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Biomedical subjects

T Morito

Publications and source records attributed to T Morito.

At least 37 records · Page 2Linked to original sources
PubMed1989

Stimulatory effect of cefodizime on macrophage-mediated phagocytosis.

We evaluated the ingestion of anti-sheep erythrocyte (anti-E) IgG- and IgM-coated sheep erythrocytes by murine peritoneal macrophages exposed to cefodizime, a new semisynthetic cephalosporin, and other antibiotics. Cefodizime enhanced the ingestion of anti-E IgG-coated erythrocytes by peritoneal macrophages from CD-1 and BALB/c mice in a dose-dependent manner, but had no effect on uncoated or IgM-coated erythrocytes. Similar enhancement was observed only in the case of cefpimizole (AC-1370), among the other antibiotics examined. These results suggest that the favorable in vivo activity of cefodizime and cefpimizole may result from their phagocytosis-enhancing as well as antimicrobial properties.

Animals↗
PubMed1989

Studies on anti-liver cell membrane antibodies in sera of patients with autoimmune chronic active hepatitis.

In using the rat hepatocytes isolated and treated with formalin as antigen, IgG class antibodies to the liver cell membrane (LM-Abs) in sera of patients with chronic active hepatitis were examined by both indirect immunofluorescence (IF) and solid-phase enzyme immunoassay (EIA). Specificity of the reaction in IF and EIA was confirmed by an absorption test using different tissues and by inhibition test using the liver specific protein. Indirect IF test using isolated hepatocytes showed the linear staining in eight of 14 sera of patients with autoimmune chronic active hepatitis (CAH). Mean binding values and positive frequency of LM-Abs to the isolated hepatocytes which were detected by EIA were 4.18 +/- 2.99 and 71.4% (10/14) in autoimmune CAH, 3.16 +/- 2.50 and 50% (6/12) in primary biliary cirrhosis, 1.40 +/- 2.12 and 28.6% (4/14) in non-A, non-B, chronic hepatitis (NANB-CH) with serum gamma-globulin showing more than 2.0 gr/dl, 1.02 +/- 1.30 and 0% (0/13) in NANB-CH with gamma-globulin showing less than 2.0 gr/dl, 1.04 +/- 1.20 and 0% (0/15) in HB-CH, and 1.80 +/- 3.60 and 25.0% (3/12) in SLE. Titers of LM-Abs in serum of a autoimmune CAH patient decreased significantly after treatment with prednisolone, but their titers did not correlate with the activities of anti-nuclear antibodies or anti-smooth muscle antibodies. From these results, it was considered that some of NANB-CH patients with both LM-Abs and higher serum gamma-globulin more than 2.0 gr/dl might have some autoimmune characters similar to CAH.

Animals↗
PubMed1989

[An autopsy case of a patient with myasthenia gravis who showed various symptoms of collagen diseases and complicated with malignant thymoma].

A 37-year-old man suffered from photosensitivity and urinary casts with serological findings of positive anti-DNA antibody, LE cells and false positive VD reaction in September of 1979. He developed general fatigue, dyspnea and diplopia with ptosis of bilateral eyelids in November of 1979, which were improved by the anti-cholinesterase drugs. In January of 1980, he had an attack of unconsciousness and his chest X-ray film showed several tumorous shadows in the anterior mediastinum and middle and lower lung fields. Treating him with chemotherapy of VEMP, the pulmonary shadows disappeared. However, he developed severe muscle weakness with an elevated CPK (430 mU/ml) and a myogenic EMG pattern along with an increased anti-acetylcholine receptor antibody (243 n Mol/l), dysphagia and eyelid-ptosis. He died in September of 1985 and his autopsy disclosed a malignant thymoma of mixed type in the anterior mediastinum and an atrophy and fibrosis with infiltration of inflammatory cells in the striated muscles.

Adult↗
PubMed1987

A competitive inhibition test of enzyme immunoassay for the anti-nRNP antibody.

