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Biomedical subjects

T Nogami

Publications and source records attributed to T Nogami.

At least 37 records · Page 2Linked to original sources
PubMed1997

Expression of Bcl-2 in human breast cancer: correlation between hormone receptor status, p53 protein accumulation and DNA strand breaks associated with apoptosis.

The expression of Bcl-2, a suppressor of apoptotic cell death, was investigated in 52 invasive carcinomas of the breast using reverse transcription-polymerase chain reaction and immunohistochemical methods. After consideration of both sets of results, 42 tumors (80.8%) were confirmed to be positive (Bcl-2(+)) and 10 (19.2%) were judged negative (Bcl-2(-)) for Bcl-2 expression. Related factors (p53 protein accumulation, hormone receptor status and apoptotic cell index) were also examined using immunohistochemical and in situ end-labeling methods to elucidate their correlations with Bcl-2 expression. Bcl-2 expression correlated significantly with the hormone receptor status, whereas it showed significant inverse correlations with p53 accumulation and the apoptotic index. It was concluded that estrogen and mutant p53 are related to the regulation of Bcl-2 expression and that the ability to prevent tumor cell death due to Bcl-2 can be developed by breast cancers.

Apoptosis↗
PubMed1997

[A case of complete remission of gastric endocrine cell carcinoma with multiple bone metastasis by combination chemotherapy and high-dose chemotherapy with autologous peripheral blood stem cell transplantation].

A 31-year-old man was admitted to our hospital complaining of epigastric discomfort and severe lumbago. An upper gastrointestinal endoscopy revealed several submucosallike tumors. Histologic examination of biopsy specimens confirmed the presence of endocrine cell carcinoma. Gallium scintigraphy and CT revealed multiple bone metastasis. He was treated with 6 cycles of combination chemotherapy consisting of CDDP, etoposide, CPA, EPI and VCR. Both gastric tumors and bone metastasis completely disappeared. After 7 cycles of the chemotherapy, he was treated with HDCT with PBSCT. There was no severe complication. This result suggested that the combination of conventional chemotherapy and HDCT with PBSCT was useful in cancer patients with poor prognoses, such as advanced gastric endocrine cell carcinoma.

Adult↗
PubMed1995

[Scimitar syndrome with diagnostic MRI findings].

A fifty-year-old woman was admitted to our hospital because of an abnormal shadow on a chest X-ray film. She was asymptomatic. Chest X-ray film showed a scimitar-like vessel in the right lung, dextroposition of the heart, and hypoplasia of the right pulmonary artery. MRI revealed that the scimitar vein was anomalous pulmonary venous return from the right lung. Normal venous return from the right lower lobe to the left atrium was confirmed by pulmonary artery angiography. MRI findings have been useful in the diagnosis of scimitar syndrome.

Female↗
PubMed1992

Premenstrual asthma with seasonal variation.

A 19-year-old woman had premenstrual asthma (PMA) usually from April through October each year with normal and regular menstrual cycles. When the monthly variation in the patient's PMA between 1984 and 1990 was compared with the monthly admissions of children for acute asthma in a hospital in this region, there was a great similarity in pattern between the two. Although she had high sensitivity to house-dust mites, the monthly pattern of her PMA did not coincide with monthly variations in the number of mites in house-dust in her home.

Administration, Inhalation↗
PubMed1990

[A fundamental study of immunoscintigraphy with 131I-labeled anti-CA 19-9 and anti-CEA monoclonal antibodies--imaging of tumor-bearing mice by IMACIS-1 and cell ELISA with human tumor cells].

A study was made on 2 types of 131I-labeled anti-CA 19-9 and anti-CEA mouse monoclonal antibodies (IMACIS-1) against human cancer related antigen as to their usefulness in radioimmunoimaging. Tumor-bearing nude mice were used for comparison. The transplanted tumors (SW948, COLO 201) were clearly visualized 48-72 hours after administration of IMACIS-1. Tumor/blood ratio 72 hours after administration: 8.69 in COLO 201 and 5.70 in SW948, showing ca. 10-15 times as high as those in PC-3 and HEp-2. IMACIS-1 therefore is considered useful in radioimmunoimaging of cancer. Analysis was made by in vitro cell ELISA. As a result, both of the cells specifically reacted with anti-CA 19-9 but not anti-CEA.

Animals↗
PubMed1988

Inhibition of human immunodeficiency virus-associated reverse transcriptase by aminoacridines.

