Evidence for continuous availability of the phosphoribosylglycinamide synthetase structural gene for transcription during the cell cycle of the eucaryote Chlorella.
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Amino Sugars↗
Biomedical subjects
T O Sitz
Publications and source records attributed to T O Sitz.
Purification of Synechococcus lividus by equilibrium centrifugation and its synchronization by differential centrifugation.
A culture of the thermophilic, unicellular, blue-green alga Synechococcus lividus was freed from two thermophilic bacteria by use of equilibrium (isopycnic) centrifugation in Ficoll density gradients. After removal of the bacteria, the alga would grow only on agar plates in a high carbon dioxide atmosphere. Intermittent illumination, equilibrium centrifugation, and differential centrifugation were tested as techniques for obtaining synchronized cultures of the alga. Daughter cells selected by differential centrifugation in Ficoll density gradients yielded the best synchrony, and a cross-wall index of 85% was observed during the period of cell division.
Agar↗
Similarity of ribosomal and ribosomal precursor RNA's from rat liver and the Novikoff ascites hepatoma.
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Animals↗
Synchronous culture production by density selection.
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Animals↗
Equilibrium density-gradient procedure for selection of synchronous cells from asynchronous cultures.
A reproducible isopycnic technique, in which linear density gradients of Ficoll are used, was developed for selecting synchronous daughter cells from asynchronous cultures of Chlorella for biochemical studies of the cell cycle.
Cell Division↗
Selection of synchronous Chlorella cells by centrifugation to equilibrium in aqueous Ficoll.
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Cell Division↗