A competitive inhibition test for anti-nRNP antibody was developed, using horseradish-peroxidase-labelled anti-nRNP IgG derived from the serum of a patient with mixed connective tissue disease. In this test, the anti-nRNP antibody was clearly distinguished from the anti-Sm antibody in the patients' sera, and the sensitivity of the assay for anti-nRNP antibody was close to 50 ng/ml.

Adult↗
PubMed1987

Localization of nRNP antigen in mammalian cells stained with peroxidase-labelled IgG fraction of anti-nRNP antibody obtained from a patient with mixed connective tissue disease (MCTD).

The peroxidase-labelled IgG fraction of anti-nRNP antibody obtained from a MCTD patient stained the nuclei of almost any kind of cells of man and rat. In addition, the cytoplasma of anterior horn cells of the rat spinal cord was exclusively reacted with this antibody. Both cytoplasmic and nuclear staining of the anterior horn cells were reduced after treatment of tissue sections with RNase.

Animals↗
PubMed1986

Studies on Hanganutziu-Deicher antigens-antibodies. I. Hanganutziu-Deicher antibodies of IgG class in liver diseases.

Sera of patients with various liver diseases were examined for the presence of Hanganutziu-Deicher (H-D) antibodies by enzyme immunoassay with high-molecular weight glycoprotein (HMWGP) isolated from bovine red blood cell stromata. IgG class H-D antibodies were demonstrated in sera of 5.9% of acute hepatitis, 28.1% of chronic hepatitis and 21.9% of liver cirrhosis patients. H-D specificity of the antibodies under investigation was determined by absorption experiments. Evidence was also presented that the H-D antibodies in the liver disease sera are directed to N-glycolyl neuraminic acid (NGNA) and/or NGNA-dependent determinants of HMWGP.

Antibodies, Heterophile↗
PubMed1985

Dextran and antidextran antibodies in the sera of patients with liver diseases.

Dextran and antidextran antibodies were examined by enzyme immunoassay (EIA) and immunodiffusion in sera of 108 patients with various liver diseases. In EIA, IgG class antidextran antibody was detected in 16 patients (14.8%), mostly with chronic liver diseases such as liver cirrhosis (26.9%) and chronic hepatitis (22.2%). IgM class antibody was detected in 10 patients (9.2%). Results obtained by EIA inhibition revealed that dextran antigen was present mostly in sera of patients with acute liver diseases such as fulminant hepatitis (75.0%) and acute hepatitis (17.2%). Identity of the dextran antigen in the liver diseases serum and the dextran preparation recognized by an antibody-containing serum was demonstrated. These results suggest that the damaged hepatocytes in the process of the liver disease may release the dextran antigen into the patient's circulation which is responsible for the formation of antidextran antibodies by the patients with liver diseases.

Acute Disease↗
PubMed1984

Differential inhibition of lymphocyte function by 2-chloroadenosine.

The effects of 2-chloroadenosine, a poorly metabolized adenosine analogue, on some human lymphocyte functions were studied. Mixed lymphocyte responses were strongly inhibited by very low concentrations of 2-chloroadenosine. The mitogen-induced proliferation of human lymphocytes was also generally suppressed by 2-chloroadenosine in a dose dependent manner. Blastogenesis induced by Con A and PWM was severely inhibited by low doses of 2-chloroadenosine while its inhibition of that induced by PHA was less marked. Natural killer cell activity was inhibited only about 55% by high concentrations of 2-chloroadenosine. These results suggested that many subsets of human lymphocytes are controlled by adenosine receptor.

2-Chloroadenosine↗
PubMed1984

Serological interactions among sera of human renal graft recipients.

Serological interactions among sera of human renal graft recipients in double diffusion in gel were observed by chance. Antigen was detected in six of 127 recipients and antibody in two of 30 recipients tested. One of the six recipients carrying the antigen had also antibody in one serum sample. On the basis of the pattern of reactions observed, the hypothesis was expressed that the described antigen-antibody system had "pan" rather than "allo" character.

Antigen-Antibody Reactions↗