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Biomedical subjects

T N Petrova

Publications and source records attributed to T N Petrova.

At least 19 recordsLinked to original sources
PubMed1996

[Olifen action on mutagenesis in human lymphocytes].

Antihypoxic drug olifen has been studied for effect in 72-hour PHA-stimulated cultures of blood lymphocytes in 15 healthy donors and 35 patients with diseases accompanied by enhanced mutagenesis in lymphocytes (rheumatoid arthritis, systemic lupus erythematosus, hypophyseal adenoma, neoplasms, chronic intoxications). Olifen has no mutagenic effect on mutagenesis in lymphocytes in a wide dose range. In diseases with intensive mutagenesis in lymphocytes olifen exhibits antimutagenic effect without lymphopoiesis.

Adult↗
PubMed1994

[Changes in the cell membrane permeability of chick myoblasts in primary culture when in contact with rat neutrophils].

The reaction of chicken myoblasts in competent primary culture during their contact with activated rat neutrophils was evaluated. In the incubation medium dynamics of activities were defined for proteinases and creatine kinase, protein content, as well as for DNA and protein marked precursor increase. An increase in myoblast membrane permeability has been revealed. It is shown that the culture is a sensitive model for investigation of physiological interaction of neutrophils with muscle tissue cells.

Animals↗
PubMed1992

[Changes in creatine kinase activity and protein spectrum of the developing muscle culture treated with creatine phosphate].

A study was made of the influence of creatine phosphate (CP) on creatine kinase activity and the total protein status of the primary chicken myoblast culture. It has been established that the two-hour exposure of the developing culture in the presence of 2 and 4 mM CP is accompanied by the lowering of the content of water extractable protein and a considerable increase of creatine kinase activity. Meanwhile the total activity of the enzyme rises in the non-differentiated and falls in the differentiated one. Electrophoretic analysis has shown CP to produce not only quantitative but also deep qualitative changes in the myoblast protein spectrum. It is assumed that the changes revealed are nonspecific.

Animals↗
PubMed1992

[The mechanisms of the neutrophil suppression of creatine kinase and aspartate aminotransferase activities in rat skeletal muscles].

Abdominal neutrophils effect on rat skeletal muscle m. soleus was investigated in vitro. The incubation was carried out in Hanks balanced solution within 24 hrs. It was a release of proteins from m. soleus 1 hr later. Creatine kinase (CK) and aspartate aminotransferase (AAT) activities increase was detected in incubation medium. The neutrophils released their proteins quicker than muscles. A dramatic inhibition of CK and AAT activities took place during coincubation of m. soleus and neutrophils. Zymosan-activated cells had a higher inhibition potency in comparison to nonactivated neutrophils. Analysis of proteinase and myeloperoxidase activities in incubation medium has given evidence that CK and AAT inhibition by non-activated neutrophils mainly depends on cell-secreted proteinases. Zymosan-activated neutrophil inhibition of CK and AAT consists of proteinases and myeloperoxidase effects. AAT appeared to be more resistant than CK to the damage by neutrophils. The used approach failed to demonstrate the direct damage effect of neutrophils on m. soleus, but the described enzyme inhibition mechanism can take place in vivo during leukocyte infiltration of skeletal muscles after intensive muscular activity.

Animals↗
PubMed1988

[Lymphopoietic characteristics in acute hypobaric hypoxia].

10 healthy men were examined, that underwent a 48 hours hypobaric (acute) hypoxia in the climate chamber "Tabaĭ", with conditions corresponding to those at an altitude of 4,000 m. The "ascent" and "descent" were performed correspondingly within 30 minutes. Increased share of dying lymphocytes and frequency of chromosomal aberrations in these were found in addition to an elevated activity of nucleolar organizers and to changed morphological patterns of argentophilic nucleoli. T- and B-lymphocyte contents remained unchanged. The role of the lymphopoietic alterations in autoimmune complications and of hemopoietic disorders in the mountains is discussed.

Acute Disease↗
PubMedDate not supplied

[The kinetics of the urinary excretion of mexidol and its glucuronate conjugate in patients].

Using HPLC with fluorescence detector we studied elimination kinetics of the antioxidant drug mexidol and its glucuronoconjugated metabolite in 16 patients suffering from neurosis and traumatic CNS injury after receiving mexadol (in a dose of 500 mg, in tablets). It is found that mexidol undergoes rapid glucuronoconjugation and is excreted with urine (0.31% and 49.6% of the received dose in the form of mexidol and its glucuronoconjugate, respectively) within 11.5-h period of examination. A substantial variability in the rate and degree of mexidol and glucuronoconjugate elimination with urine is observed. A trend to intensive glucuronoconjugation is observed in patients aged up to 40.

Adult↗
PubMedDate not supplied

[Effects of natural and artificial defoliations on the content and composition of extractive substances in birch leaves].

Qualitative and quantitative compositions of extracts of birch (Betula pendula Roth.) leaves after natural and artificial defoliations were studied. Composition of the fraction of total lipids was determined. Overall, 11 fatty acids were identified. Differences between the fatty acid compositions of total lipids in the trees subjected to defoliation, consisting in the increase in quantities of short-chain saturated fatty acids and trienoic acids, were detected. Nine individual compounds--six flavones, two flavanones, and one flavanonol--were isolated from the flavonoid fraction by column chromatography. It was found that the total content of extractive substances in birch leaves as well as amounts of free sterols, triterpene compounds, and flavones decreased 1 year after an artificial defoliation and 1 month after depredation of 75% of birch stands by gypsy moth. On the contrary, the contents of flavanones and flavanonol increased. The assay method proposed may be used for studying the compositions of plant extracts.

Betula↗
PubMedDate not supplied

[Immunohematologic changes in pituitary adenomas].

The authors conducted studies of colony-producing capacity of precursor cells of bone marrow granulomonopoiesis and cytogenetic study of blood T lymphocytes in 13 patients with adenoma of the hypophysis. It was established that adenomas of the hypophysis are attended by the development of functional deficiency of T lymphocytes and the resultant disturbance of T cell regulation of granulomonopoiesis. The disorder of granulomonopoiesis occurs at the level of the precursor cells in the bone marrow.

Adenoma, Acidophil↗