cell clones will undergo leukaemic transformation.

Cytological Techniques↗
PubMed1978

[Ultrastructural studies of the plasma cells in multiple myeloma].

Electron microscopic investigations of 38 patients with multiple plasmocytoma were carried out, the patients being divided into three groups according to the type of monoclonic immunoglobin in serum or urine. Light to moderate cellular-cytoplasmatic asynchronism was found as well as hypertrophic nucleoli and versatile changes in cytoplasmatic organelles of the poliferous plasmocytes. Cellular-cytoplasmatic asynchronism is discussed as a characteristic sign of neoplastic plasmatic cells. No correlation was found between the morphological changes in the nuclei and cytoplasma and the immunoglobin class, secreted from the cells.

Bone Marrow↗
PubMed1977

Comparative ultrastructural morphometric studies of micromyeloblastoid and lymphoblastoid paramyeloblasts.

One hundred lymphoblastoid and one hundred micromyeloblastoid paramyeloblasts, isolated from peripheral blood of untreated leukaemia patients, were studied by electron microscopic morphometry. Considerable differences are to be found between the micromyeloblastoid and lymphoblastoid paramyeloblasts as regards the size of the nucleolar "apparatus", both in the absolute average values and in the index showing the ratio of the nucleolous of the remaining nuclear surface (4.76% for myeloblastoid and 9.96% for lymphoblastoid paramyeloblasts). The central heterochromatin (scattered), which is discussed to be essential for the detection of an early prophase, was found in 3% of the myeloblastoid cells and in 14% of lymphoblastoid ones. The effect of cytostatic therapy is discussed by taking these data into consideration.

Cell Nucleolus↗
PubMed1974

[Pathologic neutrophilia in acute leukemia].

The morphological hemogram peculiarities are described in 10 patients with acute leukosis (nine with myeloblastoid variance and one with monocytoid variance of acute leukosis). Leukemic hiatus is not to be found in all the patients and besides paramyeloblasts--granular "filamented neutrophils" are present in the peripheral blood in considerable absolute values. Cytomorphologic, cytochemical and electron microscopic investigations were carried out on those "paraneutophils". Complex data for pathological leukosis characteristics of those cells were established that differed them from the normal cells. In five of the patients--definite data for acute leukosis were obtained with the histomorphological investigation of the blood tissue, in that way excluding primary myeloblast phase of myeloleukosis. Conclusions were drawn that those cells ("paraneutrophils") have the same prognostic value in peripheral blood as paramyeloblasts and are not affected by polychemical therapy. This was stressed to be a more particular variance of acute leukosis.

Adult↗