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T R Famula

Publications and source records attributed to T R Famula.

46 records · Page 3Linked to original sources
PubMed1986

Identifying single genes of large effect in quantitative traits using best linear unbiased prediction.

The recent discovery of a major gene for rapid postweaning growth has reinforced the hypothesis that other quantitative continuous traits may be influenced by single genes of large effect. However, most methods for the detection of such genes rely on the discovery of multimodality in the population frequency distribution. The complicating effects of environment and artificial selection make the identification of such genes with field-collected data a formidable problem. An index is proposed that may serve as an indicator that a major gene is segregating within a population. The index is based on the assumption that under polygenic inheritance, an offspring's deviation from the midparent average is smaller than the deviation from either parent. Whereas, for the Mendelian segregation of a major gene, the opposite would be expected. A proposed class of indices is then based on the ratio [O - .5(S + D)]k/([O - S]k/2 [O - D]k/2) where O, S and D are the additive genetic values of an offspring and its sire and dam estimated via best linear unbiased prediction. Values of the index greater than 1. would be indicative of major gene inheritance. Simulation of small populations indicates that the index is quite sensitive to the existence of segregating major genes even in the absence of multimodality of the phenotypic distribution. However, the index remains dependent on the accuracy of genetic value estimation.

Animals↗
PubMed1985

Ovulation rate in sheep selected for weaning weight or litter size.

Ovulation rate was measured by laparoscopy at two consecutive cycles on 366 ewes 2 yr old and over and 85 yearling ewes of five lines of Targhees from the base base population; 53 yearling linecross ewes were also included. The lines were two unselected controls (HCl and DC), two selected for 21 yr for increased 120-d weight (HW and DH) and one selected for 19 yr for multiple births (T). Ewes were synchronized in late July or early August at the start of the normal breeding season with intravaginal pessaries impregnated with 60 mg methylacetoxyprogesterone and examined at first and second estrus. Ovulation had occurred in both cycles in 327 (89%) and 177 (85%) of the mature and yearling ewes, respectively. Overall mean numbers of corpora lutea at first and second estrus were 1.42 and 1.63, respectively for ewes 2 yr and over and 1.20 and 1.44 for yearlings, indicating an effect of synchronizing treatment, season, flushing, or a combination of these. Among mature ewes, ovulation rate was higher (P less than .05) in DH (+.20), HW (+.19) and T (+.16) than in controls at first estrus, and in HW (+.29) and T (+.21) but not DH (-.04) at second estrus. Among yearlings, differences were significant only at second estrus (HW, +.40; T, +.35) and again not for DH (+.08). The failure of line DH to increase in ovulation rate from first to second estrus as did other lines was transmitted to linecross progeny. Body weight within line affected ovulation rate significantly, with a greater effect at second estrus, in both age groups. Adjustment for body weight removed the difference between HW and controls but not between T and controls. Repeatability of corpora lutea count was .27 and .25 for mature and yearling ewes, respectively.

Animals↗
PubMed1985

Association of sire dystocia transmitting ability with progeny linear type traits in Holsteins.

Data on 13 traits of 11,260 progeny of 775 sires in the Carnation Genetics linear type appraisal program were analyzed to determine the association between sire dystocia transmitting ability and progeny linear type traits. Mean linear type scores ranged from 25.0 to 29.8 on a scale of 1 to 50. Parity, stage of lactation, and interactions of evaluator by parity and evaluator by stage were sources of variation for most traits. First quartile (easy) and fourth quartile (most difficult) sires grouped by dystocia transmitting ability differed in daughter linear type scores for stature, dairy character, rump angle, center ligament, udder depth, teat length, teat placement, and rump width. Differential use of easy calving bulls on heifers might result in progeny with lower type scores for stature, dairy character, rump angle, legs, and rump width. Average type scores differed by less than 1 unit for all traits, indicating little correlated response in linear type traits through use of bulls whose progeny are born with least difficulty.

Analysis of Variance↗
PubMed1985

Serial composition during growth in mice with a major gene for rapid postweaning growth.

The chemical composition of growth in mice having a major gene for rapid post-weaning growth (hg/hg) was compared to that of their normal counterparts (Hg/-) at repeated intervals from 21 to 57 days of age. Male mice of both genotypes were sacrificed at 3 day intervals from 21 to 42 days of age and at 5 day intervals thereafter to day 57 of age for determination of body composition. By day 57 the hg/hg mice weighed 44.2 g while the Hg/- mice weighed 31.9 g. Analysis of changes in body water, protein, fat and ash across ages demonstrated that the hg/hg mice had significantly greater percents of body water and fat while having less protein and ash as compared to the Hg/- mice. When the effect of body weight was removed by covariance analysis, the difference in body lipid between genotypes was no longer significant. The hg/hg mice continued to maintain a slightly but significantly greater amount of water in the body while protein and ash levels remained lower than in Hg/- mice. Age also significantly influenced the amount of water, protein and ash in both mouse genotypes. It was concluded that the very slight, but significant differences in amount of water, protein and ash in the hg/hg mice were the result of a slower rate of maturing. The gene (hg) seems to have a general influence on all chemical components of growth and apparently does not alter any specific chemical component of growth.

Aging↗
PubMed1984

Composition of growth in mice with a major gene for rapid postweaning gain.

