Infectious mononucleosis and syphilis testing: a similar history and future.
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Biomedical subjects
Publications and source records attributed to T R Fairbanks.
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The determination of lymphocyte subpopulations is becoming increasingly useful in the clinical laboratory. Defining cells beyond the T- and B-lymphocyte level is now possible and with improved technology such as double labeling, histochemical stains, and cytofluorographic analysis, lymphocyte subpopulation identification will become more routine. This overview presents the current status of the art of defining lymphocyte subpopulations and its usefulness in clinical medicine.
A simple, inexpensive, and easily visible procedure for labeling phagocytic cells in B-cell preparations is discussed. The procedure involves adding rhodamine-latex particles to lymphocyte preparations, then simultaneously looking for the presence of ingested latex particles in contaminating phagocytic cells and for fluorescein labeled surface immunoglobulins. This differentiation of cells allows one to correct for the true percentage of B cells.
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