Tubular markers in renal transplant patients under cyclosporine.
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Biomedical subjects
Publications and source records attributed to T Quiroga.
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Since the 1930's peripheral blood cell counts are among the most common clinical tests contributing to diagnosis of a wide variety of diseases. The original Schilling formula, based on findings in a European population at the beginning of this century are still used as reference in our country. We therefore report the values found in a population of both sexes without manifest disease and qualifying to be blood donors. Means, 5 and 95 percentiles for 212 males and 43 females obtained with the use of a Technicon H6000 are reported.
Pseudo-hyponatremia is a rare condition characterized by a decrease in plasma sodium concentration associated with a normal or increased effective plasma osmolarity. We present the case of a 62 year old woman with multiple myeloma (IgG) who had a plasma sodium of 84.5 mEq/l (as measured with flame photometry) and an anion gap of -8.8 mEq/l. However, when determined with an ion-selective electrode, plasma sodium was 135 mEq/l. The patient was treated with chemotherapy and did not receive sodium. One week later, plasma sodium--again measured by flame photometry--was 134 mEq/l and the anion gap 4.0 mEq/l. The pathophysiological and clinical aspects of pseudo-hyponatremia and the anion gap are discussed.
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Serum samples from 85 patients with proven typhoid fever, 11 patients with p-typhoidal fever, 101 patients with febrile non-typhoidal, and 130 healthy subjects were tested for immunoglobulin G (IgG), IgA, and IgM antilipopolysaccharide (LPS) of Salmonella typhi antibodies by enzyme-linked immunosorbent assay (ELISA) and Widal test. The levels of all three classes of immunoglobulin anti-LPS of S. typhi were higher in typhoid patients than in healthy or febrile nontyphoidal groups; we selected various combinations between the three classes of immunoglobulin to obtain the best combination of sensitivity and specificity. The sum of the absorbance values obtained from the ELISA assay for IgG+IgA+IgM (sigma lgs) was the best choice for diagnostic utility for typhoid fever. We selected a positive test at a decision level of sigma lgs > or = 1.2 with a sensitivity of 94% and a specificity of 92% with a frequency of false negative of 5.9%. The frequency of false positives for healthy controls was 7.7% and, for the febrile nontyphoidal group, it was 7.9%. We also compared receiver (or relative) operating characteristic (ROC) curves for the diagnostic usefulness of the ELISA with that of the Widal test, whose merits and limitations, especially in endemic regions, are discussed. The ELISA assay was much more sensitive and specific than any combination of the Widal test, and hence it could be a useful tool for the serologic diagnosis of typhoidal fever with a single blood sample.