Previous research from this laboratory identified the existence of a major gene for rapid growth in mice. This trait is expressed as rapid postweaning growth and is inherited as a single recessive gene, designated hg. The hg/hg males and females had greater (P less than .05) body weights at 49-d of age as compared with their Hg/-counterparts; 45.6 +/- .64 and 34.0 +/- .64 vs 33.2 +/- .23 and 27.3 +/- .15 g, respectively. Twenty-four male littermate pairs (hg/hg and Hg/-) were euthanized at 49 d of age for determination of carcass composition. Percentage body water was not affected by genotype (hg/hg, 66.7 +/- .5; Hg/-, 67.8 +/- .3). However, there was a decrease (P less than .05) in percentage body protein (20.2 +/- .1 vs 20.8 +/- .1) and body ash (3.4 +/- .01 vs 3.7 +/- .01) in hg/hg males as compared with Hg/- males, while percentage lipid was significantly increased in hg/hg males (8.7 +/- .5 vs 6.7 +/- .3). When carcass weights were adjusted for differences in dry weight, only body protein was significantly affected by genotype, being greater for hg/hg males (8.54 +/- .19 vs 7.70 +/- .19 g). All other components of body composition were not affected by genotype. The regression coefficient for the covariate of dry weight was significantly different from zero for all carcass components. However, there was no significant difference in the regression coefficient between Hg/- and hg/hg males for each component.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMedDate not supplied

Analysis of genetic variation in 28 dog breed populations with 100 microsatellite markers.

Dog breeds were created by man choosing for select phenotypic traits such as size, shape, coat color, conformation, and behavior. Rigorous phenotypic selection likely resulted in a loss of genetic information. The present study extends previous dog population observations by assessing the genotypic variation within and across 28 breeds representing the seven recognized breed groups of the American Kennel Club (AKC). One hundred autosomal microsatellite markers distributed across the canine genome were used to examine variation within breeds. Resulting breed-specific allele frequencies were then used in an attempt to elucidate phylogeny and genetic distances between breeds. While the set of autosomal microsatellites was useful in describing genetic variation within breeds, establishing the genetic relatedness between breeds was less conclusive. A more accurate determination of breed phylogeny will likely require the use of single-nucleotide polymorphisms (SNPs).

Animals↗
PubMedDate not supplied

The genetics of epilepsy in the Belgian tervuren and sheepdog.

Idiopathic epilepsy is characterized by recurrent seizure activity without an identifiable underlying anatomic defect. Dogs experiencing repeated bouts of severe seizures are given therapeutic medication to control their frequency and severity. Idiopathic epilepsy has been reported in many dog breeds and was identified as the predominant health issue facing dog breeds in a recent survey by the American Kennel Club. A growing body of evidence supports a hereditary basis for idiopathic epilepsy, with a variety of genetic inheritance models proposed. In the Belgian tervuren and sheepdog, epilepsy is highly heritable with a polygenic mode of inheritance, though apparently influenced by a single autosomal recessive locus of large effect. In an effort to establish molecular linkage between the epileptic phenotype and the locus of large effect, we have screened genomic DNA from families of affected tervuren and sheepdogs with 100 widely dispersed, polymorphic canine microsatellite markers (0.595 average PIC value). Although not significant (LOD scores <3.0), three genomic regions have shown nominal linkage between markers and the epileptic phenotype. Additional related dogs are being screened with these and additional markers to increase the power to detect the presence of a linked locus.

Animals↗
PubMedDate not supplied

Inheritance of urinary calculi in the Dalmatian.

Dalmatians are unique among dogs in that they excrete uric acid in their urine as the end product of purine metabolism rather than allantoin as do other breeds of dogs. Urinary calculi form from urate (salts of uric acid) and can cause urethral obstruction in male Dalmatians. Although all Dalmatians have the primary defect, only a subset develops clinical disease. We postulated that calculi formation might have a genetic component that segregates within the breed, causing some animals to form calculi and others to never form calculi despite excreting uric acid in their urine. We used a survey to ascertain the urinary calculi status based on clinical signs of adult Dalmatians aged 6 years or older, and we used pedigrees from these same animals to estimate the heritability of the clinical manifestation of urate calculi within the breed to be .87 (.75-.96). The prevalence of the disease was 34% (24.99-43.70%) among male Dalmatians in our survey. The high heritability of the disease makes it possible for breeders to effectively select against the disease.

Animals↗
PubMedDate not supplied

Effects of the high growth (hg) mutation on reproduction in high growth (HG) female mice.

Reproductive performance was characterized in females of a line of mice homozygous for a recessive mutation with a major effect on growth, the high growth (hg) mutation. Specifically, the age at onset of puberty, length of the estrous cycle, ovulation rate, and gestation length in high growth (HG) mice were determined and compared with control mice. HG females reached puberty at an age similar to control animals, exhibited normal ovulation rates and estrous cycle length, but had a shorter length of estrus. Number of fetuses at Day 17 of gestation was greater and gestation length was longer in HG females. The HG females exhibited a variety of reproductive disorders, including increased incidence of repeated detection of mating plugs without intervening pregnancy ("replug"), increased incidence of repeated detection of mating plugs within an interval of 1-8 days without intervening pregnancy ("recycle"), increased interval between pairing with a male and detection of the first mating plug, increased interval between mating and conception, increased postnatal mortality, decreased litter size, and increased incidence of vaginal septum.

Animals↗