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T Oite

Publications and source records attributed to T Oite.

86 records · Page 5Linked to original sources
PubMed1980

Culture of isolated glomeruli from normal and nephritic rabbits. I. Characterization of outgrowing cells.

The existence of four types of cells in the glomerular outgrowths from both normal and nephritic rabbits was confirmed, and their characteristics by phase contrast, scanning electron, and transmission electron microscopy are described. Type I (arborized), Type II (fusiform), Type III (round), and Type IV (paving stone-like) cells presumably originate from visceral epithelial cells, mesangial cells, monocytes, and parietal epithelial cells of Bowman's capsule, respectively, though conclusive evidence is still lacking. The Type III cells, unlike the other types of cells, have a remarkable phagocytic capacity as well as Fc and C3 receptors.

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PubMed1980

Culture of isolated glomeruli from normal and nephritic rabbits. II. Kinetics of outgrowing cells.

In the autologous phase of nephrotoxic nephritis in the rabbit, many round (Type III) cells appeared in the glomerular outgrowth. They had receptors for Fc and C3 and a pronounced phagocytic capacity for carbon. The number of Type III cells in the glomerular outgrowth from rabbits 8 days after injection of nephrotoxic serum (NTS-8) was higher than that of NTS-10 or -15. In contrast, they were rarely observed in the outgrowth of glomeruli from normal and NTS-3 rabbits. The Type II (fusiform) cell, a type of intrinsic glomerular cell, was also increased earlier and more markedly in NTS-8, -10, and -15 than in normal and NTS-3 rabbits.

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PubMed1980

Anionic binding sites in the glomerular basement membrane: possible role in the pathogenesis of immune complex glomerulonephritis.

This paper details the ways in which the anionic sites of the GBM could be involved in immune complex deposition. Experiments were performed with chemically cationised proteins to test the hypotheses proposed. The most detailed investigations were concerned with the role of basic (cationic) antigen as a planted antigen, leading to in situ immune complex formation. A model was developed where it was possible to induce marked proteinuria following a single injection of small quantities of cationised human IgG or ferritin.

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PubMed1980

Role of divalent cations in binding polymorphonuclear leucocytes to glomeruli with immune complexes.

The binding of polymorphonuclear leucocytes (PMN) to the glomeruli of rats in which glomerulonephritis had been induced by bovine serum albumin, suggested a biological activity of the immune complexes localized in the glomeruli. To evaluate divalent cations or serum factors which may be involved, a two-step method for PMN binding was introduced. PMN were suspended in serum-free media and incubated with cryostat kidney sections pretreated with divalent cation-chelated or fresh sera. An appropriate concentration of Mg++ was found to be essential for maximal PMN binding; however, Ca++ may represent a partial substitute for Mg++. Immune complexes localized in the glomeruli fix and activate the complement system via both the classical and the alternative pathways.

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PubMed1979

Attachment of polymorphonuclear leukocytes to glomeruli with immune deposits.

A method for quantitative evaluation of a biological activity of immune complexes deposited in glomeruli is described. The activity reflects the activation of complement and is represented by the number of PMN attached to a glomerulus. It is possible to compare data from different individuals or different phases of glomerulonephritis. The complement component concerned is considered to be C3, activated through the classical or alternate pathway.

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PubMed1979

Accelerated immune complex nephritis due to mesangial overloading in spontaneous hypertensive (SHR) rats.

Immune complex nephritis induced by bovine serum albumin (BSA) and horseradish peroxidase (HRP) was superimposed upon spontaneous hypertensive rats (SHR rats) which were pretreated with polyvinyl alcohol (PVA). PVA accumulated predominantly in the mesangium, and the superimposed nephritis developed more accelerated glomerular damage with marked capillary deposition of immune complexes than control animals which were not pretreated with PVA.

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PubMed1979

Masugi nephritis in T cell-depleted state. Influence of adult thymectomy and anti-thymocyte serum.

The effects of surgical removal of the thymus or the administration of antiserum to thymus-derived lymphocytes on the development of Masugi nephritis were investigated in Wistar rats. While thymectomy at weaning (ATx) had no significant effect on the humoral antibody response to injected rabbit IgG, repeated injections of rabbit anti-rat thymocyte serum (ATS) suppressed it remarkably. Both treatments did not show morphological evidences that glomerular inflammation and injury were suppressed in the autologous phase of Masugi nephritis. Glomerular lesions in rats receiving the nephrotoxic serum (NTS) one month after ATx (ATx-1+NTS) appeared to be severer in hypercellularity, mitotic counts, the amount of deposited fibrin-related substances and crescent formation than those in other nephritic groups. Morphological study of ATS+NTS rats revealed that the glomerular changes were nearly equal to those of NTS-injected control rats, in spite of markedly suppressed humoral response to injected rabbit IgG and the absence of host IgG along the glomerular capillary walls.