A semi-synthetic bleomycin analogue and its DNA binding moiety, an alkylamino-2-methoxy-6-chloroacridine, have been found to inhibit human immunodeficiency virus-associated reverse transcriptase. The authors have synthesized various new alkylaminoacridine derivatives. Alkylaminoacridines with basic functional groups showed the enzyme inhibitory activity with less cytotoxicity.

Aminoacridines↗
PubMed1987

Viral replication in HeLa/fibroblast hybrid cells infected with human cytomegalovirus.

Three human hybrid cell lines were generated by the fusion of D 98OR, a HeLa cell variant, and TIG human diploid fibroblasts. Chromosome numbers of the hybrid cells fell between that of D 98OR cells and the combined chromosome number of the two cell lines, with three marker chromosomes identical to those of D 98OR cells. One hybrid, B-3, produced large amounts of human fibronectin as analyzed by immunohistochemistry. Only human cytomegalovirus (HCMV) infected B-3 cells showed positive fluorescence as detected by human antiserum to HCMV. Further cloning of B-3 by limiting dilution resulted in two cloned hybrids with markedly enhanced virus production as compared with B-3 cells. Treatment of these two clones with phorbol ester further enhanced virus production. These cloned hybrids may provide a tool to analyze host cell factors controlling the transcription and replication of HCMV.

Antigens, Viral↗
PubMed1987

Induction of S-100b (beta beta) protein in human teratocarcinoma cells.

Human teratocarcinoma NT2/D1 cells undergo differentiation into a variety of cell types, including neurons, treated with retinoic acid. In the present study, the concentrations of alpha S-100 and beta S-100 proteins (alpha and beta subunits of S-100 proteins), and three subunits (alpha, beta and gamma) of enolase in NT2/D1 cells were measured using the sensitive enzyme immunoassay method. The concentration of beta S-100 was markedly increased in the cells after treatment with retinoic acid, whereas the concentration of alpha S-100 was undetectably low, indicating that the S-100b (beta beta) protein was induced by retinoic acid. On the other hand, the concentrations of the three forms of enolase isozymes did not change in the same culture. The induction of S-100b protein was not observed in the NT2/D1 cells after treatment with forskolin, dibutyryl cyclic AMP or cholera toxin. The indirect double-labeled immunofluorescence, using antibodies specific to beta S-100 and monoclonal antibodies specific to neurofilaments, revealed that both the S-100b protein and the neurofilaments were induced in the same subpopulation of cells which underwent neuronal differentiation.

Antibodies, Monoclonal↗
PubMed1985

Extracorporeal lung assist with a single catheter in puppies.

We devised a new veno-venous extracorporeal bypass circuit to be used with an artificial membrane lung for respiratory support in the newborn. A roller pump was activated intermittently when the reservoir filled. Capacitance detectors applied to the reservoir regulated blood flow through a thin-walled venous catheter. Extracorporeal lung assist with this circuit was tested on puppies ventilated with air at a rate of 2 to 4 breath/ min. At a mean blood flow rate of 37.2 +/- 12.1 ml/kg min, PaO2 and PaCO2 were maintained in the normal range. Veno-venous extracorporeal lung assist with a single venous catheter may well become an excellent means of neonatal respiratory support in the future.

Animals↗
PubMed1985

Construction of a series of ompF-ompC chimeric genes by in vivo homologous recombination in Escherichia coli and characterization of the translational products.

OmpF and OmpC are major outer membrane proteins. Although they are homologous proteins, they function differently in several respects. As an approach to elucidate the submolecular structures that determine the difference, a method was developed to construct a series of ompF-ompC chimeric genes by in vivo homologous recombination between these two genes, which are adjacent on a plasmid. The genomic structures of these chimeric genes were determined by restriction endonuclease analysis and nucleotide sequence determination. In almost all cases, recombination took place between the corresponding homologous regions of the ompF and ompC genes. Many of the chimeric genes produced proteins that migrated to various positions between the OmpF and OmpC proteins on polyacrylamide gel. On the basis of the results, a domain contributing to the mobility difference the OmpF and OmpC proteins was identified. Some chimeric genes did not accumulate outer membrane proteins, despite the fact that the fusion of the ompF and ompC genes was in frame. Bacterial cells possessing the chimeric proteins were also tested as to their sensitivity to phages which require either OmpF or OmpC as a receptor component. The chimeric proteins were either of the OmpF or OmpC type with respect to receptor activity. Based on the observations, the roles of submolecular domains in the structure, function, and biogenesis of the OmpF and OmpC proteins are discussed.

Bacterial Outer Membrane Proteins↗