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PubMed1979

Immunoassay with fluorescein-labelled antigens--device of a method and its clinical application.

A simple, sensitive, highly reproducible method for assay of antibodies using fluorescein-labelled antigens according to the method by Farr is described. We measured titers of anti-BSA antibodies and antigen binding capacity of sera in BSA immunized rabbits by fluorometric assay with fluorescein isothiocyanate (FITC) labelled BSA, and obtained highly reproducible and quantitative results. The resultd times more sensitive than the results from single radial immunodiffusion. FITC-labelled double or single stranded calf thymus DNA was incubated with sera of patients with systemic lupus erythematosus and New Zealand Black mice. Both anti-double and single stranded DNA antibodies were detected in sera of systemic lupus erythematosus, but only anti-single stranded DNA antibodies in sera of New Zealand Black mice.

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PubMed1978

Bovine serum albumin (BSA) nephritis in rats. I. Experimental model.

Proliferative glomerulonephritis with proteinuria was induced in Wistar rats by bovine serum albumin (BSA). Rats were first immunized with 1 mg or 2.5 mg of BSA and complete Freund's adjuvant (CFA) and eight weeks later 1 mg of BSA were given intravenously six times a week for four weeks. Immunofluorescence revealed granular deposits of IgG, C3, and BSA in the mesangial area with or without deposition of the same components along the capillary wall. Evaluation of the circulating antibody disclosed an apparent correlation between the level of antibody and histological findings. Rats with an intermediate amount of antibody production developed mesangial widening with mesangial immune deposits and no proteinuria. Rats with a low response developed proliferative glomerulonephritis with immune deposits along the capillary wall as well as in the mesangial area and proteinuria.

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PubMed1976

Participation of blood born cells in rat Masugi nephritis.

Participation of blood born cells in rat Masugi nephritis was investigated with ultrastructural demonstration of peroxidase, in addition to conventional light and electron microscopies. Polymorphonuclear leukocytes appear immediately and transiently after injection of nephrotoxic serum. Hypercellularity in the early stage of the disease is consisted mainly of monocyte-macrophage. Proteinuria and infiltration of few monocytes are persistent through the course up to 124 days and focal sclerosis with hyaline material appears in the later stage.

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PubMedDate not supplied

Sensitive analysis of apoptosis using confocal laser scan microscopy.

Application of the TUNEL method and immunostaining cell-specific markers to a whole isolated glomerulus in combination with confocal laser scan microscopy can be used to analyze cell turnover including apoptosis within glomeruli. Furthermore, the technologies can be used to deepen our understanding of glomerular cell biology and pathophysiology at the cellular and molecular levels.

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PubMedDate not supplied

A specific Thy-1 molecular epitope expressed on rat mesangial cells.

An experimental model of proliferative glomerulonephritis induced by antibodies against the Thy-1 antigen has been established and used to study the pathological sequence from mesangiolysis to mesangial proliferation. However, the functional role of the Thy-1 molecule distributed on rat glomerular mesangial cells remains unknown, so the present study was undertaken to determine the precise subcellular localization of Thy-1 molecules in vitro using two anti-Thy-1 monoclonal antibodies, 1-22-3 and OX-7. Immunofluorescence and immunoelectron microscopic studies in combination with laser scanning analysis showed that the localization of 1-22-3 and OX-7 bound to cultured rat mesangial cells differed, particularly when cocultured with vascular endothelial cells. An epitope recognized by 1-22-3 was concentrated specifically on the mesangial cell surfaces facing the neighboring endothelial cells. In contrast, OX-7 bound to mesangial cell surfaces and extracellular parts in a diffuse pattern independent of contact with endothelial cells. This finding is consistent with our previous ultrastructural study in which the reactivity of 1-22-3 with normal kidney tissue was limited to mesangial cell surfaces facing endothelial cells. These results led us to conclude that the specific Thy-1 molecular epitope recognized by 1-22-3 is associated with points anchoring mesangial and endothelial cells, where this anti-Thy-1 antibody binds after injection in vivo, resulting in mesangial cell detachment from the vascular capillary wall, mesangiolysis and mesangial cell dysfunction. We believe that the critical epitope detected by 1-22-3 in this study plays an important role in mesangial cell function and injury.

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